Date published: 2025-9-6
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ERK 1/2 Antibody (MK1) - Western Blotting - Image 398962
ERK 1/2 抗体 (MK1): sc-135900. RAW 264. 7 (A) 和 KNRK (B) 全细胞裂解液及大鼠脑组织提取物 (C) 中 ERK 1/2 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
ERK 1/2 抗体 (MK1): sc-135900. RAW 264. 7 (A) 和 KNRK (B) 全细胞裂解液及大鼠脑组织提取物 (C) 中 ERK 1/2 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

ERK 1/2 抗体 (MK1): sc-135900

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说明书
  • ERK 1/2抗体(MK1)是小鼠单克隆IgG1 κ, 在325篇文献中引用，规格为200 µg/ml
  • 免疫rat物种的ERK 1的氨基酸325-345
  • 推荐用于 mouse, rat, human 和 canine 来源的ERK 1 and ERK 2 WB, IP 和 IF检测
  • 看 ERK 2 (D-2): sc-1647，可查看有标记的 ERK 1/2 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取ERK 1/2 (MK1): sc-135900的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是ERK 1/2 Antibody (MK1) for WB applications. 的首选辅助检测试剂，这些试剂现与ERK 1/2 Antibody (MK1) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

附加ERK抗体，包括ERK 1, ERK 2, ERK 3和ERK 5

ERK 1/2抗体（MK1）是一种IgG1 κ小鼠单克隆ERK 1/2抗体（也称为p44/42 MAPK抗体），可通过WB、IP和IF检测小鼠、大鼠和人源的ERK 1和ERK 2。ERK 1/2抗体（MK1）可作为非偶联抗ERK 1/2抗体形式。丝裂原活化蛋白激酶（MAPK）信号通路涉及两个密切相关的MAP激酶，称为细胞外信号相关激酶1（ERK 1，p44）和2（ERK 2，p42）。生长因子、类固醇激素、G蛋白偶联受体配体和神经递质可启动MAPK信号通路。ERK 1和ERK 2的激活需要上游激酶如MAP激酶激酶（MEK）、MEK激酶和Raf-1的磷酸化。ERK 1和ERK 2的磷酸化可以发生在特定的酪氨酸和苏氨酸位点，这些位点在包含苏氨酸-谷氨酸-酪氨酸基序的一致基序内。ERK的激活导致与其他ERKs的二聚化并随后定位到细胞核。活性ERK二聚体磷酸化核蛋白上的丝氨酸和苏氨酸残基，并影响一系列反应，包括增殖、分化、转录调控和发育。人类ERK 1基因映射到染色体16p11.2，并编码一个包含379个氨基酸的蛋白质，与ERK 2的序列同一性为83%。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ERK 1/2 抗体 (MK1) 参考文献:

  1. 在易患柯萨奇病毒诱导的心肌炎的小鼠中增强ERK-1/2的激活作用。  |  Opavsky, MA., et al. 2002. J Clin Invest. 109: 1561-9. PMID: 12070303
  2. 紫草素在3T3-L1细胞脂肪细胞分化早期抑制ERK 1/2的磷酸化作用。  |  Gwon, SY., et al. 2013. BMC Complement Altern Med. 13: 207. PMID: 23919458
  3. ERK 1/2激活介导BpV(pic)在局灶性脑缺血再灌注损伤中的神经保护作用  |  Liu, R., et al. 2018. Neurochem Res. 43: 1424-1438. PMID: 29882124
  4. 抑制 ERK 1/2激酶可防止肌腱基质分解。  |  Blache, U., et al. 2021. Sci Rep. 11: 6838. PMID: 33767224
  5. 癌症中mTOR/ERK-1/2相关蛋白质的种族差异表达。  |  Mishra, S., et al. 2021. Front Cell Dev Biol. 9: 601929. PMID: 33996789
  6. 在预防ALS中抑制细胞外调节激酶（ERK）-1/2信号通路：靶向抑制剂及其对神经功能障碍的影响。  |  Sahu, R., et al. 2021. Eur J Cell Biol. 100: 151179. PMID: 34560374
  7. 通过抑制细胞外信号调节激酶（ERK）1/2 通路促进维生素 C 诱导的肌生成  |  Fu, X., et al. 2022. Biochem Biophys Res Commun. 612: 57-62. PMID: 35504090
  8. 在处于高血糖状态的人体心肌细胞中，ERK 1/2通路的抑制作用可下调YAP1/TAZ信号。  |  Joddar, B., et al. 2023. Biochem Biophys Res Commun. 648: 72-80. PMID: 36736094
  9. Rab32通过调节ERK/Drp1介导的线粒体分裂促进胶质母细胞瘤的迁移和侵袭。  |  Chen, P., et al. 2023. Cell Death Dis. 14: 198. PMID: 36922509
  10. 猪β-防御素-2通过ROS-ERK1/2信号通路减轻黄曲霉毒素B1引起的肠粘膜损伤。  |  Li, Q., et al. 2023. Sci Total Environ. 905: 167201. PMID: 37734607

订购信息

产品名称产品编号规格价格数量收藏夹

ERK 1/2 抗体 (MK1)

sc-135900
200 µg/ml
RMB2377.00

ERK 1/2 (MK1): m-IgG Fc BP-HRP 套装

sc-539888
200 µg Ab; 10 µg BP
RMB2662.00

ERK 1/2 (MK1): m-IgG1 BP-HRP 套装

sc-541765
200 µg Ab; 20 µg BP
RMB2662.00

Can ERK2 (SC-135900) be used as a loading control for western blot lysates?

Asked by: Eldhose
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Tech Service 1
Date published: 2021-02-02

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Anonymous
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2016-12-22

Will this ERK1/2 antibody detect the protein in bovine samples?

Asked by: Anonymous
Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2016-12-22
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Rated 4 out of 5 by from sc-135900 ERK1/2 antibodyWe use this antibody in western blot for ERK1/2 detection in HUVEC cells.
Date published: 2023-07-07
Rated 5 out of 5 by from Astrocytes from cortex and striatum show differentAstrocytes from cortex and striatum show differential responses to mitochondrial toxin and BDNF: implications for protection of striatal neurons expressing mutant huntingtin
Date published: 2022-05-30
Rated 5 out of 5 by from SK030204V100ERK 1/2 抗体 (MK1)，抗体很好，实验很顺利，老板很满意。。下次还是买这个品牌的抗体。建议你也可以买这个
Date published: 2022-02-07
Rated 5 out of 5 by from ExcellentExcellent bands and clear film. Incubated in 3% v/w milk overnight.
Date published: 2021-03-17
Rated 5 out of 5 by from Excellent antibody for WBsI have used this antibody at 1:1000 dilution for western blotting using a number of human cell lines and obtained a very strong signal. Extremely pleased with this antibody.
Date published: 2019-06-15
Rated 1 out of 5 by from No signal in In-Cell WesternThis antibody did not work for my In-Cell Western assays in HEK293. I use 4% paraformaldehyde fixation and methanol permeabilization, and I don't know if it could work with a different protocol. I would not purchase this antibody for any immunocytochemistry approaches.
Date published: 2018-05-07
Rated 5 out of 5 by from good resultThis antibody can be used to publish using the antibody in WB and IHC in mouse neuron.
Date published: 2017-05-28
Rated 5 out of 5 by from goodERK 1/2 antibody worked well in the rat cerebrum tissue for WB analysis
Date published: 2017-05-13
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ERK 1/2 抗体 (MK1) is rated 4.4 out of 5 by 18.
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