Date published: 2025-9-7
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ferritin light chain Antibody (D-9) - Western Blotting - Image 289702
铁蛋白轻链抗体 (D-9): sc-74513. 人脾组织提取物中铁蛋白轻链表达的 Western 印迹分析.
铁蛋白轻链抗体 (D-9): sc-74513. 人脾组织提取物中铁蛋白轻链表达的 Western 印迹分析.

ferritin light chain 抗体 (D-9): sc-74513

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说明书
  • ferritin light chain 抗体 D-9 是小鼠单克隆 IgG2a κ，ferritin light chain抗体, 在38篇文献中引用，规格为200 µg/ml
  • 免疫human物种的ferritin light chain的氨基酸131-175
  • ferritin light chain 抗体 (D-9) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 ferritin light chain
  • 抗ferritin light chain抗体（D-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ferritin light chain (D-9): sc-74513的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 ferritin light chain 抗体 (D-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ferritin light chain 抗体 (D-9) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Ferritin 抗体 包括 ferritin heavy chain, ferritin light chain和mitochondrial ferritin

铁蛋白轻链抗体（D-9）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测人类铁蛋白轻链。抗铁蛋白轻链抗体（D-9）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。铁蛋白轻链在铁代谢中起着至关重要的作用，特别是在细胞内铁的储存和释放方面，这对于维持细胞内铁的平衡和防止氧化损伤至关重要。铁蛋白轻链与其对应的重链形成中空球状结构，可以以无毒的形式隔离多达4500个铁原子，从而起到缓冲铁过载的作用。铁蛋白轻链表达失调与多种病理状况有关，包括神经退行性疾病和白内障的形成，这凸显了其在健康和疾病中的重要性。编码人类铁蛋白轻链的基因位于19q13.3-q13.4染色体上，其表达受到细胞铁含量的严格调控，这使得铁蛋白轻链成为人体应对缺铁和铁超负荷的关键因素。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ferritin light chain 抗体 (D-9) 参考文献:

  1. 晶状体中铁蛋白轻链 mRNA 含量高。  |  Cheng, Q., et al. 2000. Biochem Biophys Res Commun. 270: 349-55. PMID: 10753629
  2. 重链铁蛋白可增强丝氨酸羟甲基转移酶的表达和胸苷的新生物合成。  |  Oppenheim, EW., et al. 2001. J Biol Chem. 276: 19855-61. PMID: 11278996
  3. 硫化物是哺乳动物铁蛋白的一种高效铁释放剂。  |  Cassanelli, S. and Moulis, J. 2001. Biochim Biophys Acta. 1547: 174-82. PMID: 11343803
  4. 铁蛋白轻链与 PEN-2 相互作用并影响γ-分泌酶的活性。  |  Li, X., et al. 2013. Neurosci Lett. 548: 90-4. PMID: 23685131
  5. 铁蛋白轻链亚基：蛋白质笼电子转移的关键要素。  |  Carmona, U., et al. 2014. Chem Commun (Camb). 50: 15358-61. PMID: 25348725
  6. 铁蛋白轻链可保护免受败血症引起的炎症和器官损伤。  |  Zarjou, A., et al. 2019. Front Immunol. 10: 131. PMID: 30804939
  7. 缺氧诱导的铁蛋白轻链（FTL）促进上皮间质转化和胶质瘤的耐药性。  |  Liu, J., et al. 2020. J Exp Clin Cancer Res. 39: 137. PMID: 32677981
  8. 铁蛋白轻链缺乏引起的铁死亡通过干扰子宫螺旋动脉重塑，参与先兆子痫的病理生理。  |  Yang, X., et al. 2022. Redox Biol. 58: 102555. PMID: 36446230
  9. 铁蛋白轻链促进胶质瘤免疫微环境的重新编程，并促进胶质瘤的进展。  |  Li, H., et al. 2023. Theranostics. 13: 3794-3813. PMID: 37441589
  10. 铁蛋白轻链的过度表达是乳腺癌的预后不良因素。  |  Tang, C., et al. 2023. Mol Biol Rep. 50: 8097-8109. PMID: 37542685
  11. 将人类铁蛋白基因分配到 11 号染色体和 19q13.3----19qter.  |  Worwood, M., et al. 1985. Hum Genet. 69: 371-4. PMID: 3857215
  12. 高分辨率下重组人 H 铁蛋白和马 L 铁蛋白三维结构的比较。  |  Hempstead, PD., et al. 1997. J Mol Biol. 268: 424-48. PMID: 9159481

订购信息

产品名称产品编号规格价格数量收藏夹

ferritin light chain 抗体 (D-9)

sc-74513
200 µg/ml
RMB2377.00

ferritin light chain (D-9): m-IgG Fc BP-HRP 套装

sc-525725
200 µg Ab; 10 µg BP
RMB2662.00

ferritin light chain (D-9): m-IgG2a BP-HRP 套装

sc-546382
200 µg Ab; 10 µg BP
RMB2662.00

ferritin light chain 抗体 (D-9) AC

sc-74513 AC
500 µg/ml, 25% agarose
RMB3129.00

ferritin light chain 抗体 (D-9) HRP

sc-74513 HRP
200 µg/ml
RMB2377.00

ferritin light chain 抗体 (D-9) FITC

sc-74513 FITC
200 µg/ml
RMB2482.00

ferritin light chain 抗体 (D-9) PE

sc-74513 PE
200 µg/ml
RMB2580.00

ferritin light chain 抗体 (D-9) Alexa Fluor® 488

sc-74513 AF488
200 µg/ml
RMB2685.00

ferritin light chain 抗体 (D-9) Alexa Fluor® 546

sc-74513 AF546
200 µg/ml
RMB2685.00

ferritin light chain 抗体 (D-9) Alexa Fluor® 594

sc-74513 AF594
200 µg/ml
RMB2685.00

ferritin light chain 抗体 (D-9) Alexa Fluor® 647

sc-74513 AF647
200 µg/ml
RMB2685.00

ferritin light chain 抗体 (D-9) Alexa Fluor® 680

sc-74513 AF680
200 µg/ml
RMB2685.00

ferritin light chain 抗体 (D-9) Alexa Fluor® 790

sc-74513 AF790
200 µg/ml
RMB2685.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-04-13
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Rated 2 out of 5 by from Detects 2 bandsI have used this antibody at a dilution of 1:500 (as per the data sheet). I get 2 crisp bands, one just below 25kDa and one slightly above 15 kDa (approx 19 kDa). I am confused which one to quantify? Can someone help me with this?
Date published: 2022-09-16
Rated 3 out of 5 by from Not bad for western blotI used this antibody in western blot of human microglial cell. I obtained two bands; one between 30-37KDa and a second one below 20KDa. Anyway, the bands are clear and there is no background.
Date published: 2021-11-23
Rated 5 out of 5 by from Ferritin antibodyThe ferritin antibody (sc-74513) was working well in our Western blotting.
Date published: 2021-03-05
Rated 5 out of 5 by from perfect performanceThis antibody recognizes a single band in SiHa cell line (19 kDa) by western blot experiments performed using total cellular extract (50 ug). This band increases after treatment of cells with iron and the antibody (dil 1:500) always shows perfect performance.
Date published: 2021-01-26
Rated 4 out of 5 by from FTL1The FTL1 Antibody works very well for Western blot applications, showing strong and clear banding with minimal background noise present
Date published: 2017-07-31
Rated 4 out of 5 by from good antibodyWorks well for WB. Tested at 1:500. Happy with the antibody.
Date published: 2017-03-07
Rated 5 out of 5 by from excellent for WBexcellent for WB with correct size band and right expression pattern by human samples with no other none specific bands
Date published: 2016-12-30
Rated 5 out of 5 by from Specific antibodyThe (D-9) clone is specific for ferritin light chain, and does not cross react with ferritin heavy chain. A popular choice for WB and IF as supported in numerous product citations.
Date published: 2016-12-19
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ferritin light chain 抗体 (D-9) is rated 4.2 out of 5 by 12.
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