Date published: 2025-9-7
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FcRn Antibody (B-8) - Western Blotting - Image 377303
FcRn 抗体 (B-8): sc-271745. COLO 205 (A), RD (B), AN3 CA (C) 和 HEL 92. 1. 7 (D) 全细胞裂解液中 FcRn 表达的 Western 印迹分析.
FcRn 抗体 (B-8): sc-271745. COLO 205 (A), RD (B), AN3 CA (C) 和 HEL 92. 1. 7 (D) 全细胞裂解液中 FcRn 表达的 Western 印迹分析.

FcRn 抗体 (B-8): sc-271745

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说明书
  • FcRn 抗体 B-8 是小鼠单克隆 IgG2a κ，FcRn抗体, 在21篇文献中引用，规格为200 µg/ml
  • 针对FcRn的human起源的N-terminal细胞外域内的氨基酸24-297
  • FcRn 抗体 (B-8) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 FcRn
  • 抗FcRn抗体（B-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取FcRn (B-8): sc-271745的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 FcRn 抗体 (B-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与FcRn 抗体 (B-8) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

FcRn抗体（B-8）是一种IgG2a κ小鼠单克隆FcRn抗体（也称为FCGRT抗体、IgG受体的Fc片段和转运蛋白抗体、45 kDa跨膜糖蛋白MHC1同源抗体，或与β-2-微球蛋白相关的MHC I类同源抗体），可通过WB、IP、IF、IHC(P)和ELISA检测人类的FcRn蛋白。FcRn抗体（B-8）既可作为非偶联的抗FcRn抗体形式提供，也可作为多种偶联的抗FcRn抗体形式提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。存在于新生小鼠和大鼠肠上皮中的FcRN介导母乳中免疫球蛋白G（IgG）的选择性摄取，从而帮助新生动物获得被动免疫。FcRn（也称为FCGRT、Brambell受体、FcRn α链、IgG Gc受体和新生儿Fc受体）由重链和β-2-微球蛋白组成。FcRn重链与MHC I类分子的氨基酸同一性约为35%。FcRN定位于血管内皮细胞的内体中，并选择性地将IgG回收至细胞表面，从而保护IgG免受溶酶体分解代谢。这种保护机制是确保IgG成为所有血浆蛋白中寿命最长的主要成分。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

FcRn 抗体 (B-8) 参考文献:

  1. 对人类IgG1上Fc gamma RI、Fc gamma RII、Fc gamma RIII和FcRn的结合位点进行高分辨率测绘，并设计出与Fc gamma R结合能力更强的IgG1变体  |  Shields, RL., et al. 2001. J Biol Chem. 276: 6591-604. PMID: 11096108
  2. 人 FcRn 在 Madin-Darby 犬肾细胞中的功能重建需要联合表达人 beta 2-微球蛋白。  |  Claypool, SM., et al. 2002. J Biol Chem. 277: 28038-50. PMID: 12023961
  3. 膜锚定的人类 FcRn 在没有 IgG 的情况下也能寡聚。  |  Praetor, A., et al. 2002. J Mol Biol. 321: 277-84. PMID: 12144784
  4. 在 MDCK 细胞中表达的 FcRn 的 IgG 转运和再循环揭示了配体诱导的再分布。  |  Detmer, SA., et al. 2002. EMBO J. 21: 5953. PMID: 12411512
  5. 产生人类形式的 MHC I 类相关受体 FcRn 的突变变体，其对小鼠免疫球蛋白 G 的亲和力增强。  |  Zhou, J., et al. 2003. J Mol Biol. 332: 901-13. PMID: 12972260
  6. 通过 MHC I 类相关受体 FcRn 观察 IgG 挽救的部位和动态。  |  Ober, RJ., et al. 2004. J Immunol. 172: 2021-9. PMID: 14764666
  7. 受体 FcRn 介导的 IgG 外吞：单分子水平分析。  |  Ober, RJ., et al. 2004. Proc Natl Acad Sci U S A. 101: 11076-81. PMID: 15258288
  8. 通过连续几轮定点突变，将小鼠 MHC I 类相关受体 FcRn 的结合特性赋予人类同源物。  |  Zhou, J., et al. 2005. J Mol Biol. 345: 1071-81. PMID: 15644205
  9. 抗 FcRn 抗体 4C9 在大鼠体内的药代动力学效应：FcRn 抑制剂用于治疗体液自身免疫和同种免疫疾病的意义。  |  Getman, KE. and Balthasar, JP. 2005. J Pharm Sci. 94: 718-29. PMID: 15682382
  10. 从分拣内体到外吞：Rab4 和 Rab11 GTP 酶在循环过程中与 Fc 受体 FcRn 的缔合作用。  |  Ward, ES., et al. 2005. Mol Biol Cell. 16: 2028-38. PMID: 15689494
  11. 生成高亲和力抗人FcRn抗体罗扎诺利珠单抗并对其进行表征，以及不同分子形式对降低血浆IgG浓度的影响  |  Smith, B., et al. 2018. MAbs. 10: 1111-1130. PMID: 30130439
  12. 新型FcRn拮抗剂efgartigimod在原发性免疫性血小板减少症成人患者中的2期研究。  |  Newland, AC., et al. 2020. Am J Hematol. 95: 178-187. PMID: 31821591

订购信息

产品名称产品编号规格价格数量收藏夹

FcRn 抗体 (B-8)

sc-271745
200 µg/ml
RMB2377.00

FcRn (B-8): m-IgGκ BP-HRP 套装

sc-522048
200 µg Ab, 40 µg BP
RMB2662.00

FcRn (B-8): m-IgG2a BP-HRP 套装

sc-546107
200 µg Ab; 10 µg BP
RMB2662.00

FcRn 抗体 (B-8) AC

sc-271745 AC
500 µg/ml, 25% agarose
RMB3129.00

FcRn 抗体 (B-8) HRP

sc-271745 HRP
200 µg/ml
RMB2377.00

FcRn 抗体 (B-8) FITC

sc-271745 FITC
200 µg/ml
RMB2482.00

FcRn 抗体 (B-8) PE

sc-271745 PE
200 µg/ml
RMB2580.00

FcRn 抗体 (B-8) Alexa Fluor® 488

sc-271745 AF488
200 µg/ml
RMB2685.00

FcRn 抗体 (B-8) Alexa Fluor® 546

sc-271745 AF546
200 µg/ml
RMB2685.00

FcRn 抗体 (B-8) Alexa Fluor® 594

sc-271745 AF594
200 µg/ml
RMB2685.00

FcRn 抗体 (B-8) Alexa Fluor® 647

sc-271745 AF647
200 µg/ml
RMB2685.00

FcRn 抗体 (B-8) Alexa Fluor® 680

sc-271745 AF680
200 µg/ml
RMB2685.00

FcRn 抗体 (B-8) Alexa Fluor® 790

sc-271745 AF790
200 µg/ml
RMB2685.00

Does conjugated antibody work for flow cytometry? answer SC provided in past. "The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!"

Asked by: Gav1234
Thank you for your question. We recommend using FITC or PE for flow cytometry.
Answered by: BlakeJ
Date published: 2024-08-14

Does this antibody block FcRn-mediated internalization of human IgGs in cell lines? If so, what concentration should be used to block the internalization?

Asked by: DanielD
Thank you for your question. We do not have any reason to believe that this antibody would block FcRn-mediated internalization. However, we have not tested for this or conducted any research to to support one way or another.
Answered by: BlakeJ
Date published: 2024-03-28

Does this antibody have reactivity to Mouse FcRn(Fcgrt)?? If so, Can I use fluorescence conjugated antibody for flow cytometry??

Asked by: kunjoo2
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Is it specific for any particular type of FcRN, like FcgR1, II or III? Or we measure total FcRn with this antibody?

Asked by: AnnaCC
Thank you for your question. This monoclonal antibody is raised against amino acids 24-297 mapping within an N-terminal extracellular domain of FcRn of human origin. The exact epitope recognized by this monoclonal antibody has not been determined. The human gene symbol is FCGRT and the protein accession number for the antigen is P55899.
Answered by: Technical Support
Date published: 2020-03-19

Is FCRN antibody conjugated with FITC suitable for flow cytometry? If yes, what's the recommended dilution? Thanks.

Asked by: Cordero
The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates! We have not yet tested this antibody for flow cytometry. Please check our protocol for our general recommendations for how much antibody to use.
Answered by: Technical Support
Date published: 2019-02-23

Can I use this mouse monoclonal antibody for blocking the FcRn receptor in human cell lines? I pretend block the FcRn receptor during the cell culture (functional studies/inhibition/blocking receptors).

Asked by: Cátia Rocha
Thank you for your question. The FcRn Antibody (B-8): sc-271745 is recommended for detection of FcRn of human origin by WB, IP, IF, IHC(P) and ELISA, but we have no data indicating whether it also blocks the receptor’s function. You can order a free sample of this antibody with your next antibody purchase; we would appreciate your insight.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-30

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue? Or even double or triple staining on the same samples?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-271745 PE), fluorescein (sc-271745 FITC), Alexa Fluor® 488 (sc-271745 AF488) or Alexa Fluor® 647 (sc-271745 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 2
Date published: 2017-01-10
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Rated 4 out of 5 by from Specific, intense bandsHighly specific bands with just a little bit of background.
Date published: 2020-07-03
Rated 5 out of 5 by from Outstanding !!Much better product than polyclonal alternatives for multiple applications
Date published: 2017-01-09
Rated 5 out of 5 by from Reliable Antibody for Many ApplicationsThis antibody is well-cited for many different common applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal in each application.
Date published: 2016-12-19
Rated 5 out of 5 by from This antibody works well in IF in transfectedThis antibody works well in IF in transfected human cell lines.
Date published: 2015-10-12
Rated 5 out of 5 by from Crisp band seen in WB using human skeletalCrisp band seen in WB using human skeletal muscle tissue extract. -SCBT QC
Date published: 2015-06-20
Rated 5 out of 5 by from Antibody nonAntibody non-specifically binds to human IgG by western blot.
Date published: 2015-03-25
Rated 5 out of 5 by from Publishable data with dendritic cellPublishable data with dendritic cell - SCBT Publication Review
Date published: 2015-03-25
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FcRn 抗体 (B-8) is rated 4.9 out of 5 by 7.
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