Date published: 2025-9-7
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EMR1 Antibody (D-11) - Western Blotting - Image 376786
EMR1 抗体 (D-11): sc-365340. K-562 (A), THP-1 (B), TF-1 (C), SUP-T1 (D), TK-1 (E) 和 J774. A1 (F) 全细胞裂解液中 EMR1 表达的 Western 印迹分析.
EMR1 抗体 (D-11): sc-365340. K-562 (A), THP-1 (B), TF-1 (C), SUP-T1 (D), TK-1 (E) 和 J774. A1 (F) 全细胞裂解液中 EMR1 表达的 Western 印迹分析.

EMR1 抗体 (D-11): sc-365340

4.3(3)
寫評論提問

说明书
  • EMR1 抗体 D-11 是小鼠单克隆 IgG2a κ，EMR1抗体, 在4篇文献中引用，规格为200 µg/ml
  • 与human的EMR1的细胞外结构域内的氨基酸241-530杂交
  • EMR1 抗体 (D-11) 推荐用于 WB, IP, IF 和 ELISA，检测 EMR1 of human origin, F4/80 of mouse origin and the corresponding rat homolog
  • 抗EMR1抗体（D-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取EMR1 (D-11): sc-365340的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2a BP-HRP">m-IgG2a BP-HRP是EMR1 Antibody (D-11) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与EMR1 Antibody (D-11) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外EMR and F4/80抗体，包括F4/80

EMR1抗体（D-11）是一种IgG2a κ小鼠单克隆EMR1抗体（也称为粘附G蛋白偶联受体E1抗体、ADGRE1抗体、EMR1抗体、TM7LN3抗体或小鼠F4/80抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的EMR1蛋白。 EMR1抗体（D-11）可以以非偶联抗EMR1抗体形式和多种偶联抗EMR1抗体形式提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。表皮生长因子（EGF）-TM7家族由B类G蛋白偶联受体组成，包括CD97、EMR1（EGF样分子，含有粘蛋白样激素受体1，在小鼠中称为F4/80）、EMR2、EMR3、FIRE和ETL。这些家族成员的特征是具有几个N端EGF结构域的延伸细胞外区域，主要在免疫系统细胞上表达。 EGF-TM7蛋白家族由人类染色体19p13上的基因簇编码。F4/80分子仅在巨噬细胞表面表达，并作为成熟巨噬细胞组织的标记，包括肝脏的库普弗细胞、脾红髓巨噬细胞、脑小胶质细胞、肠道固有层和皮肤中的朗格罕氏细胞。 F4/80/EMR1进行广泛的N-连接糖基化和一些O-连接糖基化。 F4/80/EMR1的功能尚不清楚，但据推测它参与巨噬细胞的粘附事件、细胞迁移或作为巨噬细胞的G蛋白偶联信号成分。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

EMR1 抗体 (D-11) 参考文献:

  1. 人类 EMR2 是染色体 19p13.1 上的一种新型 EGF-TM7 分子，与 CD97 关系密切。  |  Lin, HH., et al. 2000. Genomics. 67: 188-200. PMID: 10903844
  2. 人类 EGF-TM7 家族成员 EMR2 是一种表达于髓系细胞的异源二聚体受体。  |  Kwakkenbos, MJ., et al. 2002. J Leukoc Biol. 71: 854-62. PMID: 11994511
  3. 小鼠种系中的 F4/80 糖蛋白失活。  |  Schaller, E., et al. 2002. Mol Cell Biol. 22: 8035-43. PMID: 12391169
  4. EMR1是激素受体家族中一个不寻常的成员，具有七个跨膜片段。  |  Baud, V., et al. 1995. Genomics. 26: 334-44. PMID: 7601460
  5. 巨噬细胞表面糖蛋白 F4/80 是一种高度糖基化的蛋白聚糖。  |  Haidl, ID. and Jefferies, WA. 1996. Eur J Immunol. 26: 1139-46. PMID: 8647179
  6. 出生后大鼠脑垂体小胶质细胞和巨噬细胞的发育  |  Mander, TH. and Morris, JF. 1996. Cell Tissue Res. 286: 347-55. PMID: 8929337

订购信息

产品名称产品编号规格价格数量收藏夹

EMR1 抗体 (D-11)

sc-365340
200 µg/ml
RMB2377.00

EMR1 (D-11): m-IgG2a BP-HRP 套装

sc-545938
200 µg Ab; 10 µg BP
RMB2662.00

EMR1 抗体 (D-11) AC

sc-365340 AC
500 µg/ml, 25% agarose
RMB3129.00

EMR1 抗体 (D-11) HRP

sc-365340 HRP
200 µg/ml
RMB2377.00

EMR1 抗体 (D-11) FITC

sc-365340 FITC
200 µg/ml
RMB2482.00

EMR1 抗体 (D-11) PE

sc-365340 PE
200 µg/ml
RMB2580.00

EMR1 抗体 (D-11) Alexa Fluor® 488

sc-365340 AF488
200 µg/ml
RMB2685.00

EMR1 抗体 (D-11) Alexa Fluor® 546

sc-365340 AF546
200 µg/ml
RMB2685.00

EMR1 抗体 (D-11) Alexa Fluor® 594

sc-365340 AF594
200 µg/ml
RMB2685.00

EMR1 抗体 (D-11) Alexa Fluor® 647

sc-365340 AF647
200 µg/ml
RMB2685.00

EMR1 抗体 (D-11) Alexa Fluor® 680

sc-365340 AF680
200 µg/ml
RMB2685.00

EMR1 抗体 (D-11) Alexa Fluor® 790

sc-365340 AF790
200 µg/ml
RMB2685.00

Anti-EMR1 Antibody (D-11): sc-365340 was tested for western blot? Do you sell positive and negative control?

Asked by: Anonymous
Thank you for your question. Anti-EMR1 Antibody (D-11): sc-365340 is recommended for detection of EMR1 of human origin, F4/80 of mouse origin and the corresponding rat homolog by WB. We offer the following Positive Controls for this antibody: TF-1 cell lysate: sc-2412, K-562 whole cell lysate: sc-2203, or J774.A1 cell lysate: sc-3802.
Answered by: Tech Support Europe
Date published: 2021-11-04

国内多久到货

Asked by: 幸运XX
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

I want to enqiure about F4/80 with reactivity to Rat and useful for staining purposes.

Asked by: Ankita Srivastava
Thank you for your question. We currently do not have any data or product citations in rat samples, but this antibody is recommended and warrantied for detection of the F4/80 homolog in rat based on reactivity with mouse.
Answered by: Technical Support
Date published: 2019-09-11

What blocking solution should I use with EMR1 (D-11): sc-365340 monoclonal antibody for Western blot?

Asked by: Germaine
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-28
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Rated 4 out of 5 by from Excellent AntibodyGives a sharp, single band at the correct Molecular Weight. The signal is not very strong but is good enough.
Date published: 2018-01-03
Rated 4 out of 5 by from Works in IHCGood signal in mouse tissue by IHC with some backgrounds
Date published: 2017-08-28
Rated 5 out of 5 by from Produced nice Western blot data of EMR1 expressionProduced nice Western blot data of EMR1 expression in EOC 20 whole cell lysate. -SCBT QC
Date published: 2014-01-14
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EMR1 抗体 (D-11) is rated 4.3 out of 5 by 3.
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