Date published: 2025-9-6
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epsin 1 Antibody (C-11) - Western Blotting - Image 355710
epsin 1 抗体 (C-11): sc-55556. K-562 (A), Hep G2 (B) 和 KNRK (C) 全细胞裂解液中 epsin 1 表达的 Western 印迹分析.
epsin 1 抗体 (C-11): sc-55556. K-562 (A), Hep G2 (B) 和 KNRK (C) 全细胞裂解液中 epsin 1 表达的 Western 印迹分析.

epsin 1 抗体 (C-11): sc-55556

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说明书
  • epsin 1 抗体 C-11 是小鼠单克隆 IgG2a κ，epsin 1抗体, 在4篇文献中引用，规格为200 µg/ml
  • 免疫human物种的epsin 1的氨基酸311-440
  • epsin 1 抗体 (C-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 epsin 1
  • 抗epsin 1抗体（C-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取epsin 1 (C-11): sc-55556的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 epsin 1 抗体 (C-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与epsin 1 抗体 (C-11) 组合成套装提供。 (请参阅下面的订购信息)。

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说明书与实验方案
研究信息

関連項目

此外 epsin 抗体 包括 epsin 1, epsin 2和epsin 4

Epsin 1 抗体（C-11）是一种小鼠单克隆 IgG2a kappa 轻链抗体，可通过免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附试验（ELISA）检测小鼠、大鼠和人源的 Epsin 1 蛋白。抗表素 1 抗体 (C-11) 有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。Epsin 1 (EPN1) 在内吞膜过程中起着至关重要的作用，尤其是在形成 Clathrin 包裹囊泡的过程中，而 Clathrin 包裹囊泡对于膜蛋白和脂质的内吞至关重要。EPN1 主要位于囊泡包被凹坑的前缘，EPN1 在这里促进膜弯曲，这是内吞中的一个关键步骤。这种定位可确保细胞外物质和信号分子的有效吸收，从而影响细胞通讯和营养吸收。EPN1 还与 Eps15 和 Clathrin 等关键蛋白相互作用，EPN1 的功能受翻译后修饰（包括磷酸化）的调控，磷酸化可在有丝分裂等不同细胞状态下调节其活性。了解 EPN1 的动态和相互作用对于阐明各种生理和病理过程中的内吞机制和影响至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

epsin 1 抗体 (C-11) 参考文献:

  1. Epsin 与 POB1 的 EH 结构域结合，并调节受体介导的内吞作用。  |  Morinaka, K., et al. 1999. Oncogene. 18: 5915-22. PMID: 10557078
  2. epsins定义了一个与clathrin外壳成分相互作用的蛋白质家族，并包含一个新的蛋白质模块。  |  Rosenthal, JA., et al. 1999. J Biol Chem. 274: 33959-65. PMID: 10567358
  3. Epsin通过直接与clathrin末端结构域结合而与clathrin结合。有证据表明，它们通过两个不同的位点进行合作性结合。  |  Drake, MT., et al. 2000. J Biol Chem. 275: 6479-89. PMID: 10692452
  4. 泛素连接基序以内细胞适配蛋白epsin为目标进行泛素化。  |  Oldham, CE., et al. 2002. Curr Biol. 12: 1112-6. PMID: 12121618
  5. 由epsin驱动的凝集素包被坑的弯曲。  |  Ford, MG., et al. 2002. Nature. 419: 361-6. PMID: 12353027
  6. Epsins：内吞过程中的适配器？  |  Wendland, B. 2002. Nat Rev Mol Cell Biol. 3: 971-7. PMID: 12461563
  7. AP180 N-terminal homology (ANTH) 和 Epsin N-terminal homology (ENTH) 结构域不同的膜相互作用机制。  |  Stahelin, RV., et al. 2003. J Biol Chem. 278: 28993-9. PMID: 12740367
  8. Epsin N-terminal homology/AP180 N-terminal homology (ENTH/ANTH) 结构域在管蛋白结合中的作用。  |  Hussain, NK., et al. 2003. J Biol Chem. 278: 28823-30. PMID: 12750376
  9. 破伤风毒素的内化是通过在脂质微域内启动的顺序性凝集素依赖机制进行的，与epsin1无关。  |  Deinhardt, K., et al. 2006. J Cell Biol. 174: 459-71. PMID: 16880274
  10. EPSIN1 可调节血浆膜上 FLAGELLIN SENSING2 的丰度，从而产生有效的免疫反应。  |  Collins, CA., et al. 2020. Plant Physiol. 182: 1762-1775. PMID: 32094305
  11. Epsin 是一种与凝集素介导的内吞作用有关的 EH 域结合蛋白。  |  Chen, H., et al. 1998. Nature. 394: 793-7. PMID: 9723620

订购信息

产品名称产品编号规格价格数量收藏夹

epsin 1 抗体 (C-11)

sc-55556
200 µg/ml
RMB2377.00

epsin 1 (C-11): m-IgG Fc BP-HRP 套装

sc-525638
200 µg Ab; 10 µg BP
RMB2662.00

epsin 1 (C-11): m-IgG2a BP-HRP 套装

sc-546346
200 µg Ab; 10 µg BP
RMB2662.00

epsin 1 抗体 (C-11) AC

sc-55556 AC
500 µg/ml, 25% agarose
RMB3129.00

epsin 1 抗体 (C-11) HRP

sc-55556 HRP
200 µg/ml
RMB2377.00

epsin 1 抗体 (C-11) FITC

sc-55556 FITC
200 µg/ml
RMB2482.00

epsin 1 抗体 (C-11) PE

sc-55556 PE
200 µg/ml
RMB2580.00

epsin 1 抗体 (C-11) Alexa Fluor® 488

sc-55556 AF488
200 µg/ml
RMB2685.00

epsin 1 抗体 (C-11) Alexa Fluor® 546

sc-55556 AF546
200 µg/ml
RMB2685.00

epsin 1 抗体 (C-11) Alexa Fluor® 594

sc-55556 AF594
200 µg/ml
RMB2685.00

epsin 1 抗体 (C-11) Alexa Fluor® 647

sc-55556 AF647
200 µg/ml
RMB2685.00

epsin 1 抗体 (C-11) Alexa Fluor® 680

sc-55556 AF680
200 µg/ml
RMB2685.00

epsin 1 抗体 (C-11) Alexa Fluor® 790

sc-55556 AF790
200 µg/ml
RMB2685.00

Can epsin 1 (C-11): sc-55556 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Trav11
Thank you for your question. Yes, epsin 1 (C-11): sc-55556 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Works for WBI got a sample Ab and worked for WB with 1:200 dil. Specific sharp band was obtained from this Ab compared to two other companies' Abs. Recognized endogenous protein, IP'd protein and recombinant protein as well. I bought a full vial. IP did not work well with this Ab but we just need to use more Abs than recommended. Highly recommend.
Date published: 2018-04-16
Rated 5 out of 5 by from Great for WBI got sample antibody just to test Tiam1 in HEK cells after over expression of two different cell surface receptors. This antibody is so specific. and I got a clear sharp band. I will be ordering this monoclonal antibody for my further experiments.
Date published: 2017-12-06
Rated 4 out of 5 by from goodIt's a sample gift from Santa, I tested it by IF in MDCK cells. We observed discrete dots at periphery of cells, however, there was a little unspecific staining of nucleus.
Date published: 2017-10-07
Rated 5 out of 5 by from Good clathrin markerWorked well at a 1:50 dilution. Strong colocalization with clathrin coated vesicles
Date published: 2017-08-29
Rated 5 out of 5 by from Good qualityIt works well at 1:2000 dilution. We used it both on endogenous Epsin1 and overexpressed mice Epsin1.
Date published: 2016-12-20
Rated 5 out of 5 by from Good specificity in WB in human transfectedGood specificity in WB in human transfected 293T and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-04-14
Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmicExceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human rectum tissue. -SCBT QC
Date published: 2015-03-25
Rated 5 out of 5 by from Positive signal in WB in mouse transfectedPositive signal in WB in mouse transfected 293T and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-03-21
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epsin 1 抗体 (C-11) is rated 4.9 out of 5 by 8.
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