Date published: 2025-9-8
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Erg-1/2/3 Antibody (C-1) - Western Blotting - Image 380642
Erg-1/2/3 抗体 (C-1): sc-376293. A-431 核提取物 (A), HUV-EC-C (B) 和 ECV304 (C) 全细胞裂解液中 Erg-1/2/3 表达的 Western 印迹分析.
Erg-1/2/3 抗体 (C-1): sc-376293. A-431 核提取物 (A), HUV-EC-C (B) 和 ECV304 (C) 全细胞裂解液中 Erg-1/2/3 表达的 Western 印迹分析.

Erg-1/2/3 抗体 (C-1): sc-376293

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说明书
  • Erg-1/2/3抗体(C-1)是小鼠单克隆IgG1 κ, 在13篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列26-120位于human物种的Erg-1的N-terminus附近
  • 抗-Erg-1/2/3 抗体 (C-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Erg-1, Erg-2 and Erg-3
  • 抗Erg-1/2/3抗体（C-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-376293 X, 200 µg/0.1 ml)
  • 关于如何获取Erg-1/2/3 (C-1): sc-376293的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 是 Erg-1/2/3 抗体 (C-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Erg-1/2/3 抗体 (C-1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Erg 抗体包括 Erg-1, Erg-2 和 Erg-3

Erg-1/2/3抗体（C-1）是一种IgG1 κ小鼠单克隆Erg-1/2/3抗体（也被称作Erg-1/2/3抗体或ERG（ETS相关基因）抗体），可通过WB, IP, IF, IHC(P) 和 ELISA检测小鼠、大鼠和人类来源的Erg-1、Erg-2和Erg-3。Erg-1/2/3抗体（C-1）既有非偶联形式的抗Erg-1/2/3抗体，也有多种偶联形式的抗Erg-1/2/3抗体，包括琼脂糖、HRP、PE、FITC以及多种Alexa Fluor®偶联物。Ets-1是基因家族的原型成员，这个基因家族的确定是基于与从E26成红细胞增多症病毒中分离出的v-Ets癌基因的同源性。目前，该基因家族包括Ets-1、Ets-2、Erg-1-3、Elk-1、Elf-1、Elf-5、NERF、PU.1、PEA3、ERM、FEV、ER8l、Fli-1、TEL、Spi-B、ESE-1、ESE-3A、Net、ABT1和ERF。Ets基因家族的成员表现出不同的组织表达模式，并共享一个高度保守的羧基末端结构域，该结构域含有一个与SV40大T抗原核定位信号序列相关的序列。这个保守结构域对Ets-1与DNA的结合至关重要，并可能是Ets基因家族所有成员DNA结合活性的原因。这些蛋白中的几种已被证明能识别DNA中的相似基序，这些基序共享一个位于中央的5'-GGAA-3'元件。由于选择性剪接和起始密码子的选择性使用，Erg基因可编码多种蛋白。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Erg-1/2/3 抗体 (C-1)

sc-376293
200 µg/ml
RMB2377.00

Erg-1/2/3 (C-1): m-IgG Fc BP-HRP 套装

sc-526021
200 µg Ab; 10 µg BP
RMB2662.00

Erg-1/2/3 抗体 (C-1) X

sc-376293 X
200 µg/0.1 ml
RMB2377.00

Erg-1/2/3 抗体 (C-1) AC

sc-376293 AC
500 µg/ml, 25% agarose
RMB3129.00

Erg-1/2/3 抗体 (C-1) HRP

sc-376293 HRP
200 µg/ml
RMB2377.00

Erg-1/2/3 抗体 (C-1) FITC

sc-376293 FITC
200 µg/ml
RMB2482.00

Erg-1/2/3 抗体 (C-1) PE

sc-376293 PE
200 µg/ml
RMB2580.00

Erg-1/2/3 抗体 (C-1) Alexa Fluor® 488

sc-376293 AF488
200 µg/ml
RMB2685.00

Erg-1/2/3 抗体 (C-1) Alexa Fluor® 546

sc-376293 AF546
200 µg/ml
RMB2685.00

Erg-1/2/3 抗体 (C-1) Alexa Fluor® 594

sc-376293 AF594
200 µg/ml
RMB2685.00

Erg-1/2/3 抗体 (C-1) Alexa Fluor® 647

sc-376293 AF647
200 µg/ml
RMB2685.00

Erg-1/2/3 抗体 (C-1) Alexa Fluor® 680

sc-376293 AF680
200 µg/ml
RMB2685.00

Erg-1/2/3 抗体 (C-1) Alexa Fluor® 790

sc-376293 AF790
200 µg/ml
RMB2685.00

Do you have a suitable isotype control for this Ab (Santa Cruz Biotechnology sc-376293, in AF647)? We use it for ICF (Intracellular Flow) and would like to evaluate what the background signal = no ERG expression level is, so an isotype ctrl would be good.

Asked by: Customer
Thank you for your question. sc-3877 normal mouse IgG1 is recommended for flow cytometry. https://www.scbt.com/p/normal-mouse-igg1
Answered by: BlakeJ
Date published: 2024-09-05

Do you have an ERG antibody that can detect endothelium in tissue from rabbits? 

Asked by: Paul
We only have ERG antibodies that detect mouse, rat, and human samples.
Answered by: Technical Service
Date published: 2024-03-28

Is it suitable for mouse tissue, eg. retina IF application?

Asked by: liqf
This primary antibody is recommended and warrantied for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-06-16

When should the TransCruz reagent sc-376293 X be used instead of the standard form sc-376293?

Asked by: jenni
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Tech Service 3
Date published: 2017-01-27
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Rated 5 out of 5 by from the ERG stained beautifullyEven after harsh retrieval conditions for achieving BrdU staining, the ERG stained beautifully.
Date published: 2017-02-11
Rated 5 out of 5 by from Great Erg-1/2/3 monoclonal ab for WB and IHC(P)Strong clean band in A-431 nuclear extract and beautiful nuclear localized Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue.
Date published: 2016-12-23
Rated 5 out of 5 by from works very well at detecting endogenousworks very well at detecting endogenous ERG expression in human umbilical vein endothelial cells
Date published: 2015-07-22
Rated 5 out of 5 by from Produced excellent immunoperoxidase nuclearProduced excellent immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human spleen tissue. -SCBT QC
Date published: 2015-03-04
Rated 5 out of 5 by from Large amount of signal detected in WB in human ErgLarge amount of signal detected in WB in human Erg-1/2/3 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-02-20
Rated 5 out of 5 by from Great specificity in WB in AGreat specificity in WB in A-431 nuclear extract. -SCBT QC
Date published: 2015-02-18
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Erg-1/2/3 抗体 (C-1) is rated 5.0 out of 5 by 6.
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