Date published: 2025-9-8
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F4/80 Antibody (C-7) - Immunohistochemistry - Image 287093
F4/80 抗体 (C-7): sc-377009. 福尔马林固定, 石蜡包埋的人支气管组织的免疫过氧化物酶染色显示呼吸道上皮细胞的胞浆染色.
F4/80 抗体 (C-7): sc-377009. 福尔马林固定, 石蜡包埋的人支气管组织的免疫过氧化物酶染色显示呼吸道上皮细胞的胞浆染色.

F4/80 抗体 (C-7): sc-377009

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说明书
  • F4/80 抗体 C-7 是小鼠单克隆 IgG1 κ，F4/80抗体, 在173篇文献中引用，规格为200 µg/ml
  • 针对氨基酸335-634的抗原，在mouse起源的F4/80的细胞外域内
  • F4/80 抗体 (C-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测 EMR1 of human origin, F4/80 of mouse origin and the corresponding rat homolog
  • 抗F4/80抗体（C-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取F4/80 (C-7): sc-377009的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 F4/80 抗体 (C-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与F4/80 抗体 (C-7) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 EMR and F4/80 抗体包括 EMR1 和 F4/80

F4/80 抗体 (C-7) 是一种 IgG1 κ 小鼠单克隆 F4/80 抗体（又称粘附 G 蛋白偶联受体 E1 抗体、Adgre1 抗体、类表皮生长因子模块受体 1 抗体或 Emr1 抗体），可检测人源的 EMR1 蛋白、小鼠源的 F4/80 蛋白和相应的大鼠同源物。F4/80 抗体 (C-7) 既有非结合型抗 F4/80 抗体，也有多种结合型抗 F4/80 抗体，包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 结合物。表皮生长因子（EGF）-TM7 家族是一组 B 类 G 蛋白偶联受体，包括 CD97、EMR1（含有粘蛋白类激素受体的类表皮生长因子分子 1，小鼠命名为 F4/80）、EMR2、EMR3、FIRE 和 ETL。这些家族成员的特征是具有多个 N 端 EGF 结构域的扩展胞外区，主要在免疫系统细胞中表达。EGF-TM7 蛋白家族由人类染色体 19p13 上的一个基因簇编码。F4/80 分子只在巨噬细胞表面表达，是成熟巨噬细胞组织的标志物，包括肝脏中的 Kupffer 细胞、脾脏红髓巨噬细胞、脑小胶质细胞、肠道固有层和皮肤中的朗格汉斯细胞。F4/80/EMR1 经过广泛的 N-连接糖基化和一些 O-连接糖基化。F4/80/EMR1 的功能尚不清楚，但推测它参与巨噬细胞粘附事件、细胞迁移或作为巨噬细胞的 G 蛋白偶联信号成分。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

F4/80 抗体 (C-7) 参考文献:

  1. 人类 EMR2 是染色体 19p13.1 上的一种新型 EGF-TM7 分子，与 CD97 关系密切。  |  Lin, HH., et al. 2000. Genomics. 67: 188-200. PMID: 10903844
  2. 人类 EGF-TM7 家族成员 EMR2 是一种表达于髓系细胞的异源二聚体受体。  |  Kwakkenbos, MJ., et al. 2002. J Leukoc Biol. 71: 854-62. PMID: 11994511
  3. 小鼠种系中的 F4/80 糖蛋白失活。  |  Schaller, E., et al. 2002. Mol Cell Biol. 22: 8035-43. PMID: 12391169
  4. 对小鼠 Emr1 启动子的功能分析发现了巨噬细胞中高水平基因表达所需的新型富嘌呤调控基序。  |  O'Reilly, D., et al. 2004. Genomics. 84: 1030-40. PMID: 15533720
  5. EMR1是激素受体家族中一个不寻常的成员，具有七个跨膜片段。  |  Baud, V., et al. 1995. Genomics. 26: 334-44. PMID: 7601460
  6. 巨噬细胞表面糖蛋白 F4/80 是一种高度糖基化的蛋白聚糖。  |  Haidl, ID. and Jefferies, WA. 1996. Eur J Immunol. 26: 1139-46. PMID: 8647179
  7. 出生后大鼠脑垂体小胶质细胞和巨噬细胞的发育  |  Mander, TH. and Morris, JF. 1996. Cell Tissue Res. 286: 347-55. PMID: 8929337
  8. Emr1 基因的染色体图谱。  |  McKnight, AJ., et al. 1997. Mamm Genome. 8: 946. PMID: 9383301
  9. EMR1和CD97在人类19号染色体上的物理映射以及Cd97在小鼠8号染色体上的分配表明了一个古老的基因组重复。  |  Carver, EA., et al. 1999. Mamm Genome. 10: 1039-40. PMID: 10501980

订购信息

产品名称产品编号规格价格数量收藏夹

F4/80 抗体 (C-7)

sc-377009
200 µg/ml
RMB2377.00

F4/80 (C-7): m-IgG Fc BP-HRP 套装

sc-529956
200 µg Ab; 10 µg BP
RMB2662.00

F4/80 (C-7): m-IgGκ BP-HRP 套装

sc-523150
200 µg Ab, 40 µg BP
RMB2662.00

F4/80 (C-7): m-IgG1 BP-HRP 套装

sc-543786
200 µg Ab; 20 µg BP
RMB2662.00

F4/80 抗体 (C-7) AC

sc-377009 AC
500 µg/ml, 25% agarose
RMB3129.00

F4/80 抗体 (C-7) HRP

sc-377009 HRP
200 µg/ml
RMB2377.00

F4/80 抗体 (C-7) FITC

sc-377009 FITC
200 µg/ml
RMB2482.00

F4/80 抗体 (C-7) PE

sc-377009 PE
200 µg/ml
RMB2580.00

F4/80 抗体 (C-7) Alexa Fluor® 488

sc-377009 AF488
200 µg/ml
RMB2685.00

F4/80 抗体 (C-7) Alexa Fluor® 546

sc-377009 AF546
200 µg/ml
RMB2685.00

F4/80 抗体 (C-7) Alexa Fluor® 594

sc-377009 AF594
200 µg/ml
RMB2685.00

F4/80 抗体 (C-7) Alexa Fluor® 647

sc-377009 AF647
200 µg/ml
RMB2685.00

F4/80 抗体 (C-7) Alexa Fluor® 680

sc-377009 AF680
200 µg/ml
RMB2685.00

F4/80 抗体 (C-7) Alexa Fluor® 790

sc-377009 AF790
200 µg/ml
RMB2685.00

这个抗体是用什么修复方式？

Asked by: yll245
感谢您的提问。如果您能直接拨打(+86) 021-60936350，或者发邮件到我们亚洲服务部的邮箱[email protected]我们就这个问题和其他相关问题进行更多的互动讨论，那将会很有帮助。 Thank you for your question. It would be helpful if you could email us directly at [email protected]. So we can have a more interactive discussion about this and other related issues.
Answered by: Zhanglong
Date published: 2024-03-28

hi. Can sc-377009 product use for hamster samples?

Asked by: Minji
Thank you for your question. This antibody has not been tested for hamster in house, however there are two publications citing hamster use for this antibody. You can find them on PubMed (PMID: 24342243 and PMID:25136608).
Answered by: Tech Service
Date published: 2019-09-23

Can this antibody be used in FCM to screen the macrophage of rat?

Asked by: Linlic
The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2021-11-24

Can this antibody be used in paraffin-embedded sections?

Asked by: Anonymous
Thank you for your question. Yes, F4/80 (C-7): sc-377009 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 15
Date published: 2025-06-26

What is a good positive control to use with the SIRT1 (B-7) sc-74465 antibody?

Asked by: Anonymous
We recommend K-562 nuclear extract: sc-2130, RAW 264.7 whole cell lysate: sc-2211, HL-60 whole cell lysate: sc-2209, M1 whole cell lysate: sc-364782, and WEHI-3 whole cell lysate: sc-3815. We have Western blot data showing specific detection of SIRT1 in these cell lines.
Answered by: Technical Support 3
Date published: 2017-01-09
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Rated 5 out of 5 by from Good! Thanks.This antibody shows good results in IHC for mouse samples.
Date published: 2021-10-15
Rated 5 out of 5 by from Great for IHCI used this antibody for staining of mouse liver samples. It worked well.
Date published: 2020-12-19
Rated 4 out of 5 by from Good in IHCUsed on human cancer cells, and have good signals when using it.
Date published: 2017-10-19
Rated 5 out of 5 by from perfect in IFI used this antibody before 4 days ago. The product shows perfect quality. The samples are breast cancer masses of BalB/C mice. Macrophages were detected clearly with F4/80 antibody. The dilution was 1:200.
Date published: 2017-09-14
Rated 3 out of 5 by from F4/80 is no bindWB 1:1000 5%BSA Block. primary antibody 4℃ overnight secondary antibody HRP, RT, 1hour.
Date published: 2017-09-02
Rated 4 out of 5 by from GreatMacrophages in rat lung tissue were detected with F4/80 antibody, and F4/80 antibody worked well on IF of paraffin sections. We started dillution was 1:200.
Date published: 2017-08-15
Rated 1 out of 5 by from Not workingI checked this Ab for mouse tissue staining, but got no signal.
Date published: 2017-08-14
Rated 5 out of 5 by from Wonderful Result!I used it for IF (frozen section) of mouse tumor at a dilution of 1:200 to identify macrophages in tumor tissue, and the staining result was wonderful!
Date published: 2017-08-13
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F4/80抗体 (C-7) is rated 4.4 out of 5 by 22.
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