Date published: 2025-9-6
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ERK 1 Antibody (G-8) - Western Blotting - Image 349303
ERK 1 抗体 (G-8): sc-271269. MCF7 (A), THP-1 (B), DU 145 (C), KNRK (D), 3611-RF (E) 和 HEK293 (F) 全细胞裂解液中 ERK 1 表达的 Western 印迹分析.
ERK 1 抗体 (G-8): sc-271269. MCF7 (A), THP-1 (B), DU 145 (C), KNRK (D), 3611-RF (E) 和 HEK293 (F) 全细胞裂解液中 ERK 1 表达的 Western 印迹分析.

ERK 1 抗体 (G-8): sc-271269

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寫評論提問

说明书
  • ERK 1 抗体 G-8 是小鼠单克隆 IgG2b κ，ERK 1抗体, 在60篇文献中引用，规格为200 µg/ml
  • 特定于ERK 1的291-335亚域XI内氨基酸之间的表位图，rat起源
  • ERK 1 抗体 (G-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 ERK 1 p44; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 抗ERK 1抗体（G-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ERK 1 (G-8): sc-271269的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP是ERK 1 Antibody (G-8) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与ERK 1 Antibody (G-8) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外ERK抗体，包括ERK 1, ERK 2, ERK 3和ERK 5

ERK 1抗体（G-8）是一种IgG2b κ小鼠单克隆ERK 1抗体（也称为MAPK3抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的ERK 1蛋白。ERK 1抗体（G-8）既有非偶联抗ERK 1抗体形式，也有多种偶联形式的抗ERK 1抗体，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。有丝分裂原激活的蛋白激酶（MAPK）信号通路涉及两个密切相关的MAP激酶，即细胞外信号相关激酶1（ERK 1，p44）和2（ERK 2，p42）。生长因子、类固醇激素、G蛋白偶联受体配体和神经递质可以启动MAPK信号通路。ERK 1和ERK 2的激活需要上游激酶如MAP激酶激酶（MEK）、MEK激酶和Raf-1的磷酸化。ERK 1和ERK 2的磷酸化可以发生在包含苏氨酸-谷氨酸-酪氨酸基序的一致基序内映射的特定酪氨酸和苏氨酸位点。ERK的激活导致与其他ERKs的二聚化，并随后定位到细胞核。活性ERK二聚体磷酸化核蛋白上的丝氨酸和苏氨酸残基，并影响一系列反应，包括增殖、分化、转录调控和发育。人类ERK 1基因映射到染色体16p11.2，并编码一个由379个氨基酸组成的蛋白质，与ERK 2有83%的序列同一性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

ERK 1 抗体 (G-8)

sc-271269
200 µg/ml
RMB2377.00

ERK 1 (G-8): m-IgG2b BP-HRP 套装

sc-548698
200 µg Ab; 10 µg BP
RMB2662.00

ERK 1 抗体 (G-8) AC

sc-271269 AC
500 µg/ml, 25% agarose
RMB3129.00

ERK 1 抗体 (G-8) HRP

sc-271269 HRP
200 µg/ml
RMB2377.00

ERK 1 抗体 (G-8) FITC

sc-271269 FITC
200 µg/ml
RMB2482.00

ERK 1 抗体 (G-8) PE

sc-271269 PE
200 µg/ml
RMB2580.00

ERK 1 抗体 (G-8) Alexa Fluor® 488

sc-271269 AF488
200 µg/ml
RMB2685.00

ERK 1 抗体 (G-8) Alexa Fluor® 546

sc-271269 AF546
200 µg/ml
RMB2685.00

ERK 1 抗体 (G-8) Alexa Fluor® 594

sc-271269 AF594
200 µg/ml
RMB2685.00

ERK 1 抗体 (G-8) Alexa Fluor® 647

sc-271269 AF647
200 µg/ml
RMB2685.00

ERK 1 抗体 (G-8) Alexa Fluor® 680

sc-271269 AF680
200 µg/ml
RMB2685.00

ERK 1 抗体 (G-8) Alexa Fluor® 790

sc-271269 AF790
200 µg/ml
RMB2685.00

ERK 1 (G-8) 中和勝肽

sc-271269 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-271269 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Ottimo funzionamentoSono molto soddisfatto di questo anticorpo. L'ho utilizzato in WB su membrane di nitrocellulosa saturate con latte non grasso al 5% in TBS-T, e l'incubazione con l'anticorpo è stata effettuata a +4 °C o/n in TBS-T con latte al 2.5% (diluizione 1:1000).
Date published: 2025-04-09
Rated 1 out of 5 by from performed poorly for WB in our handsIt detected specific signals for both Erk1 and 2. But the sensitivity was very poor, with high background. Also, weak non-specific signals at ~125 and ~30 kDa.
Date published: 2019-05-29
Rated 4 out of 5 by from Works well for WB!Worked well for Western application against human cancer cell lysate samples.
Date published: 2018-03-23
Rated 4 out of 5 by from Pretty good for western blotUsed this antibody 1:1000 on a western blot using 100 ug of protein from tissue. Band was pretty clear and trustworthy.
Date published: 2018-02-21
Rated 5 out of 5 by from Working wellGood results used ERK1（G-8） Antibody in WB and IP.
Date published: 2018-01-21
Rated 5 out of 5 by from it is wonderfulI tested for SD rat vascular tissue samples,just one time,I got my results,I am so happy.thank you
Date published: 2017-11-15
Rated 3 out of 5 by from Good to recognize both ERK1 and ERK2...not specifiGood to recognize both ERK1 and ERK2...not specific for ERK2
Date published: 2017-10-13
Rated 4 out of 5 by from 总体还不错1:100稀释,湿转转膜1小时，蛋白上样量10微克，DAB发色，条带亮度较好，如果能提高效价将更理想。
Date published: 2017-10-13
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ERK 1 抗体 (G-8) is rated 4.6 out of 5 by 27.
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