Date published: 2025-9-7
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FGF-21 Antibody (Y-16) - Western Blotting - Image 33597
FGF-21 抗体 (Y-16): sc-81946。对 HeLa 全细胞裂解液中 FGF-21 的表达进行 Western 印迹分析。
FGF-21 Antibody (Y-16) - Western Blotting - Image 33597
FGF-21 抗体 (Y-16): sc-81946。对 HeLa 全细胞裂解液中 FGF-21 的表达进行 Western 印迹分析。
FGF-21 Antibody (Y-16) - Western Blotting - Image 30058
FGF-21 抗体 (Y-16): sc-81946. 未转染 293T: sc-117752 (A), 转染人 FGF-21 的 293T: sc-113648 (B) 和 HeLa (C) 全细胞裂解液中 FGF-21 表达的 Western 印迹分析.
FGF-21 抗体 (Y-16): sc-81946。对 HeLa 全细胞裂解液中 FGF-21 的表达进行 Western 印迹分析。

FGF-21 抗体 (Y-16): sc-81946

3.9(8)
寫評論提問

说明书
  • FGF-21 抗体 Y-16 是小鼠单克隆 IgG1 κ，FGF-21抗体, 在9篇文献中引用，规格为100 µg/ml
  • 免疫human物种的重组FGF-21
  • 推荐用于 human 来源的FGF-21 WB, IP 和 ELISA检测
  • 关于如何获取FGF-21 (Y-16): sc-81946的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是FGF-21 Antibody (Y-16) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与FGF-21 Antibody (Y-16) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外FGF抗体，包括FGF-1, FGF-10, FGF-12, FGF-15, FGF-16, FGF-17, FGF-18, FGF-19, FGF-2, FGF-20, FGF-21, FGF-3, FGF-4, FGF-5, FGF-6和FGF-7

FGF-21抗体（Y-16）是一种IgG1κ小鼠单克隆FGF-21抗体（也称为FGF21抗体），可通过WB、IP和ELISA检测人源FGF-21蛋白。FGF-21抗体（Y-16）可作为非偶联抗FGF-21抗体提供。成纤维细胞生长因子-1（FGF-1），也称为酸性FGF，和成纤维细胞生长因子-2（FGF-2），也称为碱性FGF，是刺激间质、上皮和神经外胚层细胞增殖的生长因子家族成员。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

FGF-21 抗体 (Y-16) 参考文献:

  1. 小鼠乳腺癌细胞依赖雄激素生长所必需的雄激素诱导生长因子的克隆和特征描述。  |  Tanaka, A., et al. 1992. Proc Natl Acad Sci U S A. 89: 8928-32. PMID: 1409588
  2. FGF-21 作为一种新型代谢调节因子  |  Kharitonenkov, A., et al. 2005. J Clin Invest. 115: 1627-35. PMID: 15902306
  3. 克隆和表达与酸性和碱性成纤维细胞生长因子交叉反应的两种不同的高亲和力受体。  |  Dionne, CA., et al. 1990. EMBO J. 9: 2685-92. PMID: 1697263
  4. 成纤维细胞生长因子 21  |  Bass, J. 2013. Nat Med. 19: 1090-2. PMID: 24013744
  5. 碱性成纤维细胞生长因子细胞生物学的最新发展。  |  Rifkin, DB. and Moscatelli, D. 1989. J Cell Biol. 109: 1-6. PMID: 2545723
  6. 成纤维细胞生长因子家族新成员--与 HST 相关的 FGF.6 基因的特征。  |  Marics, I., et al. 1989. Oncogene. 4: 335-40. PMID: 2649847
  7. 通过转染卡波西肉瘤 DNA 分离出的癌基因编码一种生长因子，属于 FGF 家族。  |  Delli Bovi, P., et al. 1987. Cell. 50: 729-37. PMID: 2957062
  8. 小鼠 int-2 的序列, 拓扑结构和蛋白质编码潜力：一种由小鼠乳腺肿瘤病毒激活的假定癌基因。  |  Moore, R., et al. 1986. EMBO J. 5: 919-24. PMID: 3013624
  9. 成纤维细胞生长因子 21 可控制有丝分裂和肌肉质量。  |  Oost, LJ., et al. 2019. J Cachexia Sarcopenia Muscle. 10: 630-642. PMID: 30895728
  10. 人类 FGF-5 癌基因编码一种与成纤维细胞生长因子有关的新型蛋白质。  |  Zhan, X., et al. 1988. Mol Cell Biol. 8: 3487-95. PMID: 3211147
  11. 编码成纤维细胞生长因子家族第九个成员的新型细胞因子 cDNA 的分子克隆，该细胞因子具有独特的分泌特性。  |  Miyamoto, M., et al. 1993. Mol Cell Biol. 13: 4251-9. PMID: 8321227
  12. 小鼠成纤维细胞生长因子 10：cDNA 克隆, 蛋白质鉴定和 mRNA 表达调控。  |  Beer, HD., et al. 1997. Oncogene. 15: 2211-8. PMID: 9393979

订购信息

产品名称产品编号规格价格数量收藏夹

FGF-21 抗体 (Y-16)

sc-81946
100 µg/ml
RMB2504.00

FGF-21 (Y-16): m-IgG Fc BP-HRP 套装

sc-537267
100 µg Ab; 10 µg BP
RMB2662.00

FGF-21 (Y-16): m-IgGκ BP-HRP 套装

sc-534496
100 µg Ab; 40 µg BP
RMB2662.00

FGF-21 (Y-16): m-IgG1 BP-HRP 套装

sc-545118
100 µg Ab; 20 µg BP
RMB2662.00

For Western Blot, is it recommended to use denatured or non-denatured conditions with FGF-21 (Y-16): sc-81946 antibody?

Asked by: jerojero
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by from very good for westernIt gives a strong western blot band for the mouse protein from tissue extract, at 1:1,000 dilution in 3% BSA, PBST.
Date published: 2017-11-03
Rated 1 out of 5 by from Not working in WBThis FGF-21 antibody does not work in WB with human liver.
Date published: 2017-11-03
Rated 4 out of 5 by from Good FGF21 antibody for WB!i used the FGF21 antibody for WB at 1:200 dilution in TBST overnight at 4 degrees on mouse brown adipose tissue samples and the signal was very good and strong !
Date published: 2017-10-23
Rated 5 out of 5 by from Strong FGF21 WB signalsStrong FGF21 WB signals in human cell line were shown with 1:500 dilution in TBST only,after 5%milk blockage 1.5 hours.
Date published: 2017-08-10
Rated 4 out of 5 by from It's ok for WBI used a sample antibody for WB,but the western blot band was too light .1:500 has been tried,Maybe I should increase the conceration and try it again.
Date published: 2017-07-11
Rated 4 out of 5 by from Good!selectly binding to the mouse FGF21,acting a good effect on Western blotting.
Date published: 2016-11-19
Rated 4 out of 5 by from Good specificity in WB in HeLa whole cell lysateGood specificity in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-03-29
Rated 4 out of 5 by from Single strong band in WB in human transfectedSingle strong band in WB in human transfected 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2013-03-31
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FGF-21 抗体 (Y-16) is rated 3.9 out of 5 by 8.
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