Date published: 2025-9-7

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Ep-CAM 抗体 (EBA-1): sc-66020

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说明书
  • Ep-CAM抗体(EBA-1)是小鼠单克隆IgG1 λ, 在29篇文献中引用,规格为200 µg/ml
  • 在human来源的乳腺癌相关粘蛋白BCA-225的大分子聚集物上
  • 抗-Ep-CAM 抗体 (EBA-1) 推荐用于 WB, IP, IF, IHC(P) 和 FCM,检测mouse, rat 和human 来源的 Ep-CAM
  • 抗Ep-CAM抗体(EBA-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ep-CAM (EBA-1): sc-66020免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 Ep-CAM 抗体 (EBA-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Ep-CAM 抗体 (EBA-1) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Ep-CAM 抗体(EBA-1)是一种小鼠单克隆 IgG1 抗体,可通过免疫印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和流式细胞术(FCM)检测小鼠、大鼠和人的上皮细胞粘附分子(Ep-CAM)。Ep-CAM (EBA-1) 抗体有非共轭型和多种共轭型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。上皮细胞粘附分子又称肿瘤相关钙信号转导子 1 和 MK-1,是一种重要的单体膜糖蛋白,主要表达于正常人的上皮组织和各种癌症。人类 Ep-CAM 基因编码一种 314 个氨基酸的蛋白质,它有两种形式,一种是主要变体,另一种是次要变体,后者在使用氨基糖基化抑制剂妥卡霉素处理后会减少。Ep-CAM 在细胞-细胞粘附中发挥着重要作用,而细胞-细胞粘附对于维持组织完整性和调节细胞信号通路至关重要。Ep-CAM在多种癌症中的过度表达使该蛋白成为人类实体瘤可视化和治疗策略的一个有希望的靶点。Ep-CAM 的结构包括一个具有两个表皮生长因子样重复序列的胞外结构域,其后是一个半胱氨酸贫乏区,而半胱氨酸贫乏区对粘附性至关重要,因此 Ep-CAM 在正常生理和癌症病理中都非常重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Ep-CAM 抗体 (EBA-1)

sc-66020
200 µg/ml
RMB2377.00

Ep-CAM (EBA-1): m-IgG Fc BP-HRP 套装

sc-526975
200 µg Ab; 10 µg BP
RMB2662.00

Ep-CAM (EBA-1): m-IgG1 BP-HRP 套装

sc-532348
200 µg Ab; 20 µg BP
RMB2662.00

Ep-CAM 抗体 (EBA-1) AC

sc-66020 AC
500 µg/ml, 25% agarose
RMB3129.00

Ep-CAM 抗体 (EBA-1) HRP

sc-66020 HRP
200 µg/ml
RMB2377.00

Ep-CAM 抗体 (EBA-1) FITC

sc-66020 FITC
200 µg/ml
RMB2482.00

Ep-CAM 抗体 (EBA-1) PE

sc-66020 PE
200 µg/ml
RMB2580.00

Ep-CAM 抗体 (EBA-1) Alexa Fluor® 488

sc-66020 AF488
200 µg/ml
RMB2685.00

Ep-CAM 抗体 (EBA-1) Alexa Fluor® 546

sc-66020 AF546
200 µg/ml
RMB2685.00

Ep-CAM 抗体 (EBA-1) Alexa Fluor® 594

sc-66020 AF594
200 µg/ml
RMB2685.00

Ep-CAM 抗体 (EBA-1) Alexa Fluor® 647

sc-66020 AF647
200 µg/ml
RMB2685.00

Ep-CAM 抗体 (EBA-1) Alexa Fluor® 680

sc-66020 AF680
200 µg/ml
RMB2685.00

Ep-CAM 抗体 (EBA-1) Alexa Fluor® 790

sc-66020 AF790
200 µg/ml
RMB2685.00

If I want to use EV sample to do western blot, how much antibody should be add to my sample? what should be used for diluting the antibody?

Asked by: Shirley123
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-02-09

what should be used to dilute the antibody?

Asked by: jane29
Thank you for your question. The antibody should be diluted in our UltraCruz® Blocking Reagent: sc-516214 or in TBS Blotto A: sc-2333.
Answered by: Tech Support Europe
Date published: 2022-02-04

How well is this Ab validated for the use in the rat species? We are trying to find an Ab for looking at EpCAM on rat epithelial cells by flow cytommetry.

Asked by: JuWei
Thank you for your question. We do not currently have data using this antibody in any rat cell lines, but the antibody is recommended for use in rat based on product citations in mouse and rat samples. One example of a product citation in rat tissue specifically can be found on PubMed with PMID: 22093411.
Answered by: Technical Support
Date published: 2020-07-08

what is the exact epitope recognized by this antibody?

Asked by: raniagh
Thank you for your question. This antibody was raised against macromolecular aggregates containing breast carcinoma-associated mucin BCA-225 of human origin. The exact epitope has not been mapped, so no further information is available.
Answered by: Tech Service
Date published: 2020-02-11

Is Ep-CAM Antibody (EBA-1): sc-66020 available directly conjugated to HRP or fluorescent dyes?

Asked by: jenni
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at [email protected] Alternatively, consider using Ep-CAM (C-10), which is available conjugated to agarose, HRP, FITC, PE, and more. (https://www.scbt.com/scbt/product/ep-cam-antibody-c-10)
Answered by: Technical Support 6
Date published: 2017-01-09
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Rated 5 out of 5 by from Very good antibodyWorks as we expected. Tested by immunofluorescence. Great
Date published: 2018-12-18
Rated 5 out of 5 by from Outstanding IHC !!Very pleased with IHC data; clear, specific, very nice
Date published: 2017-01-10
Rated 5 out of 5 by from Especially good for IHCStrong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples. Well-cited for use in IHC.
Date published: 2016-12-22
Rated 5 out of 5 by from Good bands in WBGood bands in WB, happy with purchase
Date published: 2015-04-30
Rated 5 out of 5 by from WBWB, IF(pfa) and FCM publication using rat and human liver samples. -SCBT Publication Review
Date published: 2015-04-26
Rated 5 out of 5 by from Produced superb immunoperoxidase membraneProduced superb immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human colon tissue. -SCBT QC
Date published: 2015-03-18
Rated 5 out of 5 by from IF publication using primary human gingivalIF publication using primary human gingival epithelial cells and human gingival fibroblasts -SCBT Publication Review
Date published: 2015-02-22
Rated 5 out of 5 by from ParaffinParaffin-embedded IHC publication using human liver samples -SCBT Publication Review
Date published: 2015-01-09
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Ep-CAM 抗体 (EBA-1) is rated 5.0 out of 5 by 8.
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