Date published: 2025-9-8
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FAS-L Antibody (NOK-1) - Western Blotting - Image 6227
FAS-L 抗体 (NOK-1): sc-19681. 用 FAS-L 抗体 (NOK-1): sc-19681 免疫沉淀并用 FAS-L (C-178): sc-6237 检测 K-562 (A) 和 HL-60 (B) 全细胞裂解液中 FAS-L 表达的 Western 印迹分析.
FAS-L 抗体 (NOK-1): sc-19681. 用 FAS-L 抗体 (NOK-1): sc-19681 免疫沉淀并用 FAS-L (C-178): sc-6237 检测 K-562 (A) 和 HL-60 (B) 全细胞裂解液中 FAS-L 表达的 Western 印迹分析.

FAS-L 抗体 (NOK-1): sc-19681

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说明书
  • FAS-L 抗体 NOK-1 是小鼠单克隆 IgG1 κ，FAS-L抗体, 在37篇文献中引用，规格为200 µg/ml
  • 针对表达重组human FAS-L的L5178Y小鼠T淋巴细胞
  • FAS-L 抗体 (NOK-1) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 FAS-L; 不建议用于WB
  • 抗FAS-L抗体（NOK-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还提供无叠氮化物中和剂，用于中和 FAS-L 的细胞毒性作用，sc-19681 L，200 µg/0.1 ml
  • 关于如何获取FAS-L (NOK-1): sc-19681的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 FAS-L 抗体 (NOK-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与FAS-L 抗体 (NOK-1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

FAS-L抗体（NOK-1）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹、免疫沉淀、免疫荧光、石蜡包埋切片免疫组织化学和流式细胞术检测小鼠、大鼠和人类样本中的FAS-L蛋白（也称为FASLG、CD178、CD95-L、TNFSF6或Fas配体）。FAS-L抗体（NOK-1）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合物。细胞毒性T淋巴细胞（CTL）介导的细胞毒性是免疫监视病毒感染或转化细胞特异性效应机制的重要组成部分。有两种机制可以解释这种活性，一种是穿孔素机制。另一种是基于FAS的机制，涉及转导分子FAS（也称为Apo-1）和FAS-L。人类FAS蛋白是一种细胞表面糖蛋白，属于CD40、神经生长因子受体和肿瘤坏死因子受体等受体家族。FAS抗原在多种淋巴细胞系中表达，其中某些细胞系在抗FAS抗体治疗后发生凋亡。这些发现有力地表明，靶向细胞死亡可能通过FAS与FAS-L或效应子的细胞间相互作用介导，并且FAS可能与CTL介导的细胞毒性密切相关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

FAS-L 抗体 (NOK-1) 参考文献:

  1. 人体小胶质细胞上的 Fas-配体（Fas-L）调节：体外研究。  |  Frigerio, S., et al. 2000. J Neuroimmunol. 105: 109-14. PMID: 10742551
  2. 人类结直肠肿瘤中涉及 Fas 和 Fas-L 分子的肿瘤逃逸机制。  |  Radfar, S., et al. 2000. Gastroenterol Clin Biol. 24: 1191-6. PMID: 11173732
  3. 高度恶性骨髓瘤浆细胞的 Fas-L 上调：在贫血和疾病进展的发病机制中的作用。  |  Silvestris, F., et al. 2001. Blood. 97: 1155-64. PMID: 11222356
  4. 蛋白激酶C-theta在Fas/Fas配体介导的细胞凋亡中的关键作用。  |  Manicassamy, S. and Sun, Z. 2007. J Immunol. 178: 312-9. PMID: 17182568
  5. 暴露于大利什曼原虫后，FasL 和 TRAIL 会诱导表皮凋亡和皮肤溃疡。  |  Eidsmo, L., et al. 2007. Am J Pathol. 170: 227-39. PMID: 17200196
  6. [慢性胃炎和十二指肠溃疡患儿胃窦粘膜中胃泌素, 体生长抑素, PCNA和Fas-L的表达]。  |  Xie, XZ., et al. 2006. Zhonghua Er Ke Za Zhi. 44: 774-7. PMID: 17229384
  7. 细胞凋亡介质 Fas 和 FasL 预测多发性硬化症 10 年内的残疾进展。  |  Lopatinskaya, L., et al. 2006. Mult Scler. 12: 704-9. PMID: 17262997
  8. 通过FasL蛋白转移消耗瘤内CD4+CD25+调节性T细胞可增强收养性T细胞转移的疗效。  |  Chen, A., et al. 2007. Cancer Res. 67: 1291-8. PMID: 17283166
  9. [在肉样瘤病和对照组中，吸烟者肺泡淋巴细胞的凋亡比不吸烟者更频繁]。  |  Kopiński, P., et al. 2006. Przegl Lek. 63: 841-7. PMID: 17288168
  10. 淋巴细胞介导的细胞毒性机制。  |  Henkart, PA. 1985. Annu Rev Immunol. 3: 31-58. PMID: 3904772
  11. Fas 及其配体在 T 细胞介导的细胞毒性的一般机制中的作用。  |  Hanabuchi, S., et al. 1994. Proc Natl Acad Sci U S A. 91: 4930-4. PMID: 7515183
  12. Fas 蛋白在正常小鼠的 CD4+CD8+ 胸腺细胞和活化的成熟淋巴细胞上高水平表达，但在狼疮易感株 MRL lpr/lpr 中却没有表达。  |  Drappa, J., et al. 1993. Proc Natl Acad Sci U S A. 90: 10340-4. PMID: 7694292

订购信息

产品名称产品编号规格价格数量收藏夹

FAS-L 抗体 (NOK-1)

sc-19681
200 µg/ml
RMB2377.00

FAS-L (NOK-1): m-IgG Fc BP-HRP 套装

sc-526589
200 µg Ab; 10 µg BP
RMB2662.00

FAS-L (NOK-1): m-IgG1 BP-HRP 套装

sc-531962
200 µg Ab; 20 µg BP
RMB2662.00

FAS-L 抗体 (NOK-1) L

sc-19681 L
200 µg/0.1 ml
RMB2377.00

FAS-L 抗体 (NOK-1) AC

sc-19681 AC
500 µg/ml, 25% agarose
RMB3129.00

FAS-L 抗体 (NOK-1) HRP

sc-19681 HRP
200 µg/ml
RMB2377.00

FAS-L 抗体 (NOK-1) FITC

sc-19681 FITC
200 µg/ml
RMB2482.00

FAS-L 抗体 (NOK-1) PE

sc-19681 PE
200 µg/ml
RMB2580.00

FAS-L 抗体 (NOK-1) Alexa Fluor® 488

sc-19681 AF488
200 µg/ml
RMB2685.00

FAS-L 抗体 (NOK-1) Alexa Fluor® 546

sc-19681 AF546
200 µg/ml
RMB2685.00

FAS-L 抗体 (NOK-1) Alexa Fluor® 594

sc-19681 AF594
200 µg/ml
RMB2685.00

FAS-L 抗体 (NOK-1) Alexa Fluor® 647

sc-19681 AF647
200 µg/ml
RMB2685.00

FAS-L 抗体 (NOK-1) Alexa Fluor® 680

sc-19681 AF680
200 µg/ml
RMB2685.00

FAS-L 抗体 (NOK-1) Alexa Fluor® 790

sc-19681 AF790
200 µg/ml
RMB2685.00

I am looking to utilize this antibody to neutralize FAS-L. Do I need to specify that I need it azide-free?

Asked by: Jame Dolan
Thank you for your question. To request an azide-free antibody please contact our special orders department for a price quote and lead time. Please email [email protected]
Answered by: BlakeJ
Date published: 2023-05-18

This particular antibody is listed as not for use with western blot, but the majority of the reviews are for western blots including a review from your QC department. What's really going on with this antibody?

Asked by: Allyson
Thank you for your question. We do not recommend this mouse monoclonal antibody, FAS-L (NOK-1): sc-19681, for use in Western blotting. Based on our in-house testing and data on the website the antibody is recommended for IP, IF, IHC(P) and FCM. We will remove the review from our QC department which mistakenly interpreted our published IP data with the antibody as WB data (because the WB was performed with a different antibody).
Answered by: Technical Support
Date published: 2017-11-22

Hello, out of Fas-Ligand antibodies N-20, C-178, Q-20, and C-20, only C-20 has given me consistent results in western blotting. NOK-1 antibody, the suggested replacement for C-20, is not listed for western blotting. Which Fas-L antibody should I use?

Asked by: Nayan
Thank you for your question. The NOK-1 clone is a well established antibody against FAS-L that is commonly used for flow cytometry (FCM), IF, IHC(P) and immunoprecipitation. It is also available azide-free for neutralizing the cytotoxic effects of FAS-L. This clone is not recommended for Western blotting. We do offer alternative clones that are specifically recommended for Western blotting. For human samples, we recommend, FAS-L Antibody (2.1): sc-33716. And for mouse samples we recommend, FAS-L Antibody (Kay-10): sc-19988. Links to these product pages are provided below. https://www.scbt.com/scbt/product/fas-l-antibody-2-1 https://www.scbt.com/scbt/product/fas-l-antibody-kay-10
Answered by: Technical Support
Date published: 2017-03-13

Can FAS-L (NOK-1): sc-19681 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, FAS-L (NOK-1): sc-19681 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Quite good antibody!1:200 diluted for wb of rat protein ,the band is strong and specia!
Date published: 2017-12-30
Rated 5 out of 5 by from WBI have used this FAS-L antibody for many times to verify the expression in mosue kidney tissue .I Never meet problems of background,it always shows strong signal for WB use 1:1000.
Date published: 2017-08-18
Rated 5 out of 5 by from clear signal bandI'm running western blots for FAS-L protein analysis,In my experience,this antibody can diluted at 1:500 to 1:1000. 1:200 is too much background.The loading amount and incubated conditions should be optimitized.
Date published: 2017-07-22
Rated 5 out of 5 by from Clean bandClean band observed in Western Blot analysis of FAS-L expression in HeLa whole cell lysate
Date published: 2017-05-24
Rated 5 out of 5 by from Worked goodThis antibody work well in the western blot with the dilution 1:800.
Date published: 2017-05-13
Rated 5 out of 5 by from Works greatWorks great for paraffin embedded human lung tissue sections,I suggest use dilution 1:300 for IF.
Date published: 2017-05-13
Rated 3 out of 5 by from Decent immunoperoxidase cytoplasmic stainingDecent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-05-13
Rated 3 out of 5 by from Published data for IP in human Ramos cellsPublished data for IP in human Ramos cells. -SCBT Publication Review
Date published: 2015-04-22
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FAS-L 抗体 (NOK-1) is rated 3.9 out of 5 by 14.
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