Date published: 2025-9-7
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elastin Antibody (BA-4) - Western Blotting - Image 129605
弹性蛋白抗体 (BA-4): sc-58756. 小鼠肝组织提取物中弹性蛋白表达的 Western 印迹分析.
elastin Antibody (BA-4) - Western Blotting - Image 129605
弹性蛋白抗体 (BA-4): sc-58756. 小鼠肝组织提取物中弹性蛋白表达的 Western 印迹分析.
elastin Antibody (BA-4) - Immunohistochemistry - Image 157174
弹性蛋白抗体 (BA-4): sc-58756. 福尔马林固定, 石蜡包埋的人睾丸组织的免疫过氧化物酶染色, 显示细胞外纤维和曲细精管细胞膜染色.
弹性蛋白抗体 (BA-4): sc-58756. 小鼠肝组织提取物中弹性蛋白表达的 Western 印迹分析.

elastin 抗体 (BA-4): sc-58756

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寫評論提問

说明书
  • elastin 抗体 BA-4 是小鼠单克隆 IgG1 κ，elastin抗体, 在36篇文献中引用，规格为200 µg/ml
  • 免疫bovine物种的elastin
  • elastin 抗体 (BA-4) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 insoluble elastin, α-elastin and soluble non-cross linked precursor of elastin (tropoelastin); 也和以下物种反应，包括： 和 bovine, porcine, feline and canine
  • 抗elastin抗体（BA-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取elastin (BA-4): sc-58756的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 elastin 抗体 (BA-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与elastin 抗体 (BA-4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

弹性蛋白抗体（BA-4）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠和人类来源的弹性蛋白。抗弹性蛋白抗体（BA-4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。弹性蛋白在提供结缔组织的弹性和恢复力方面发挥着至关重要的作用，特别是在动脉细胞外基质中，弹性蛋白对于维持血管的完整性和功能至关重要。弹性蛋白的独特结构以高含量的甘氨酸、脯氨酸和疏水残基为特征，使其能够通过赖氨酸衍生的交联（如脱氨酶）形成类似橡胶的网络。这种结构特性对于组织伸展和回弹的能力至关重要，对于承受巨大机械应力的器官（如肺和血管）尤其重要。随着年龄的增长，弹性蛋白与胶原蛋白的交联会导致组织弹性下降，引发各种与年龄相关的疾病。此外，弹性蛋白缺乏还与严重疾病有关，如主动脉瓣狭窄和威廉姆斯-博伊伦综合征，这凸显了弹性蛋白在心血管健康中的重要性。人类弹性蛋白基因位于7q11.23染色体上，这强调了弹性蛋白在发育和病理学背景中的遗传意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

elastin 抗体 (BA-4) 参考文献:

  1. 人类弹性蛋白基因：定位到 7 号染色体长臂的新证据。  |  Fazio, MJ., et al. 1991. Am J Hum Genet. 48: 696-703. PMID: 2014796
  2. 基质金属蛋白酶与胶原蛋白和弹性蛋白的相互作用  |  Van Doren, SR. 2015. Matrix Biol. 44-46: 224-31. PMID: 25599938
  3. 弹性蛋白、动脉力学和心血管疾病。  |  Cocciolone, AJ., et al. 2018. Am J Physiol Heart Circ Physiol. 315: H189-H205. PMID: 29631368
  4. 生物医学应用中的弹性蛋白样多肽。  |  Varanko, AK., et al. 2020. Annu Rev Biomed Eng. 22: 343-369. PMID: 32343908
  5. 肿瘤微环境中的弹性蛋白  |  Wang, Y., et al. 2020. Adv Exp Med Biol. 1272: 1-16. PMID: 32845499
  6. 弹性蛋白基材料的纤维支架。  |  Rodriguez-Cabello, JC., et al. 2021. Front Bioeng Biotechnol. 9: 652384. PMID: 34336798
  7. 弹性蛋白转录调控的新机制。  |  Procknow, SS. and Kozel, BA. 2022. Am J Physiol Cell Physiol. 323: C666-C677. PMID: 35816641
  8. 弹性蛋白样多肽的细胞因子共轭物。  |  Gong, L., et al. 2022. Adv Drug Deliv Rev. 190: 114541. PMID: 36126792
  9. 正常和患病人体主动脉弹性蛋白中含有脱丝氨酸（s）的多肽部分的分离和表征。  |  Henin-Pizieux, O., et al. 1979. Paroi Arterielle. 5: 41-53. PMID: 492780
  10. 发育障碍--威廉姆斯综合症中弹性蛋白基因座的半杂合子。  |  Ewart, AK., et al. 1993. Nat Genet. 5: 11-6. PMID: 7693128
  11. 弹性蛋白基因（ELN）第 30 号外显子的框架移位突变导致的皮肤松弛症。  |  Zhang, MC., et al. 1999. J Biol Chem. 274: 981-6. PMID: 9873040

订购信息

产品名称产品编号规格价格数量收藏夹

elastin 抗体 (BA-4)

sc-58756
200 µg/ml
RMB2377.00

elastin (BA-4): m-IgG Fc BP-HRP 套装

sc-526912
200 µg Ab; 10 µg BP
RMB2662.00

elastin (BA-4): m-IgG1 BP-HRP 套装

sc-532285
200 µg Ab; 20 µg BP
RMB2662.00

elastin 抗体 (BA-4) AC

sc-58756 AC
500 µg/ml, 25% agarose
RMB3129.00

elastin 抗体 (BA-4) HRP

sc-58756 HRP
200 µg/ml
RMB2377.00

elastin 抗体 (BA-4) FITC

sc-58756 FITC
200 µg/ml
RMB2482.00

elastin 抗体 (BA-4) PE

sc-58756 PE
200 µg/ml
RMB2580.00

elastin 抗体 (BA-4) Alexa Fluor® 488

sc-58756 AF488
200 µg/ml
RMB2685.00

elastin 抗体 (BA-4) Alexa Fluor® 546

sc-58756 AF546
200 µg/ml
RMB2685.00

elastin 抗体 (BA-4) Alexa Fluor® 594

sc-58756 AF594
200 µg/ml
RMB2685.00

elastin 抗体 (BA-4) Alexa Fluor® 647

sc-58756 AF647
200 µg/ml
RMB2685.00

elastin 抗体 (BA-4) Alexa Fluor® 680

sc-58756 AF680
200 µg/ml
RMB2685.00

elastin 抗体 (BA-4) Alexa Fluor® 790

sc-58756 AF790
200 µg/ml
RMB2685.00

We used this antibody (1:500) for a western blot. We could see bands at about 30 kDa and 25 kDa and weak bands at 20 and 15 kDa. We could see that other research groups have the same problem. Are there any ideas why?

Asked by: Ma_Av_Ol
Thank you for your question. Please contact technical service directly for troubleshooting advice on your experiment.
Answered by: Tech Support Europe
Date published: 2023-02-06

For western blotting, this antibody gives a strong band ~30kDa (expected size is 60-70kDa) in human smooth muscle cell lysates. Do you recommend to use this antibody in non-reducing conditions and perhaps PAGE gel?

Asked by: jdave
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Tech Service 1
Date published: 2022-03-25

We want to use this antigen for elastin detection in mouse (formalin-fixed, paraffin-embedded tissue sections). Do I need to epitope retrieval ？ In that case,　Would you please tell me optimum conditions for epitope retrieval.

Asked by: AH2019
Thank you for your question. We do recommend performing antigen retrieval when using this antibody on formalin-fixed paraffin-embedded tissue sections. We recommend using heat treatment with 10 mM sodium citrate buffer (pH 6.0). Further details about the treatment can be found in our protocol below. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2022-06-14

Does this mouse monoclonal work for IHC or IF staining of mouse tissue?

Asked by: Anna99
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody for elastin is available conjugated to HRP (sc-58756 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; and to either phycoerythrin (sc-58756 PE) or fluoroscein (sc-58756 FITC), Alexa Fluor® 488 (sc-58756 AF488) or Alexa Fluor® 647 (sc-58756 AF647), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Technical Support 12
Date published: 2022-06-14
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Rated 5 out of 5 by from 条带很漂亮特异性很好，跑出的条带很漂亮，两条并排条带，一亮一暗，并没有杂带出现，小鼠皮肤组织，40ug，1:500
Date published: 2018-10-10
Rated 4 out of 5 by from GREATI used it for Immunohistochemistry 1:50, it works well.
Date published: 2017-10-05
Rated 1 out of 5 by from Nonspecific antibodyAntibody has lots of background noise/bands, makes it difficult to identify correct band. Fairly faint result despite doubling protein and using a 1:200 dilution as recommended. Antibody should fall between ladder bands 3 and 4 (98 and 64 kDa) according to reported MW of 71 kDa. Strongest band shows around ~50 kDa, but faint bands also present around that range.
Date published: 2017-08-15
Rated 5 out of 5 by from excellent performaceWe always purchase this antibody use for pig and dog blood vessel cryosections immunofluorescence stain. We got very clear and significant signals.
Date published: 2017-05-19
Rated 5 out of 5 by from obtained clear bandsThe best antibody for western blot of mouse liver tissue extract
Date published: 2017-05-14
Rated 5 out of 5 by from goodI used this antibody to detect the elastin in mouse liver tissue extract, the band is clear.
Date published: 2017-04-25
Rated 4 out of 5 by from Worked on IHC of Frozen Human SamplesSpecific signal of elastin fibers in human pericardium. Tissue fixed in 2% PFA, frozen and cryosectioned into 5 μm slices. Dilution: 1:50.
Date published: 2017-03-03
Rated 5 out of 5 by from Good elastin detectionThis antibody provides strong and clear elastin detection in mouse, rat, or human samples. This product has been featured in several journal publications.
Date published: 2017-01-17
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elastin 抗体 (BA-4) is rated 4.4 out of 5 by 12.
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