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eIF2C2 Antibody (4F9) - Western Blotting - Image 14964
用 eIF2C (4F9): sc-53521 免疫沉淀并用 eIF2C (H-300): sc-32877 检测 K-562 (A) 和 HeLa (B) 全细胞裂解液中 eIF2C 表达的 Western 印迹分析.
eIF2C2 Antibody (4F9) - Western Blotting - Image 14964
用 eIF2C (4F9): sc-53521 免疫沉淀并用 eIF2C (H-300): sc-32877 检测 K-562 (A) 和 HeLa (B) 全细胞裂解液中 eIF2C 表达的 Western 印迹分析.
用 eIF2C (4F9): sc-53521 免疫沉淀并用 eIF2C (H-300): sc-32877 检测 K-562 (A) 和 HeLa (B) 全细胞裂解液中 eIF2C 表达的 Western 印迹分析.

eIF2C2 抗体 (4F9): sc-53521

3.9(11)
寫評論提問

说明书
  • eIF2C2 抗体 4F9 是小鼠单克隆 IgA κ，eIF2C2抗体, 在20篇文献中引用，规格为200 µg/ml
  • 针对全长重组eIF2C2，human起源
  • 推荐用于 mouse, rat 和 human 来源的eIF2C2 WB, IP, IF 和 ELISA检测
  • 关于如何获取eIF2C2 (4F9): sc-53521的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成eIF2C2 抗体 (4F9)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 eIF 抗体 包括 eIF1, eIF1AY, eIF2 α, eIF2B α, eIF2B β, eIF2B δ, eIF2B ε, eIF2 β, eIF2B γ和eIF2C

eIF2C2抗体（4F9）是一种IgA κ小鼠单克隆eIF2C2抗体（也称为EIF2C2抗体、AGO2抗体或Argonaute 2抗体），可通过免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测来自小鼠、大鼠和人类的eIF2C2蛋白。eIF2C2抗体（4F9）为非偶联的抗eIF2C2抗体形式。真核翻译起始因子2C（eIF2C）蛋白（Argonaute家族）作为RNA诱导沉默复合体（RISC）或含有microRNA（miRNA）的核糖核蛋白颗粒（miRNP）的组成部分，影响RNA干扰（RNAi）。小RNA，包括小干扰RNA（siRNA）和miRNA，可以通过利用RISC或miRNP颗粒的机制来沉默靶基因。eIF2C1（Argonaute 1，AGO1，eIF2C，GERP95，Q99）和Dicer1在siRNA介导的基因沉默中起协同作用。eIF2C2（Slicer，Argonaute 2，AGO2，Q10）是RISC的一个组成部分，可以集中在细胞质处理体（P-bodies）中并催化mRNA裂解。哺乳动物P-bodies含有mRNA，并与miRNA诱导的翻译沉默和siRNA诱导的mRNA降解有关。其他eIF2C蛋白还包括eIF2C3（Argonaute 3，AGO3）、eIF2C4（Argonaute 4，AGO4）和meIF2c5（小鼠Argonaute 5）。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

eIF2C2 抗体 (4F9) 参考文献:

  1. 单链反义 siRNA 在 RNAi 中引导目标 RNA 的裂解。  |  Martinez, J., et al. 2002. Cell. 110: 563-74. PMID: 12230974
  2. Argonaute 家族：触角伸向 RNAi, 发育控制, 干细胞维持和肿瘤发生。  |  Carmell, MA., et al. 2002. Genes Dev. 16: 2733-42. PMID: 12414724
  3. PAZ 结构域的结构和保守的 RNA 结合。  |  Yan, KS., et al. 2003. Nature. 426: 468-74. PMID: 14615802
  4. 人类 Argonaute2 介导 miRNA 和 siRNA 靶向的 RNA 切割。  |  Meister, G., et al. 2004. Mol Cell. 15: 185-97. PMID: 15260970
  5. Argonaute2 是哺乳动物 RNAi 的催化引擎。  |  Liu, J., et al. 2004. Science. 305: 1437-41. PMID: 15284456
  6. 分子生物学Argonaute 进入 RISC 的核心。  |  Sontheimer, EJ. and Carthew, RW. 2004. Science. 305: 1409-10. PMID: 15353786
  7. Argonaute 2/RISC 位于哺乳动物 mRNA 的衰变位点，即细胞质体。  |  Sen, GL. and Blau, HM. 2005. Nat Cell Biol. 7: 633-6. PMID: 15908945
  8. 使用单克隆抗体检测 RNA 诱导沉默复合体 (RISC) 中的 argonaute 蛋白 Ago2 和 microRNA。  |  Ikeda, K., et al. 2006. J Immunol Methods. 317: 38-44. PMID: 17054975
  9. GW/P 体在 RNA 处理和沉默中的作用。  |  Jakymiw, A., et al. 2007. J Cell Sci. 120: 1317-23. PMID: 17401112
  10. 种系AGO2突变会损害RNA干扰和人类神经发育。  |  Lessel, D., et al. 2020. Nat Commun. 11: 5797. PMID: 33199684
  11. YTHDF1 通过 YTHDF1-AGO2 相互作用和相分离促进 mRNA 降解。  |  Li, J., et al. 2022. Cell Prolif. 55: e13157. PMID: 34821414
  12. AGO2 通过激活线粒体基因翻译来预防糖尿病性心肌病。  |  Zhan, J., et al. 2024. Circulation. 149: 1102-1120. PMID: 38126189

订购信息

产品名称产品编号规格价格数量收藏夹

eIF2C2 抗体 (4F9)

sc-53521
200 µg/ml
RMB2377.00

Hi. I'm writing you from Bronner's lab, CALTECH. We want to know if this antibody recognize Chick Ago2. Thanks

Asked by: Estefanía
Thank you for your question. Unfortunately we do not have any information regarding chicken reactivity, so we can not recommend it for that use.
Answered by: Technical Support
Date published: 2019-01-26

Is this antibody specific to Ago2 or does it crossreact with the other Argonaute proteins, namely Ago1, Ago3 or Ago4? Thank you for your help.

Asked by: francesca65
Thank you for your question. This monoclonal antibody, eIF2C2 (4F9): sc-53521, is specific for AGO2, or Argonaute 2, and does not cross-react with other Argonaute proteins.
Answered by: Technical Support
Date published: 2017-08-31

What is the recommended fixation method for IF with eIF2C2 (4F9): sc-53521 monoclonal antibody?

Asked by: jenni
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 13
Date published: 2017-01-20

What is the total volume provided of eIF2C2 (4F9): sc-53521?

Asked by: jenni
Each vial contains 200 µg IgA in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support 13
Date published: 2017-01-16
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Rated 2 out of 5 by from Poor band visibility in WBThis antibody was used at 1:500 dilution. The bands obtained were not very clear (in visibility or number) even at high exposition times. You can still assess the general protein quantity on WB but I recommend maybe to dilute it less.
Date published: 2024-03-14
Rated 1 out of 5 by from Unfortunately it does not workI got this antibody as a free sample and it doesnt work fine for western
Date published: 2022-09-02
Rated 2 out of 5 by from Not suitable for WBWe wanted to detect Ago2 in our sample via WB. So unfurtunatley for our purpose this AB did not work. Nevertheless it is also mentioned in the describtion to use another AB for detection.
Date published: 2018-04-10
Rated 5 out of 5 by from Strong westernVery clean western blot data; low background with strong signal
Date published: 2017-01-25
Rated 5 out of 5 by from Positive resultsPositive bands detected with eIF2C2 Antibody (4F9)
Date published: 2017-01-19
Rated 5 out of 5 by from Western Blot data was suitable for publicationWestern Blot data was suitable for publication using HeLa cells. -SCBT Publication Review
Date published: 2015-05-15
Rated 4 out of 5 by from Antibody scAntibody sc-53521 used successfully by Western Blot for human prostate cancer cells analysis. -SCBT Publication Review
Date published: 2015-03-12
Rated 5 out of 5 by from Good results obtained by Western Blot usingGood results obtained by Western Blot using human Huh7 cells. -SCBT Publication Review
Date published: 2015-03-02
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eIF2C2 抗体 (4F9) is rated 3.9 out of 5 by 11.
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