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ETO Antibody (3H11) - Western Blotting - Image 366688
ETO 抗体 (3H11): sc-134335. AML-193 (A), CCRF-CEM (B), HL-60 (C), MOLT-4 (D) 和 Hep G2 (E) 全细胞裂解液中 ETO 表达的 Western 印迹分析.
ETO Antibody (3H11) - Western Blotting - Image 366688
ETO 抗体 (3H11): sc-134335. AML-193 (A), CCRF-CEM (B), HL-60 (C), MOLT-4 (D) 和 Hep G2 (E) 全细胞裂解液中 ETO 表达的 Western 印迹分析.
ETO 抗体 (3H11): sc-134335. AML-193 (A), CCRF-CEM (B), HL-60 (C), MOLT-4 (D) 和 Hep G2 (E) 全细胞裂解液中 ETO 表达的 Western 印迹分析.

ETO 抗体 (3H11): sc-134335

4.3(3)
寫評論提問

说明书
  • ETO 抗体 3H11 是小鼠单克隆 IgG2a κ，ETO抗体, 在3篇文献中引用，规格为100 µg/ml
  • 针对部分重组蛋白，其氨基酸序列在ETO的human起源的416-526范围内
  • 推荐用于 mouse, rat 和 human 来源的ETO WB, IP 和 ELISA检测
  • 关于如何获取ETO (3H11): sc-134335的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是ETO Antibody (3H11) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与ETO Antibody (3H11) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加ETO抗体，包括ETO-2

ETO抗体（3H11）是一种IgG2a κ小鼠单克隆ETO抗体（也称为ETO抗体），可通过WB、IP和ELISA检测小鼠、大鼠和人源的ETO蛋白。ETO抗体（3H11）可作为非偶联抗ETO抗体使用。ETO和ETO-2，可分别被命名为MTG8和MTG16，是ETO转录因子家族的成员。这些转录因子的特征在于一个锌指结构域和四个保守结构域，其中结构域II是家族成员之间二聚化所必需的。ETO和ETO-2可能起介导DNA结合蛋白和转录调节因子（如N-CoR）之间的相互作用的功能。ETO的t（8；21）易位经常产生AML-1/ETO癌蛋白，其由AML-1的前177个氨基酸和ETO中除前30个氨基酸以外的所有氨基酸组成。在12-15％的急性髓性M2亚型白血病中观察到AML-1/ETO的表达。AML-1/ETO融合蛋白与多聚体N-CoR/mSin3/HDAC1复合物相关，通过改变染色质重塑来阻断分化并诱导转录抑制。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ETO 抗体 (3H11) 参考文献:

  1. 核蛋白 ETO 家族的新成员 ETO-2。  |  Davis, JN., et al. 1999. Oncogene. 18: 1375-83. PMID: 10022820
  2. 组蛋白去乙酰化酶抑制剂可缓解 ETO 介导的抑制，并诱导 AML1-ETO 白血病细胞分化。  |  Wang, J., et al. 1999. Cancer Res. 59: 2766-9. PMID: 10383127
  3. 急性髓性白血病 t(8;21) 融合转录本的 ETO 部分编码一种高度进化保守的假定转录因子。  |  Erickson, PF., et al. 1994. Cancer Res. 54: 1782-6. PMID: 8137293
  4. ETO和AML1磷蛋白在CD34+造血祖细胞中表达：对t(8;21)白血病发生和监测残留疾病的意义。  |  Erickson, PF., et al. 1996. Blood. 88: 1813-23. PMID: 8781439
  5. t(8;21) 融合产物 AML-1-ETO 与 C/EBP-alpha 结合，抑制 C/EBP-alpha 依赖性转录，阻碍粒细胞分化。  |  Westendorf, JJ., et al. 1998. Mol Cell Biol. 18: 322-33. PMID: 9418879
  6. 人类 MTG8/ETO 基因的结构和表达。  |  Wolford, JK. and Prochazka, M. 1998. Gene. 212: 103-9. PMID: 9661669
  7. ETO是t(8;21)急性髓性白血病的融合伙伴，它通过与人类N-CoR/mSin3/HDAC1复合物相互作用抑制转录。  |  Wang, J., et al. 1998. Proc Natl Acad Sci U S A. 95: 10860-5. PMID: 9724795

订购信息

产品名称产品编号规格价格数量收藏夹

ETO 抗体 (3H11)

sc-134335
100 µg/ml
RMB2504.00

ETO (3H11): m-IgG Fc BP-HRP 套装

sc-537367
100 µg Ab; 10 µg BP
RMB2662.00

ETO (3H11): m-IgGκ BP-HRP 套装

sc-534614
100 µg Ab; 40 µg BP
RMB2662.00

ETO (3H11): m-IgG2a BP-HRP 套装

sc-547995
100 µg Ab; 10 µg BP
RMB2662.00

Is it possible to use ETO(3H11):sc-134335 to eTAFH domain-only (NHR1 domain)? Can you tell me "which domain" does the antibody recognize?

Asked by: daseul
Thank you for your question. This antibody was raised against a partial recombinant protein mapping within amino acids 416-526 of ETO of human origin. The exact epitope has not been mapped so I can not provide further information.
Answered by: Tech Service
Date published: 2019-10-02

For Western Blot, is it recommended to use denatured or non-denatured conditions with ETO (3H11): sc-134335 antibody?

Asked by: AbPolly
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by from Good for Western BlotAntibody detects AML1-ETO in FLAG-tag knock-in AML1-ETO (FLAG-AE) Kasumi-1 cell lysate by Western blot in published research -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from 3H11 detects both Mouse and Human protein.The sample antibody (ETO) worked really well. Even though raised against a human protein, the 3H11 clone identifies mouse proteins successfully.
Date published: 2017-02-07
Rated 3 out of 5 by from Positive results in western blot analysisPositive results in western blot analysis of ETO expression in human ETO transfected and non-transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-04-05
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ETO 抗体 (3H11) is rated 4.3 out of 5 by 3.
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