Date published: 2025-9-8
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Ezrin Antibody (4A5) - Western Blotting - Image 9931
Ezrin 抗体 (4A5): sc-32759. HeLa (A), A-431 (B), Jurkat (C) 和 F9 (D) 全细胞裂解液中 Ezrin 表达的 Western 印迹分析.
Ezrin 抗体 (4A5): sc-32759. HeLa (A), A-431 (B), Jurkat (C) 和 F9 (D) 全细胞裂解液中 Ezrin 表达的 Western 印迹分析.

Ezrin 抗体 (4A5): sc-32759

4.1(10)
寫評論提問

说明书
  • Ezrin抗体(4A5)是小鼠单克隆IgG1 κ, 在16篇文献中引用，规格为200 µg/ml
  • 针对Ezrin纯化的rabbit来源胃壁细胞
  • 抗-Ezrin 抗体 (4A5) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat, human 和rabbit 来源的 Ezrin
  • 抗Ezrin抗体（4A5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ezrin (4A5): sc-32759的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Ezrin 抗体 (4A5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Ezrin 抗体 (4A5) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Ezrin抗体（4A5）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学等方法检测小鼠、大鼠、人类和兔子的Ezrin样品。抗Ezrin抗体（4A5）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Ezrin在细胞结构和信号传导中发挥着关键作用，是细胞骨架和质膜之间的关键纽带。Ezrin主要位于质膜下方，负责促进肌动蛋白丝的组织和膜突起的形成，这对于细胞迁移、粘附和形状维持等过程至关重要。此外，Ezrin还是包括表皮生长因子受体在内的多种蛋白酪氨酸激酶的重要底物，并作为cAMP依赖性蛋白激酶（AKAP78）的锚定蛋白发挥作用。Ezrin、Moesin和Radixin具有独特的磷酸化模式，在不同细胞环境中发挥着独特的调节作用，因此Ezrin对于理解细胞信号传导和结构完整性至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Ezrin 抗体 (4A5)

sc-32759
200 µg/ml
RMB2377.00

Ezrin (4A5): m-IgG Fc BP-HRP 套装

sc-526687
200 µg Ab; 10 µg BP
RMB2662.00

Ezrin (4A5): m-IgG1 BP-HRP 套装

sc-532060
200 µg Ab; 20 µg BP
RMB2662.00

Ezrin 抗体 (4A5) AC

sc-32759 AC
500 µg/ml, 25% agarose
RMB3129.00

Ezrin 抗体 (4A5) HRP

sc-32759 HRP
200 µg/ml
RMB2377.00

Ezrin 抗体 (4A5) FITC

sc-32759 FITC
200 µg/ml
RMB2482.00

Ezrin 抗体 (4A5) PE

sc-32759 PE
200 µg/ml
RMB2580.00

Ezrin 抗体 (4A5) Alexa Fluor® 488

sc-32759 AF488
200 µg/ml
RMB2685.00

Ezrin 抗体 (4A5) Alexa Fluor® 546

sc-32759 AF546
200 µg/ml
RMB2685.00

Ezrin 抗体 (4A5) Alexa Fluor® 594

sc-32759 AF594
200 µg/ml
RMB2685.00

Ezrin 抗体 (4A5) Alexa Fluor® 647

sc-32759 AF647
200 µg/ml
RMB2685.00

Ezrin 抗体 (4A5) Alexa Fluor® 680

sc-32759 AF680
200 µg/ml
RMB2685.00

Ezrin 抗体 (4A5) Alexa Fluor® 790

sc-32759 AF790
200 µg/ml
RMB2685.00

I am using Ezrin (4A5): sc-32759 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Overall very good for western blotThis antibody is great for western blot analysis and shows a specific band in mouse cerebellar extracts. When staining mixed primary cerebellar cultures there is no specific staining. The staining appears similar to background seen in the system and therefore appears not to be suitable for primary cerebellar staining. There was no obvious detection in cerebellar Purkinje neurons.
Date published: 2018-01-30
Rated 5 out of 5 by from Nice western blot results in JurkatWe used this antibody for expression detection of Ezrin in western blot. We got a nice band around the correct molecular weight in Jurkat cells.
Date published: 2017-02-06
Rated 4 out of 5 by from Adapted for westernblot on human tissue (one band)Adapted for westernblot on human tissue (one band)
Date published: 2015-10-28
Rated 4 out of 5 by from Published data for WBPublished data for WB, IP and IF in rat uterus. -SCBT Publication Review
Date published: 2015-07-09
Rated 4 out of 5 by from Published data for WB and IF in human CD4+Published data for WB and IF in human CD4+ CXCR4+ CEM cells, Jurkat cell, peripheral blood lymphocytes (PBLs), HeLa P4 cells, and HeLa 243 cells. -SCBT Publication Review
Date published: 2015-07-01
Rated 4 out of 5 by from Published data for WB in human BLM cellsPublished data for WB in human BLM cells. -SCBT Publication Review
Date published: 2015-05-09
Rated 4 out of 5 by from Published data for WB in human MePublished data for WB in human Me-180 HEC-1-B and VK2/E6E6 cells. -SCBT Publication Review
Date published: 2015-05-08
Rated 4 out of 5 by from Good positive signal observed in WB in JurkatGood positive signal observed in WB in Jurkat, A-431, HeLa and F9 whole cell lysates. -SCBT QC
Date published: 2015-03-10
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Ezrin 抗体 (4A5) is rated 4.1 out of 5 by 10.
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