Date published: 2025-9-7

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Factor VII 抗体 (CaFVII-22): sc-101369

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说明书
  • Factor VII 抗体 CaFVII-22 是小鼠单克隆 IgG1 κ,Factor VII抗体, 在1篇文献中引用,规格为200 µg/ml
  • 免疫human物种的Factor VII
  • Factor VII 抗体 (CaFVII-22) 推荐用于WB, IF, IHC(P) 和 ELISA,检测human 来源的 the Gla-domain of Factor VII
  • 抗Factor VII抗体(CaFVII-22)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Factor VII (CaFVII-22): sc-101369免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 Factor VII 抗体 (CaFVII-22) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Factor VII 抗体 (CaFVII-22) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Factor VII抗体(CaFVII-22)是一种IgG1 κ小鼠单克隆Factor VII抗体(也称为Factor VII抗体),可通过WB、IF、IHC(P)和ELISA检测人源的Factor VII蛋白。Factor VII抗体(CaFVII-22)既可以是非偶联抗Factor VII抗体的形式,也可以是多种偶联形式的抗Factor VII抗体,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®偶联物。组织损伤后的止血涉及必需的血浆促凝血因子(凝血酶原和因子X、IX、V和VIII)的部署,这些因子参与导致不溶性纤维蛋白凝块形成和促进血小板聚集的血液凝固级联反应。Factor VII(血清凝血酶原转化加速剂、前转化素、F7、凝血因子7)是一种含有406个氨基酸的、维生素K依赖性的单链丝氨酸蛋白酶,在肝脏中合成并以无活性的前体形式循环。Factor IX A、Factor X A、Factor XII A或凝血酶介导的Factor VII在Arg 152-Ile 153处的蛋白水解切割产生Factor VII A,这是一种由催化重链通过二硫键与含有两个EGF样结构域的轻链连接而成的活性丝氨酸蛋白酶。导致Factor VII缺乏的F7位点突变通常是无症状的或表型未表征的,出血性素质发生在极低水平。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Factor VII 抗体 (CaFVII-22) 参考文献:

  1. 凝血酶通过蛋白酶活化受体-1 的蛋白水解激活作用,是结缔组织生长因子生成的强效诱导剂。  |  Chambers, RC., et al. 2000. J Biol Chem. 275: 35584-91. PMID: 10952976
  2. 因子 VII 缺乏症基因型与表型关系的分子分析。  |  Millar, DS., et al. 2000. Hum Genet. 107: 327-42. PMID: 11129332
  3. 因子 VII 缺乏症和 FVII 基因突变数据库。  |  McVey, JH., et al. 2001. Hum Mutat. 17: 3-17. PMID: 11139238
  4. 凝血级联:启动, 维持和调节。  |  Davie, EW., et al. 1991. Biochemistry. 30: 10363-70. PMID: 1931959
  5. 口服避孕药可增加血浆因子VII激活蛋白酶,并诱导体内因子VII激活。  |  Sidelmann, JJ., et al. 2011. Thromb Res. 128: e67-72. PMID: 21737124
  6. 血液凝固的基本机制  |  Davie, EW. and Fujikawa, K. 1975. Annu Rev Biochem. 44: 799-829. PMID: 237463
  7. 通过新的ELISA检测法评估先天性因子VII缺乏症中的因子VII抗原。  |  Tirindelli, MC., et al. 1987. Am J Hematol. 26: 313-21. PMID: 2961255
  8. 编码人类 VII 因子基因的核苷酸序列,VII 因子是一种参与血液凝固的依赖维生素 K 的蛋白质。  |  O'Hara, PJ., et al. 1987. Proc Natl Acad Sci U S A. 84: 5158-62. PMID: 3037537
  9. 人类 VII 因子编码 cDNA 的特征。  |  Hagen, FS., et al. 1986. Proc Natl Acad Sci U S A. 83: 2412-6. PMID: 3486420
  10. 作为凝血因子VII检测的底物血浆,免疫耗竭血浆和遗传性凝血因子VII缺乏血浆的特征和比较。  |  Brandt, JT., et al. 1986. Am J Clin Pathol. 85: 583-9. PMID: 3518398
  11. 妊娠期凝血因子VII。  |  Dalaker, K. and Prydz, H. 1984. Br J Haematol. 56: 233-41. PMID: 6607066
  12. 凝血酶原复合物浓缩物中的凝血因子VII和活化凝血因子VII含量。  |  Fiedler, H. 1998. Vox Sang. 75: 72-3. PMID: 9779562

订购信息

产品名称产品编号规格价格数量收藏夹

Factor VII 抗体 (CaFVII-22)

sc-101369
200 µg/ml
RMB2377.00

Factor VII (CaFVII-22): m-IgG Fc BP-HRP 套装

sc-527065
200 µg Ab; 10 µg BP
RMB2662.00

Factor VII (CaFVII-22): m-IgG1 BP-HRP 套装

sc-532438
200 µg Ab; 20 µg BP
RMB2662.00

Factor VII 抗体 (CaFVII-22) AC

sc-101369 AC
500 µg/ml, 25% agarose
RMB3129.00

Factor VII 抗体 (CaFVII-22) HRP

sc-101369 HRP
200 µg/ml
RMB2377.00

Factor VII 抗体 (CaFVII-22) FITC

sc-101369 FITC
200 µg/ml
RMB2482.00

Factor VII 抗体 (CaFVII-22) PE

sc-101369 PE
200 µg/ml
RMB2580.00

Factor VII 抗体 (CaFVII-22) Alexa Fluor® 488

sc-101369 AF488
200 µg/ml
RMB2685.00

Factor VII 抗体 (CaFVII-22) Alexa Fluor® 546

sc-101369 AF546
200 µg/ml
RMB2685.00

Factor VII 抗体 (CaFVII-22) Alexa Fluor® 594

sc-101369 AF594
200 µg/ml
RMB2685.00

Factor VII 抗体 (CaFVII-22) Alexa Fluor® 647

sc-101369 AF647
200 µg/ml
RMB2685.00

Factor VII 抗体 (CaFVII-22) Alexa Fluor® 680

sc-101369 AF680
200 µg/ml
RMB2685.00

Factor VII 抗体 (CaFVII-22) Alexa Fluor® 790

sc-101369 AF790
200 µg/ml
RMB2685.00

I have a construction that has a FVII gla domain at C terminal position and I would like to see if it has been gama carboxylated. Can this antibody be useful to detect the gama carboxylation of this domain? Thanks

Asked by: Luciano
Thank you for your question. The exact epitope of Factor VII Antibody (CaFVII-22): sc-101369 on its target protein has not been mapped yet, so there is no information that would indicate whether or not the antibody would recognize the C-terminus specifically or differentiate between carboxylated and non-carboxylated FVII. Please contact our Technical Service Department to arrange a free 10 µg sample of Factor VII (CaFVII-22): sc-101369 antibody to try it out in your experimental setup.
Answered by: Tech Support Europe
Date published: 2020-06-25

Do you have any data, whether the antibody might also recognize murine F7?

Asked by: mabeln
Thank you for your question. Factor VII Antibody (CaFVII-22): sc-101369 is only recommended for detection of the Gla-domain of Factor VII of human origin by WB, IF, IHC(P) and ELISA. This recommendation is based on our tests with this antibody in our labs using our standard protocols. However, there is a publication from 2018 in which this antibody has been used successfully to detect F7 in murine Schwannoma cells: PMID 29292076.
Answered by: Tech Support Europe
Date published: 2019-07-24

I am using Factor VII (CaFVII-22): sc-101369 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Nice for IP imagesWe used this antibody for Immunoperoxidase staining. We were able to take nice images of the stained cells
Date published: 2017-02-02
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Factor VII 抗体 (CaFVII-22) is rated 4.5 out of 5 by 2.
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