Date published: 2025-9-6
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ERK 1 Antibody (E-6) - Western Blotting - Image 379450
ERK 1 抗体 (E-6): sc-271270. NIH/3T3 (A), K-562 (B), HL-60 (C), U-2 OS (D), ALL-SIL (E) 和 M1 (F) 全细胞裂解液中 ERK 1 表达的 Western 印迹分析.
ERK 1 抗体 (E-6): sc-271270. NIH/3T3 (A), K-562 (B), HL-60 (C), U-2 OS (D), ALL-SIL (E) 和 M1 (F) 全细胞裂解液中 ERK 1 表达的 Western 印迹分析.

ERK 1 抗体 (E-6): sc-271270

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说明书
  • ERK 1抗体(E-6)是小鼠单克隆IgG1 κ, 在32篇文献中引用，规格为200 µg/ml
  • 特定于ERK 1的291-335亚域XI内氨基酸之间的表位图，rat起源
  • 抗-ERK 1 抗体 (E-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 ERK 1 p44; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 抗ERK 1抗体（E-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ERK 1 (E-6): sc-271270的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是ERK 1 Antibody (E-6) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与ERK 1 Antibody (E-6) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外ERK抗体，包括ERK 1, ERK 2, ERK 3和ERK 5

ERK 1抗体（E-6）是一种IgG1 κ小鼠单克隆ERK 1抗体（也称为MAPK3抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人源的ERK 1 p44。ERK 1抗体（E-6）可作为非偶联抗ERK 1抗体形式，也可作为多种偶联形式的抗ERK 1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。丝裂原活化蛋白激酶（MAPK）信号通路涉及两个密切相关的MAP激酶，称为细胞外信号相关激酶1（ERK 1，p44）和2（ERK 2，p42）。生长因子、类固醇激素、G蛋白偶联受体配体和神经递质可启动MAPK信号通路。ERK 1和ERK 2的激活需要上游激酶如MAP激酶激酶（MEK）、MEK激酶和Raf-1的磷酸化。ERK 1和ERK 2的磷酸化可以发生在特定的酪氨酸和苏氨酸位点，这些位点在包含苏氨酸-谷氨酸-酪氨酸基序的一致基序内。ERK的激活导致与其他ERKs的二聚化并随后定位到细胞核。活性ERK二聚体磷酸化核蛋白上的丝氨酸和苏氨酸残基，并影响一系列反应，包括增殖、分化、转录调控和发育。人类ERK 1基因映射到染色体16p11.2，并编码一个包含379个氨基酸的蛋白质，与ERK 2的序列同一性为83%。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ERK 1 抗体 (E-6) 参考文献:

  1. 通过 p42/p44 MAP 激酶级联传递血管生成信号。  |  Pagès, G., et al. 2000. Ann N Y Acad Sci. 902: 187-200. PMID: 10865838
  2. G 蛋白偶联受体对丝裂原活化蛋白激酶信号网络的调控。  |  Gutkind, JS. 2000. Sci STKE. 2000: re1. PMID: 11752597
  3. MAP 激酶（ERKs）信号的保真度和时空控制。  |  Pouysségur, J., et al. 2002. Biochem Pharmacol. 64: 755-63. PMID: 12213567
  4. ERK1和ERK2是两种微管相关蛋白2激酶，它们在原位介导酪氨酸羟化酶丝氨酸-31的磷酸化。  |  Haycock, JW., et al. 1992. Proc Natl Acad Sci U S A. 89: 2365-9. PMID: 1347949
  5. MEK 的主要结构，MEK 是一种蛋白激酶，能使 ERK 基因产物磷酸化。  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
  6. 靶向细胞外信号调节激酶（ERK）信号对炎症性骨溶解具有治疗意义。  |  Seo, SW., et al. 2010. Bone. 46: 695-702. PMID: 19895919
  7. ERK：丝氨酸/苏氨酸蛋白激酶家族，在胰岛素和 NGF 的作用下被激活并酪氨酸磷酸化。  |  Boulton, TG., et al. 1991. Cell. 65: 663-75. PMID: 2032290
  8. ERK磷酸化作用和核内积累：单细胞成像的见解。  |  Caunt, CJ. and McArdle, CA. 2012. Biochem Soc Trans. 40: 224-9. PMID: 22260695
  9. 通过抑制细胞外信号调节激酶（ERK）信号通路，可减少猪急性腹泻综合征冠状病毒的复制。  |  Zhang, J., et al. 2022. Virology. 565: 96-105. PMID: 34768113
  10. 人类丝裂原活化蛋白激酶 p44erk1 的分子克隆, 表达和表征。  |  Charest, DL., et al. 1993. Mol Cell Biol. 13: 4679-90. PMID: 7687743
  11. 前列腺癌中过度表达的促分裂原活化蛋白激酶磷酸酶1与细胞凋亡呈负相关。  |  Magi-Galluzzi, C., et al. 1997. Lab Invest. 76: 37-51. PMID: 9010448
  12. 磷酸化 MAP 激酶 ERK2 可促进其同源二聚化和核转运。  |  Khokhlatchev, AV., et al. 1998. Cell. 93: 605-15. PMID: 9604935

订购信息

产品名称产品编号规格价格数量收藏夹

ERK 1 抗体 (E-6)

sc-271270
200 µg/ml
RMB2377.00

ERK 1 (E-6): m-IgG Fc BP-HRP 套装

sc-529153
200 µg Ab; 10 µg BP
RMB2662.00

ERK 1 (E-6): m-IgGκ BP-HRP 套装

sc-521897
200 µg Ab, 40 µg BP
RMB2662.00

ERK 1 (E-6): m-IgG1 BP-HRP 套装

sc-543357
200 µg Ab; 20 µg BP
RMB2662.00

ERK 1 抗体 (E-6) AC

sc-271270 AC
500 µg/ml, 25% agarose
RMB3129.00

ERK 1 抗体 (E-6) HRP

sc-271270 HRP
200 µg/ml
RMB2377.00

ERK 1 抗体 (E-6) FITC

sc-271270 FITC
200 µg/ml
RMB2482.00

ERK 1 抗体 (E-6) PE

sc-271270 PE
200 µg/ml
RMB2580.00

ERK 1 抗体 (E-6) Alexa Fluor® 488

sc-271270 AF488
200 µg/ml
RMB2685.00

ERK 1 抗体 (E-6) Alexa Fluor® 546

sc-271270 AF546
200 µg/ml
RMB2685.00

ERK 1 抗体 (E-6) Alexa Fluor® 594

sc-271270 AF594
200 µg/ml
RMB2685.00

ERK 1 抗体 (E-6) Alexa Fluor® 647

sc-271270 AF647
200 µg/ml
RMB2685.00

ERK 1 抗体 (E-6) Alexa Fluor® 680

sc-271270 AF680
200 µg/ml
RMB2685.00

ERK 1 抗体 (E-6) Alexa Fluor® 790

sc-271270 AF790
200 µg/ml
RMB2685.00

ERK 1 (E-6) 中和勝肽

sc-271270 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-271270 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good in WBThe quality of this antibody is good in western blotting. I get clear bands by using 1:1000.
Date published: 2021-07-21
Rated 5 out of 5 by from ExcellentI had a great signal and very specific. No unspecific binding was observed
Date published: 2021-03-22
Rated 4 out of 5 by from Worked well.Worked well for Western application against mouse samples.
Date published: 2018-03-23
Rated 5 out of 5 by from ERK 1 Western BlotPrimary cerebellar neurons conjugated with ERK 1 (1:1000). Very clear signal with little background.
Date published: 2018-03-09
Rated 2 out of 5 by from Okay for western blotUsed this antibody 1:1000 on a western blot with 100 ug of protein from tissue. Band was relatively obvious, but very weak. Compared this to identical western blot run side by side probed with Erk1 sc-271269 which was much better.
Date published: 2018-02-21
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of ERK 1 expression in HeLa and MCF7 whole cell lysates.
Date published: 2017-02-01
Rated 4 out of 5 by from This has worked well in WBThis has worked well in WB.
Date published: 2015-11-17
Rated 5 out of 5 by from IF: Very good nuclear and cytoplasmic stainingIF: Very good nuclear and cytoplasmic staining in HepG2 cells. -SCBT QC
Date published: 2015-03-27
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ERK 1 抗体 (E-6) is rated 4.4 out of 5 by 9.
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