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eIF4G Antibody (A-10) - Western Blotting - Image 384066
eIF4G 抗体 (A-10) HRP: sc-133155 HRP. eIF4G 在 Jurkat (A), Neuro-2A (B), A-431 (C), IMR-32 (D) 和 U-251-MG (E) 全细胞裂解液中表达的直接 western 印迹分析.
eIF4G 抗体 (A-10) HRP: sc-133155 HRP. eIF4G 在 Jurkat (A), Neuro-2A (B), A-431 (C), IMR-32 (D) 和 U-251-MG (E) 全细胞裂解液中表达的直接 western 印迹分析.

eIF4G 抗体 (A-10): sc-133155

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寫評論提問

说明书
  • eIF4G 抗体 A-10 是小鼠单克隆 IgG2b κ，eIF4G抗体, 在26篇文献中引用，规格为200 µg/ml
  • 免疫human物种的eIF4G的氨基酸1-300
  • eIF4G 抗体 (A-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 eIF4G
  • 抗eIF4G抗体（A-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取eIF4G (A-10): sc-133155的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 eIF4G 抗体 (A-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与eIF4G 抗体 (A-10) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 eIF 抗体 包括 eIF1, eIF1AY, eIF2 α, eIF2B α, eIF2B β, eIF2B δ, eIF2B ε, eIF2 β, eIF2B γ和eIF2C

eIF4G抗体（A-10）是一种IgG2bκ小鼠单克隆eIF4G抗体（也称为EIF4G1抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人源的eIF4G蛋白。 eIF4G抗体（A-10）可作为非偶联抗eIF4G抗体形式，以及多种偶联形式的抗eIF4G抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。真核细胞中蛋白质合成的起始是由蛋白质起始因子和RNA分子之间的相互作用来调节的。真核起始复合物eIF4F在体外以eIF4G、eIF4E和eIF4A的三聚体复合物存在。该复合物通过诱导mRNA二级结构的解旋来使核糖体结合到mRNA上。 eIF4E在蛋白质合成的早期步骤中与mRNA"帽"结合。 eIF4A作为ATP依赖的RNA解旋酶。 eIF4G作为eIF4E、eIF4A和eIF3复合物之间的桥梁。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

eIF4G 抗体 (A-10) 参考文献:

  1. 蛋白质合成的启动：eIF4G的作用。  |  Prévôt, D., et al. 2003. Biol Cell. 95: 141-56. PMID: 12867079
  2. 人类蛋白质合成启动因子 eIF-4 gamma 的氨基酸序列。  |  Yan, R., et al. 1992. J Biol Chem. 267: 23226-31. PMID: 1429670
  3. 真核生物引发剂-4E 和-4F 既能引发蛋白质合成的 5'帽子依赖型启动，也能引发蛋白质合成的内部启动。  |  Scheper, GC., et al. 1992. J Biol Chem. 267: 7269-74. PMID: 1559971
  4. 翻译中的 RNA 解旋：由真核生物引发剂 eIF-4F 和 eIF-4B 组成的螺旋酶复合体中间体的组装。  |  Jaramillo, M., et al. 1991. Mol Cell Biol. 11: 5992-7. PMID: 1719376
  5. 真核生物翻译起始因子 4A 和 4F 的双向 RNA 螺旋酶活性。  |  Rozen, F., et al. 1990. Mol Cell Biol. 10: 1134-44. PMID: 2304461
  6. 蛋白质合成起始因子 4A 基因的分离和图谱绘制及其在小鼠红细胞白血病细胞分化过程中的表达。  |  Reddy, NS., et al. 1988. Gene. 70: 231-43. PMID: 3215517
  7. 人体组织中 mRNA 盖帽结合蛋白的氨基酸序列。  |  Rychlik, W., et al. 1987. Proc Natl Acad Sci U S A. 84: 945-9. PMID: 3469651
  8. CRISPR-dcas13d-eIF4G翻译调节系统的开发与应用，以抑制草酸钙晶体诱导的肾损伤中的铁死亡。  |  He, Z., et al. 2024. Adv Sci (Weinh). 11: e2309234. PMID: 38380498
  9. hnRNP R促进eIF4G的O-GlcNAc化，并促进轴突蛋白的合成。  |  Zare, A., et al. 2024. Nat Commun. 15: 7430. PMID: 39198412
  10. 用皮卡病毒蛋白酶绘制真核蛋白质合成起始因子 4G（eIF4G）的功能域图。帽子依赖型和帽子非依赖型翻译启动的意义。  |  Lamphear, BJ., et al. 1995. J Biol Chem. 270: 21975-83. PMID: 7665619
  11. 真核蛋白质合成：体外分析。  |  Merrick, WC. 1994. Biochimie. 76: 822-30. PMID: 7880898
  12. eIF4G：翻译的神秘因子开始揭示其秘密。  |  Morley, SJ., et al. 1997. RNA. 3: 1085-104. PMID: 9326485

订购信息

产品名称产品编号规格价格数量收藏夹

eIF4G 抗体 (A-10)

sc-133155
200 µg/ml
RMB2377.00

eIF4G (A-10): m-IgG Fc BP-HRP 套装

sc-528755
200 µg Ab; 10 µg BP
RMB2662.00

eIF4G (A-10): m-IgGκ BP-HRP 套装

sc-521254
200 µg Ab, 40 µg BP
RMB2662.00

eIF4G (A-10): m-IgG2b BP-HRP 套装

sc-550069
200 µg Ab; 10 µg BP
RMB2662.00

eIF4G 抗体 (A-10) AC

sc-133155 AC
500 µg/ml, 25% agarose
RMB3129.00

eIF4G 抗体 (A-10) HRP

sc-133155 HRP
200 µg/ml
RMB2377.00

eIF4G 抗体 (A-10) FITC

sc-133155 FITC
200 µg/ml
RMB2482.00

eIF4G 抗体 (A-10) PE

sc-133155 PE
200 µg/ml
RMB2580.00

eIF4G 抗体 (A-10) Alexa Fluor® 488

sc-133155 AF488
200 µg/ml
RMB2685.00

eIF4G 抗体 (A-10) Alexa Fluor® 546

sc-133155 AF546
200 µg/ml
RMB2685.00

eIF4G 抗体 (A-10) Alexa Fluor® 594

sc-133155 AF594
200 µg/ml
RMB2685.00

eIF4G 抗体 (A-10) Alexa Fluor® 647

sc-133155 AF647
200 µg/ml
RMB2685.00

eIF4G 抗体 (A-10) Alexa Fluor® 680

sc-133155 AF680
200 µg/ml
RMB2685.00

eIF4G 抗体 (A-10) Alexa Fluor® 790

sc-133155 AF790
200 µg/ml
RMB2685.00

Which protein isoform does this recognize: EIF4G1, EIF4G2, EIF4G3 or all and in case it recognizes all, which bands correspond to what?

Asked by: abhishekb
Thank you for your question. This monoclonal antibody is raised against amino acids 1-300 of eIF4G1 of human origin (accession number Q04637). The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2019-06-16

Can eIF4G (A-10): sc-133155 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jenniferc
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, eIF4G (A-10): sc-133155 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from GoodGood results when used in western blot of human cells.
Date published: 2021-05-31
Rated 4 out of 5 by from Good bands without backgroundGood bands at 1:500 dilution, with different isoforms appearing
Date published: 2020-07-03
Rated 4 out of 5 by from Good signal but intense backgroundUsed it in 3% non-fat milk and got the expected band, but had intense background too.
Date published: 2020-05-10
Rated 5 out of 5 by from Works goodI got a sample antibody from santa cruz and it works well
Date published: 2019-08-19
Rated 5 out of 5 by from 功能很出色抗体很好用，很满意，希望继续合作。很好用，很好用，很好用，很好用，很好用，很好用，很好用，很好用，很好用，
Date published: 2019-06-13
Rated 5 out of 5 by from at least a (commercial) anti-4G that workswe used this antibody at 1/1000 in western blot BY FLUORESCENCE (near IF fluorescence ssecondary antibody dy-LIGHT) to detect eIF4G in monkey epithelial cells directely or after co-immunoprecipitation and it works beautifully.
Date published: 2018-06-08
Rated 1 out of 5 by from No signal for IHC on mouse mammary tumorsNo signal was detect on parafilm sections of mouse mammary tumors.
Date published: 2018-04-30
Rated 5 out of 5 by from works wellIt works well in western blot analysis with clean background.
Date published: 2017-10-15
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eIF4G 抗体 (A-10) is rated 4.5 out of 5 by 14.
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