Date published: 2025-9-7

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F4/80 抗体 (BM8): sc-52664

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说明书
  • F4/80 抗体 BM8 是大鼠单克隆 IgG2a, 在107篇文献中引用,提供 100 µg/ml
  • 针对mouse来源的骨髓来源培养巨噬细胞
  • 推荐用于 EMR1 of human origin, F4/80 of mouse origin and the corresponding rat homolog WB, IF, IHC(P) 和 FCM检测
  • 看 F4/80 (C-7): sc-377009,可查看有标记的 F4/80 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取F4/80 (BM8): sc-52664免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成F4/80 抗体 (BM8)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

F4/80 抗体 (BM8) 是一种 IgG2a 大鼠单克隆 F4/80 抗体,可通过 WB、IF、IHC(P) 和 FCM 检测人源的 EMR1 蛋白、鼠源的 F4/80 蛋白和相应的大鼠同源物。F4/80 抗体 (BM8) 以非结合型抗 F4/80 抗体形式提供。表皮生长因子(EGF)-TM7 家族是一组 B 类 G 蛋白偶联受体,包括 CD97、EMR1(含粘蛋白类激素受体的表皮生长因子样分子 1,小鼠命名为 F4/80)、EMR2、EMR3、FIRE 和 ETL。这些家族成员的特征是具有多个 N 端 EGF 结构域的扩展胞外区,主要在免疫系统细胞中表达。EGF-TM7 蛋白家族由人类染色体 19p13 上的一个基因簇编码。F4/80 分子只在巨噬细胞表面表达,是成熟巨噬细胞组织的标志物,包括肝脏中的 Kupffer 细胞、脾脏红髓巨噬细胞、脑小胶质细胞、肠道固有层和皮肤中的朗格汉斯细胞。F4/80/EMR1 经过广泛的 N-连接糖基化和一些 O-连接糖基化。F4/80/EMR1 的功能尚不清楚,但推测它参与巨噬细胞粘附事件、细胞迁移或作为巨噬细胞的 G 蛋白偶联信号成分。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

F4/80 抗体 (BM8)

sc-52664
100 µg/ml
RMB2377.00

Can this antibody be used in rat tissue?

Asked by: Josiane
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

I will use it for formalin-fixed mouse sections. Does citrate unmasking buffer work besides EDTA?

Asked by: Juba
Thank you for your question. Yes, we do recommend sodium citrate for antigen retrieval, although EDTA is also a good method. You can find more information in our recommended protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Tech Service
Date published: 2019-08-08

How many tests could i get out of 100ug with a 1:250 dillution

Asked by: jjk18
Thank you for your question. This antibody is provided at 100µg in 1ml. In dilutions, you multiply the dilution factors for each step. The dilution factor or the dilution is the initial volume divided by the final volume. (DF=Vi/Vf) For example, if you add a 1 mL sample to 9 mL of diluent to get 10 mL of solution, DF=Vi/Vf = 1mL / 10mL= 1/ 10. This is a 1:10 dilution.
Answered by: Tech Service
Date published: 2021-07-19

What is the total volume provided of sc-52664:F4/80 (BM8)?

Asked by: jenni
Each vial contains 100 µg of F4/80 (BM8) mouse monoclonal IgG2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support 2
Date published: 2017-01-09
  • y_2025, m_8, d_30, h_9CST
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Rated 2 out of 5 by from 非特異的染色が見られるマウスの胃組織の染色を行ったところ、マクロファージは染色されましたが、それ以外の細胞で非特異的な染色が見られました。
Date published: 2023-06-06
Rated 5 out of 5 by from SK030204V100Tested on murine macrophages by IF, quite good antibody
Date published: 2022-01-19
Rated 4 out of 5 by from good antibodyTested on murine macrophages by IF, quite good antibody
Date published: 2021-11-06
Rated 4 out of 5 by from Good!This antibody shows good results in IHC for mouse samples.
Date published: 2021-10-15
Rated 5 out of 5 by from IHC paraffin-embedded mouse liver sectionI have struggled F4/80 staining, but by using the AB (sc-56624), I got the best quality of staining.
Date published: 2018-11-09
Rated 4 out of 5 by from IHC(paraffin) liver tissueUse EDTA for antigen retrieval and get good results
Date published: 2018-01-15
Rated 5 out of 5 by from Quality1:500 dilution, mouse liver. Good quality IHC staining. (Trasino et al. J Mol Med 2016)
Date published: 2017-11-17
Rated 5 out of 5 by from clear bandsclear bands in Western Blot, also performs well in IF staining,specific staining.
Date published: 2017-05-13
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F4/80 抗体 (BM8) is rated 4.5 out of 5 by 15.
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