Date published: 2025-9-8

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Ets-2 抗体 (E-5): sc-365666

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说明书
  • Ets-2抗体(E-5)是小鼠单克隆IgG1 κ, 在13篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Ets-2的C-terminus的氨基酸441-469之间
  • 抗-Ets-2 抗体 (E-5) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Ets-2; 也和以下物种反应,包括: 和 canine and bovine
  • 可用于ChIP的TransCruz试剂(sc-365666 X, 200 µg/0.1 ml)
  • 抗Ets-2抗体(E-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ets-2 (E-5): sc-365666免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是Ets-2 Antibody (E-5) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Ets-2 Antibody (E-5) 打包提供(请参阅下面的订购信息)。

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関連項目

Ets-2抗体(E-5)是一种IgG1κ小鼠单克隆Ets-2抗体(也称为ETS2抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的Ets-2。Ets-2抗体(E-5)可以以非偶联的抗Ets-2抗体形式以及多种偶联形式的抗Ets-2抗体形式提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Ets-1是基于与分离自E26成红细胞增多症病毒的v-Ets癌基因的同源性而鉴定的基因家族的原型成员。该基因家族目前包括Ets-1、Ets-2、Erg-1-3、Elk-1、Elf-1、Elf-5、NERF、PU.1、PEA3、ERM、FEV、ER8l、Fli-1、TEL、Spi-B、ESE-1、ESE-3A、Net、ABT1和ERF。Ets基因家族成员表现出不同的组织表达模式,并共享一个高度保守的羧基末端结构域,该结构域包含与SV40大T抗原核定位信号序列相关的序列。这个保守的结构域对于Ets-1与DNA的结合是必不可少的,并且可能是Ets基因家族所有成员DNA结合活性的原因。其中一些蛋白质已被证明可以识别DNA中共享中央5'-GGAA-3'元件的相似基序。有证据表明,Ets-1的DNA结合活性是在磷酸化水平上调节的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ets-2 抗体 (E-5) 参考文献:

  1. Ets-2和哺乳动物SWI/SNF的组成部分形成抑制复合物,对BRCA1启动子产生负调控作用。  |  Baker, KM., et al. 2003. J Biol Chem. 278: 17876-84. PMID: 12637547
  2. PEA3的分子克隆和特征描述,PEA3是Ets癌基因家族的一个新成员,在小鼠胚胎细胞中有不同表达。  |  Xin, JH., et al. 1992. Genes Dev. 6: 481-96. PMID: 1547944
  3. ets-2促进唐氏综合征神经元线粒体死亡途径的激活。  |  Helguera, P., et al. 2005. J Neurosci. 25: 2295-303. PMID: 15745955
  4. 推测的肿瘤抑制基因 HCCS1 的转录需要 ETS-2 与其转录起始位点附近的共识结合。  |  Zhu, JD., et al. 2006. Cell Res. 16: 780-96. PMID: 16953216
  5. Ets-2和C/EBP-beta是滋养层中绵羊滋养层Kunitz结构域蛋白-1基因表达的重要介导因子。  |  Chakrabarty, A. and Roberts, MR. 2007. BMC Mol Biol. 8: 14. PMID: 17326832
  6. 果蝇 74EF 早期浮肿包含 E74,这是一个复杂的蜕皮激素诱导基因,编码两种与蜕皮激素相关的蛋白质。  |  Burtis, KC., et al. 1990. Cell. 61: 85-99. PMID: 2107982
  7. 转录因子ets-2在肺纤维化的发病机理中起着重要作用。  |  Baran, CP., et al. 2011. Am J Respir Cell Mol Biol. 45: 999-1006. PMID: 21562315
  8. 麋鹿,X 和 14 号染色体上与组织特异性等位基因相关的基因,靠近易位断点。  |  Rao, VN., et al. 1989. Science. 244: 66-70. PMID: 2539641
  9. c-ets 癌基因编码的 54,000 细胞质蛋白在胸腺和法氏囊淋巴细胞中的鉴定和优先表达。  |  Ghysdael, J., et al. 1986. Proc Natl Acad Sci U S A. 83: 1714-8. PMID: 3006066
  10. 21 号染色体上的人类 ets 相关基因 erg:替代剪接, 多腺苷酸化和翻译。  |  Rao, VN., et al. 1987. Science. 237: 635-9. PMID: 3299708
  11. Ets1 转录因子在小鼠胚胎发育过程中广泛表达,与参与器官形成等形态发生过程的中胚层细胞有关。  |  Kola, I., et al. 1993. Proc Natl Acad Sci U S A. 90: 7588-92. PMID: 7689222
  12. PU.1 磷酸化对与 NF-EM5 的相互作用和转录激活的影响。  |  Pongubala, JM., et al. 1993. Science. 259: 1622-5. PMID: 8456286

订购信息

产品名称产品编号规格价格数量收藏夹

Ets-2 抗体 (E-5)

sc-365666
200 µg/ml
RMB2377.00

Ets-2 (E-5): m-IgG Fc BP-HRP 套装

sc-529430
200 µg Ab; 10 µg BP
RMB2662.00

Ets-2 (E-5): m-IgGκ BP-HRP 套装

sc-522318
200 µg Ab, 40 µg BP
RMB2662.00

Ets-2 (E-5): m-IgG1 BP-HRP 套装

sc-543514
200 µg Ab; 20 µg BP
RMB2662.00

Ets-2 抗体 (E-5) X

sc-365666 X
200 µg/0.1 ml
RMB2377.00

Ets-2 抗体 (E-5) AC

sc-365666 AC
500 µg/ml, 25% agarose
RMB3129.00

Ets-2 抗体 (E-5) HRP

sc-365666 HRP
200 µg/ml
RMB2377.00

Ets-2 抗体 (E-5) FITC

sc-365666 FITC
200 µg/ml
RMB2482.00

Ets-2 抗体 (E-5) PE

sc-365666 PE
200 µg/ml
RMB2580.00

Ets-2 抗体 (E-5) Alexa Fluor® 488

sc-365666 AF488
200 µg/ml
RMB2685.00

Ets-2 抗体 (E-5) Alexa Fluor® 546

sc-365666 AF546
200 µg/ml
RMB2685.00

Ets-2 抗体 (E-5) Alexa Fluor® 594

sc-365666 AF594
200 µg/ml
RMB2685.00

Ets-2 抗体 (E-5) Alexa Fluor® 647

sc-365666 AF647
200 µg/ml
RMB2685.00

Ets-2 抗体 (E-5) Alexa Fluor® 680

sc-365666 AF680
200 µg/ml
RMB2685.00

Ets-2 抗体 (E-5) Alexa Fluor® 790

sc-365666 AF790
200 µg/ml
RMB2685.00

Ets-2 (E-5) 中和勝肽

sc-365666 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-365666 P appropriate for?

Asked by: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from does not work for EMSADoes not super shift for EMSA, please bring back C-20.
Date published: 2021-05-27
Rated 2 out of 5 by from goodnot bad, it work well in some tissue. However the band is not very clear
Date published: 2018-10-10
Rated 4 out of 5 by from guter Antikörper für Western BlotVerwendung 1:500 für Western Blot mit Zelllysaten hoher Hintergrund, trotzdem spezifische Banden, lange Belichtungszeit benötigt
Date published: 2017-11-22
Rated 5 out of 5 by from Good antibodyI tried this antibody for WB with 1:666 dilution and second antibody 1:2000. The band signal was medium to strong with relative low background and size was correct. It is a unquestionable good antibody.
Date published: 2017-08-18
Rated 5 out of 5 by from Works greatExcellent antibody! One single (and strong) band at the right molecular weight.
Date published: 2017-07-21
Rated 5 out of 5 by from clean bandThis monoclonal antibody gives a nice strong robust and clean band for WB analysis in NIH/3T3 whole cell lysate.
Date published: 2017-04-19
Rated 5 out of 5 by from Good nuclear signalGood nuclear signal in immunostaining of embryonic cardiomyocytes (mouse). Not tested in other applications.
Date published: 2017-04-03
Rated 1 out of 5 by from Many nonspecific bandsWe use it to detect HK2 cell , we usually use diluted 1:500 for WB can't get the desired results
Date published: 2017-03-25
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Ets-2 抗体 (E-5) is rated 4.0 out of 5 by 12.
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