Date published: 2025-9-8
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Ferrochelatase Antibody (A-3) - Western Blotting - Image 356864
铁螯合酶抗体 (A-3): sc-377377. NCI-H460 (A), HeLa (B), MCF7 (C) 和 NIH/3T3 (D) 全细胞裂解液中铁螯合酶表达的 Western 印迹分析.
铁螯合酶抗体 (A-3): sc-377377. NCI-H460 (A), HeLa (B), MCF7 (C) 和 NIH/3T3 (D) 全细胞裂解液中铁螯合酶表达的 Western 印迹分析.

Ferrochelatase 抗体 (A-3): sc-377377

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说明书
  • Ferrochelatase 抗体 A-3 是小鼠单克隆 IgG1 κ，Ferrochelatase抗体, 在22篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Ferrochelatase的C-terminus附近的氨基酸329-369之间
  • Ferrochelatase 抗体 (A-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 mitochondrial precursor and mature Ferrochelatase; 也和以下物种反应，包括： 和 equine and bovine
  • 抗Ferrochelatase抗体（A-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ferrochelatase (A-3): sc-377377的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Ferrochelatase 抗体 (A-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Ferrochelatase 抗体 (A-3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Ferrochelatase抗体(A-3)是一种IgG1 κ小鼠单克隆Ferrochelatase抗体（也被称为亚铁螯合酶抗体），它能够通过WB（蛋白质印迹）、IP（免疫沉淀）、IF（免疫荧光）、IHC(P)（免疫组织化学（石蜡））和ELISA（酶联免疫吸附试验）检测到来自小鼠、大鼠和人类样本中的Ferrochelatase蛋白。Ferrochelatase抗体(A-3)既有非偶联形式的抗Ferrochelatase抗体，也有多种偶联形式的抗Ferrochelatase抗体，包括琼脂糖、HRP（辣根过氧化物酶）、PE（藻红蛋白）、FITC（异硫氰酸荧光素）和多种Alexa Fluor®偶联物。Ferrochelatase，也被称为亚铁螯合酶或血红素合成酶，是原血红素生物合成的末端酶，催化亚铁形式的铁插入原卟啉IX中。成熟的Ferrochelatase是一种同源二聚体，与线粒体膜相关的蛋白质，它是在N端54个氨基酸转运肽的下游进行翻译的。Ferrochelatase含有两个对一氧化氮(NO)敏感的簇和协调的2FE-2S簇，这些簇可能潜在地作为一氧化氮传感器。编码Ferrochelatase酶的FECH基因的缺陷会导致红细胞生成性原卟啉病(EPP)，这是一种以光敏感性和肝胆疾病为特征的显性遗传性卟啉代谢疾病。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ferrochelatase 抗体 (A-3) 参考文献:

  1. 卟啉-底物与小鼠铁螯合酶的结合：对该酶热稳定性的影响。  |  Franco, R., et al. 2005. Biochem J. 386: 599-605. PMID: 15496139
  2. 来自显性遗传性红细胞生成性原卟啉症患者的由野生型和突变亚基组成的铁螯合酶是一种活跃但不稳定的二聚体。  |  Ohgari, Y., et al. 2005. Hum Mol Genet. 14: 327-34. PMID: 15574461
  3. 原卟啉型 Fech 小鼠的肝脏基因表达与胆汁淤积性损伤有关，但与血红素调节池的明显耗竭无关。  |  Davies, R., et al. 2005. Am J Pathol. 166: 1041-53. PMID: 15793285
  4. 红细胞原卟啉异二聚体铁螯合酶的生产和特征描述。  |  Najahi-Missaoui, W. and Dailey, HA. 2005. Blood. 106: 1098-104. PMID: 15831704
  5. 影响铁螯合酶基因启动子中 SP1 结合位点的点突变会影响基因转录并导致红细胞生成性原卟啉症。  |  Di Pierro, E., et al. 2005. Exp Hematol. 33: 584-91. PMID: 15850836
  6. Synechocystis sp. PCC 6803 的 CP47 突变体中的光系统 II 组装依赖于受铁螯合酶调节的叶绿素前体水平。  |  Sobotka, R., et al. 2005. J Biol Chem. 280: 31595-602. PMID: 16027152
  7. 正常的皮肤铁螯合酶活性并不能保护人体皮肤免受原卟啉诱导的光敏反应的伤害。  |  Elder, G. 2005. J Invest Dermatol. 125: 580; author reply 580-1. PMID: 16117803
  8. 铁螯合酶对过渡态抑制剂 N-甲基间卟啉的金属化：对催化反应机制的影响。  |  Shipovskov, S., et al. 2005. J Mol Biol. 352: 1081-90. PMID: 16140324
  9. 抑制铁螯合酶会损害血管 eNOS/NO 和 sGC/cGMP 信号传导。  |  Zhang, B., et al. 2018. PLoS One. 13: e0200307. PMID: 29985945
  10. 铁螯合酶缺乏会削弱铁螯合剂去铁胺对氨基乙酰丙酸介导的原卟啉 IX 的增强作用  |  Palasuberniam, P., et al. 2019. Photochem Photobiol. 95: 1052-1059. PMID: 30767226
  11. 铁螯合酶突变体小鼠视网膜表型显示原卟啉积累和新生血管反应减弱  |  Sardar Pasha, SPB., et al. 2021. Invest Ophthalmol Vis Sci. 62: 36. PMID: 33620374
  12. 造血细胞中铁螯合酶基因缺失导致的晚发性原卟啉症继发骨髓增生性疾病中的光敏感和急性肝损伤。  |  Goodwin, RG., et al. 2006. Blood. 107: 60-2. PMID: 16150949

订购信息

产品名称产品编号规格价格数量收藏夹

Ferrochelatase 抗体 (A-3)

sc-377377
200 µg/ml
RMB2377.00

Ferrochelatase (A-3): m-IgG Fc BP-HRP 套装

sc-530017
200 µg Ab; 10 µg BP
RMB2662.00

Ferrochelatase (A-3): m-IgGκ BP-HRP 套装

sc-523303
200 µg Ab, 40 µg BP
RMB2662.00

Ferrochelatase (A-3): m-IgG1 BP-HRP 套装

sc-543819
200 µg Ab; 20 µg BP
RMB2662.00

Ferrochelatase 抗体 (A-3) AC

sc-377377 AC
500 µg/ml, 25% agarose
RMB3129.00

Ferrochelatase 抗体 (A-3) HRP

sc-377377 HRP
200 µg/ml
RMB2377.00

Ferrochelatase 抗体 (A-3) FITC

sc-377377 FITC
200 µg/ml
RMB2482.00

Ferrochelatase 抗体 (A-3) PE

sc-377377 PE
200 µg/ml
RMB2580.00

Ferrochelatase 抗体 (A-3) Alexa Fluor® 488

sc-377377 AF488
200 µg/ml
RMB2685.00

Ferrochelatase 抗体 (A-3) Alexa Fluor® 546

sc-377377 AF546
200 µg/ml
RMB2685.00

Ferrochelatase 抗体 (A-3) Alexa Fluor® 594

sc-377377 AF594
200 µg/ml
RMB2685.00

Ferrochelatase 抗体 (A-3) Alexa Fluor® 647

sc-377377 AF647
200 µg/ml
RMB2685.00

Ferrochelatase 抗体 (A-3) Alexa Fluor® 680

sc-377377 AF680
200 µg/ml
RMB2685.00

Ferrochelatase 抗体 (A-3) Alexa Fluor® 790

sc-377377 AF790
200 µg/ml
RMB2685.00

Ferrochelatase (A-3) 中和勝肽

sc-377377 P
100 µg/0.5 ml
RMB511.00

how can i get the free trial of an antibody?

Asked by: Floy
Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-14

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2017-02-03
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Rated 5 out of 5 by from Strong and cleanFantastic antibody, very strong bands. Specific and clear
Date published: 2023-01-25
Rated 5 out of 5 by from works by western blotworks by WB in human hepatoma and keratinocyte cell lines
Date published: 2018-04-03
Rated 5 out of 5 by from Strong bandStrong band at the right size, I need a expression plasmid or KO to further confirm though.
Date published: 2017-06-14
Rated 5 out of 5 by from great antibodyThis is a great antibody with a sharp and clear band.
Date published: 2017-03-16
Rated 5 out of 5 by from Great Ferrochelatase detectionStrong, reliable antibody for Ferrochelatase detection in several species. Tested in multiple cell lines with consistent, reliable banding in Western blot.
Date published: 2017-02-03
Rated 5 out of 5 by from used in Western to verify KDused in Western to verify KD. Did not have any issues.
Date published: 2015-10-23
Rated 5 out of 5 by from GreatGreat, strong band in WB in Jurkat whole cell lysate. -SCBT QC
Date published: 2015-06-27
Rated 5 out of 5 by from Performs well in WesternPerforms well in Western, good signal with K-562 and HEL 92.1.7 whole cell lysates. -SCBT QC
Date published: 2015-03-31
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Ferrochelatase 抗体 (A-3) is rated 5.0 out of 5 by 9.
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