Date published: 2025-9-8
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Fascin 1 Antibody (55K-2) - Western Blotting - Image 354642
Fascin 1 抗体 (55K-2): sc-21743. NIH/3T3 (A), SH-SY5Y (B), Hep G2 (C) 和 U-251-MG (D) 全细胞裂解液中 Fascin 1 表达的 Western 印迹分析.
Fascin 1 抗体 (55K-2): sc-21743. NIH/3T3 (A), SH-SY5Y (B), Hep G2 (C) 和 U-251-MG (D) 全细胞裂解液中 Fascin 1 表达的 Western 印迹分析.

Fascin 1 抗体 (55K-2): sc-21743

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说明书
  • Fascin 1抗体(55K-2)是小鼠单克隆IgG1 κ, 在60篇文献中引用，规格为200 µg/ml
  • 针对Fascin纯化的human来源Hela细胞
  • 抗-Fascin 1 抗体 (55K-2) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 Fascin 1
  • 抗Fascin 1抗体（55K-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Fascin 1 (55K-2): sc-21743的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Fascin 1 抗体 (55K-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Fascin 1 抗体 (55K-2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Fascin 抗体 包括 Fascin 2

Fascin 1 抗体（55K-2）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学等方法检测小鼠、大鼠和人类样本中的 Fascin 1。抗Fascin 1抗体（55K-2）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Fascin 1在组织肌动蛋白细胞骨架方面发挥着关键作用，特别是在形成细胞粘附和迁移所必需的F-肌动蛋白微刺方面。这些微刺在粘附到细胞外基质糖蛋白血栓素原1上时形成，对于组织重塑和伤口修复等细胞过程至关重要。通过捆绑肌动蛋白微丝，Fascin 1有助于维持动态细胞结构（包括微刺、应力纤维和膜皱褶）的完整性。Fascin 1已被确定为卵巢上皮异常病理的潜在预后因子，也是卵巢癌治疗的新靶点。Fascin 1能够识别血液和组织中的树突状细胞，这进一步凸显了其在免疫反应和细胞动力学中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Fascin 1 抗体 (55K-2) 参考文献:

  1. Fascin 和儿童组织细胞病变的鉴别诊断。  |  Jaffe, R., et al. 1998. Pediatr Dev Pathol. 1: 216-21. PMID: 10463281
  2. 位于 17q25 的人类视网膜筋膜蛋白基因（FSCN2）的特征：筋膜蛋白基因与细胞质肌动蛋白基因的密切物理联系。  |  Tubb, BE., et al. 2000. Genomics. 65: 146-56. PMID: 10783262
  3. 血栓松蛋白-1 对 fascin 棘突的刺激是由 GTP 酶 Rac 和 Cdc42 介导的。  |  Adams, JC. and Schwartz, MA. 2000. J Cell Biol. 150: 807-22. PMID: 10953005
  4. Fascin是一种与细胞运动性相关的肌动蛋白缔合作用蛋白，在激素受体阴性乳腺癌中上调。  |  Grothey, A., et al. 2000. Br J Cancer. 83: 870-3. PMID: 10970687
  5. 卵巢癌细胞培养物, 边缘性卵巢肿瘤和癌变卵巢肿瘤中运动相关蛋白 fascin 的表达增加。  |  Hu, W., et al. 2000. Clin Exp Metastasis. 18: 83-8. PMID: 11206843
  6. 作为 ACE2 辅助蛋白的肌动蛋白束蛋白 Fascin-1  |  Ogunlade, B., et al. 2022. Cell Mol Neurobiol. 42: 255-263. PMID: 32865675
  7. Fascin-1在胃肠道癌症的发病机理、诊断和治疗中的新兴作用。  |  Ristic, B., et al. 2021. Cancers (Basel). 13: PMID: 34064154
  8. Fascin-1与单发纤维瘤/血管组织细胞瘤的复发有关。  |  Yamamoto, Y., et al. 2021. Mol Clin Oncol. 15: 199. PMID: 34462655
  9. Fascin-1在呼吸系统相关癌症的发病机理、诊断和管理中的作用。  |  Zhang, N., et al. 2022. Front Oncol. 12: 948110. PMID: 36033434
  10. Fascin-1：癌症生物学中的最新生物学功能和治疗意义。  |  Li, CH., et al. 2022. BBA Adv. 2: 100052. PMID: 37082587
  11. Fascin-1表达与神经内分泌前列腺癌有关，并直接受雄激素受体抑制。  |  Turpin, A., et al. 2023. Br J Cancer. 129: 1903-1914. PMID: 37875732
  12. Fascin-1限制小胶质细胞中的肌球蛋白活性，以控制受伤脊髓的机械特性。  |  Huang, J., et al. 2024. J Neuroinflammation. 21: 88. PMID: 38600569

订购信息

产品名称产品编号规格价格数量收藏夹

Fascin 1 抗体 (55K-2)

sc-21743
200 µg/ml
RMB2377.00

Fascin 1 (55K-2): m-IgG Fc BP-HRP 套装

sc-526607
200 µg Ab; 10 µg BP
RMB2662.00

Fascin 1 (55K-2): m-IgG1 BP-HRP 套装

sc-531980
200 µg Ab; 20 µg BP
RMB2662.00

Fascin 1 抗体 (55K-2) AC

sc-21743 AC
500 µg/ml, 25% agarose
RMB3129.00

Fascin 1 抗体 (55K-2) HRP

sc-21743 HRP
200 µg/ml
RMB2377.00

Fascin 1 抗体 (55K-2) FITC

sc-21743 FITC
200 µg/ml
RMB2482.00

Fascin 1 抗体 (55K-2) PE

sc-21743 PE
200 µg/ml
RMB2580.00

Fascin 1 抗体 (55K-2) Alexa Fluor® 488

sc-21743 AF488
200 µg/ml
RMB2685.00

Fascin 1 抗体 (55K-2) Alexa Fluor® 546

sc-21743 AF546
200 µg/ml
RMB2685.00

Fascin 1 抗体 (55K-2) Alexa Fluor® 594

sc-21743 AF594
200 µg/ml
RMB2685.00

Fascin 1 抗体 (55K-2) Alexa Fluor® 647

sc-21743 AF647
200 µg/ml
RMB2685.00

Fascin 1 抗体 (55K-2) Alexa Fluor® 680

sc-21743 AF680
200 µg/ml
RMB2685.00

Fascin 1 抗体 (55K-2) Alexa Fluor® 790

sc-21743 AF790
200 µg/ml
RMB2685.00

can this antibody be used for IF to tissue of rat?

Asked by: Longyu
Thank you for your question. Fascin 1 (55K-2) can be used for IF for mouse, rat and human samples.
Answered by: BlakeJ
Date published: 2025-09-01

Has the product been validated in mouse flow cytometry?

Asked by: jingpu
Thank you for your question. Anti-Fascin 1 Antibody (55K-2) is recommended for detection of Fascin 1 of mouse, rat and human origin by WB, IP, IF and IHC(P). It has not been validated by flow cytometry.
Answered by: Technical Service SH
Date published: 2025-08-08

Is it possible to have this antibody formulated without any gelatin? We would like to conjugate it to metal tags of Imaging Mass Cytometry, but are worried the 0.1% gelatin might affect the conjugation efficiency.

Asked by: Maros
Thank you for your question. To request a gelatin free antibody please email [email protected].
Answered by: BlakeJ
Date published: 2023-07-11

Can the conjugated Fascin 1 Antibody (55K-2): sc-21743 be used for flow cytometry?

Asked by: Milsee
Thank you for your question. We recommend using a FITC or PE for Flow Cytometry. Here is our recommended protocol : https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: BlakeJ
Date published: 2024-03-28

Can you recommend a positive control to use with Fascin 1 (55K-2): sc-21743 monoclonal antibody for Western blot?

Asked by: jenni
We recommend NIH/3T3: sc-2210, SH-SY5Y: sc-3812 or Hep G2: sc-2227 whole cell lysates to use as positive controls as we have great Western blot data showing specific detection of Fascin 1 in these cell lines.
Answered by: Technical Support 15
Date published: 2017-01-10
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Rated 5 out of 5 by from Great for multiple applications !!Strong WB,IF and IHC; far better product than polyclonal alternatives
Date published: 2017-01-14
Rated 5 out of 5 by from Good WB dataThe Fascin 1 Antibody (55K-2): sc-21743 shows strong bands with minimal background in Western blot applications. This antibody has been cited 20 times for further verification.
Date published: 2017-01-10
Rated 5 out of 5 by from Used in IHC and worked well in mouse cellsUsed in IHC and worked well in mouse cells.
Date published: 2015-07-29
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmicProduced excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human esophagus tissue. -SCBT QC
Date published: 2015-04-14
Rated 5 out of 5 by from Published immunoperoxidase staining of humanPublished immunoperoxidase staining of human cell lines: OC3, SCC4, and OEC-M1. -SCBT Publication Review
Date published: 2015-04-02
Rated 5 out of 5 by from Used for IF with myeloid dendritic cells (DCs)Used for IF with myeloid dendritic cells (DCs). -SCBT Publication Review
Date published: 2015-04-02
Rated 5 out of 5 by from Published western blot data using A549Published western blot data using A549, DCs, 12Z cells and tumor cells. -SCBT Publication Review
Date published: 2015-02-16
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Fascin 1 抗体 (55K-2) is rated 5.0 out of 5 by 7.
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