Date published: 2025-9-8
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eIF3η Antibody (C-5) - Western Blotting - Image 359365
eIF3η 抗体 (C-5): sc-137214. Jurkat (A) 和 HeLa (B) 全细胞裂解液中 eIF3η 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
eIF3η 抗体 (C-5): sc-137214. Jurkat (A) 和 HeLa (B) 全细胞裂解液中 eIF3η 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

eIF3η 抗体 (C-5): sc-137214

4.1(8)
寫評論提問

说明书
  • eIF3 eta 抗体 C-5 是小鼠单克隆 IgG1 κ，eIF3η抗体, 在24篇文献中引用，规格为200 µg/ml
  • 针对氨基酸131-300的图谱，在eIF3η的内部区域，human起源
  • eIF3 eta 抗体 (C-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 eIF3η
  • 抗eIF3 eta抗体（C-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取eIF3η (C-5): sc-137214的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 eIF3η 抗体 (C-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与eIF3η 抗体 (C-5) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加eIF抗体，包括eIF1, eIF1AY, eIF2 α, eIF2B α, eIF2B β, eIF2B δ, eIF2B ε, eIF2 β, eIF2B γ, eIF2C, eIF2C2, eIF2C4, eIF2D, eIF3 p110, eIF3 α和eIF3 β

eIF3η抗体（C-5）是一种IgG1 κ小鼠单克隆eIF3 eta抗体（也称为EIF3-ETA抗体、真核翻译起始因子3亚基9 eta 116kDa抗体、EIF3-P116抗体、真核翻译起始因子3亚基B抗体或真核起始因子3（eIF3）蛋白复合物组分抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人的eIF3 eta蛋白。eIF3η抗体（C-5）既可以是非偶联的抗eIF3 eta抗体形式，也可以是多种偶联形式的抗eIF3 eta抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。真核细胞中蛋白质合成的起始受蛋白质起始因子和RNA分子之间相互作用的调节。真核起始因子（eIFs）参与一系列反应，最终促成80S核糖体的组装和翻译的进行。真核起始因子-3（eIF3）作为支架结构，是eIF复合物中最大的一个，包含eIF3α、eIF3β、eIF3γ、eIF3δ、eIF3ε、eIF3Ω、eIF3η和eIF3θ等多个亚基，共同控制40S核糖体亚基的组装。eIF3蛋白与40S核糖体亚基的结合能够稳定eIF2-GTP-Met-tRNAiMet复合物的结合以及mRNA的结合，并促进80S核糖体解离为40S和60S亚基，进而促进翻译前起始复合物的组装。多种癌症中常见eIF3蛋白的过度表达，提示eIF3蛋白在肿瘤发生中可能扮演重要角色。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

eIF3η 抗体 (C-5) 参考文献:

  1. 真核生物翻译起始因子 3（eIF3）亚基 NIP1/c 与 eIF1 和 eIF5 的相互作用促进了起始前复合体的组装并调节起始密码子的选择。  |  Valásek, L., et al. 2004. Mol Cell Biol. 24: 9437-55. PMID: 15485912
  2. eIF3：S6K1 与翻译前启动复合体的连接因子。  |  Peterson, TR. and Sabatini, DM. 2005. Mol Cell. 20: 655-7. PMID: 16337588
  3. 翻译起始因子 eIF4G-1 通过 eIF3e 亚基与 eIF3 结合。  |  LeFebvre, AK., et al. 2006. J Biol Chem. 281: 22917-32. PMID: 16766523
  4. 启动因子 eIF3 与 mRNA 翻译, 细胞生长和癌症的调控。  |  Dong, Z. and Zhang, JT. 2006. Crit Rev Oncol Hematol. 59: 169-80. PMID: 16829125
  5. eIF3：翻译起始复合物的多功能支架。  |  Hinnebusch, AG. 2006. Trends Biochem Sci. 31: 553-62. PMID: 16920360
  6. 人类翻译起始因子 eIF3 的五个亚基的单独过表达会引发不死成纤维细胞的恶性转化。  |  Zhang, L., et al. 2007. J Biol Chem. 282: 5790-800. PMID: 17170115
  7. 重组揭示了哺乳动物 eIF3 的功能核心。  |  Masutani, M., et al. 2007. EMBO J. 26: 3373-83. PMID: 17581632
  8. 麻疹病毒 N 蛋白通过与 eIF3-p40 结合抑制宿主翻译。  |  Sato, H., et al. 2007. J Virol. 81: 11569-76. PMID: 17686838
  9. 人类翻译起始因子 eIF3 的磷酸化 h 亚基的致癌作用。  |  Zhang, L., et al. 2008. J Biol Chem. 283: 24047-60. PMID: 18544531
  10. eIF3：影响人类健康和疾病的因素。  |  Gomes-Duarte, A., et al. 2018. RNA Biol. 15: 26-34. PMID: 29099306
  11. eIF3 与硒蛋白 mRNA 相互作用  |  Hayek, H., et al. 2022. Biomolecules. 12: PMID: 36139107
  12. eIF3 mRNA 选择性分析揭示了 eIF3k 是与癌症相关的核糖体含量调节因子。  |  Duan, H., et al. 2023. EMBO J. 42: e112362. PMID: 37155573

订购信息

产品名称产品编号规格价格数量收藏夹

eIF3η 抗体 (C-5)

sc-137214
200 µg/ml
RMB2377.00

eIF3η (C-5): m-IgG Fc BP-HRP 套装

sc-528876
200 µg Ab; 10 µg BP
RMB2662.00

eIF3η (C-5): m-IgGκ BP-HRP 套装

sc-521452
200 µg Ab, 40 µg BP
RMB2662.00

eIF3η (C-5): m-IgG1 BP-HRP 套装

sc-543185
200 µg Ab; 20 µg BP
RMB2662.00

eIF3η 抗体 (C-5) AC

sc-137214 AC
500 µg/ml, 25% agarose
RMB3129.00

eIF3η 抗体 (C-5) HRP

sc-137214 HRP
200 µg/ml
RMB2377.00

eIF3η 抗体 (C-5) FITC

sc-137214 FITC
200 µg/ml
RMB2482.00

eIF3η 抗体 (C-5) PE

sc-137214 PE
200 µg/ml
RMB2580.00

eIF3η 抗体 (C-5) Alexa Fluor® 488

sc-137214 AF488
200 µg/ml
RMB2685.00

eIF3η 抗体 (C-5) Alexa Fluor® 546

sc-137214 AF546
200 µg/ml
RMB2685.00

eIF3η 抗体 (C-5) Alexa Fluor® 594

sc-137214 AF594
200 µg/ml
RMB2685.00

eIF3η 抗体 (C-5) Alexa Fluor® 647

sc-137214 AF647
200 µg/ml
RMB2685.00

eIF3η 抗体 (C-5) Alexa Fluor® 680

sc-137214 AF680
200 µg/ml
RMB2685.00

eIF3η 抗体 (C-5) Alexa Fluor® 790

sc-137214 AF790
200 µg/ml
RMB2685.00

Can this antibody be used as bait to recover the full eIF3 complex out of total lysate of human cells?

Asked by: Ls87
Thank you for your question. This antibody has not yet been tested or published for this type of research application, so we do not know if it is can be used for this purpose.
Answered by: Technical Support
Date published: 2019-11-22

Can eIF3η (C-5): sc-137214 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Randy McDonald
Thank you for your question. Yes, eIF3η (C-5): sc-137214 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 3 out of 5 by from This antibody works great for human cell linesI tried this sample antibody for ICC in human HEK293T cells and it works great. However, this antibody does not have a specific staining for IF staining in mouse brain.
Date published: 2018-01-02
Rated 4 out of 5 by from eIF3η Antibody (C-5) for WB and IFIt provides consistently good results for WB and IF using Hek 293 and U2OS cell lines for endogenous protein detection. For IF, it is excellent (dilution 1:500).
Date published: 2017-10-24
Rated 5 out of 5 by from Great!This antibody produced strong and specific signals on WB without background.
Date published: 2017-05-31
Rated 3 out of 5 by from eIF3n (C-5) antibodyI got a sample of this antibody to compare with discontinued eIF3n (N-20). Although it is not as good as N-20, it can be used for western-blot and IP as shown in the figure.
Date published: 2017-03-07
Rated 5 out of 5 by from Robust signal obtained at correct molecularRobust signal obtained at correct molecular weight observed by Western Blot using A-431 and Jurkat nuclear extracts. -SCBT QC
Date published: 2015-05-02
Rated 5 out of 5 by from Publishable data obtained by immunofluorescencePublishable data obtained by immunofluorescence using mouse eIF3 transfected HeLa cells. -SCBT Publication Review
Date published: 2015-04-22
Rated 4 out of 5 by from Cytoplasmic localization of the eIF3 proteinCytoplasmic localization of the eIF3 protein demonstrated by immunofluorescence using methanol fixed HeLa cells. -SCBT QC
Date published: 2015-02-05
Rated 4 out of 5 by from Strong bands of expected molecular weightStrong bands of expected molecular weight observed by Western Blot using non-transfected and mouse eIF3 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-11-29
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eIF3η 抗体 (C-5) is rated 4.1 out of 5 by 8.
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