Date published: 2025-9-8
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eIF2Bγ Antibody (C-11) - Western Blotting - Image 366539
eIF2Bγ 抗体 (C-11): sc-137248. eIF2Bγ 在 HeLa 核提取物 (A), HL-60 (B) 和 MCF7 (C) 全细胞裂解液中的表达的 Western 印迹分析.
eIF2Bγ 抗体 (C-11): sc-137248. eIF2Bγ 在 HeLa 核提取物 (A), HL-60 (B) 和 MCF7 (C) 全细胞裂解液中的表达的 Western 印迹分析.

eIF2Bγ 抗体 (C-11): sc-137248

4.8(4)
寫評論提問

说明书
  • eIF2Bγ抗体(C-11)是小鼠单克隆IgG1 κ, 在4篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列153-452位于human物种的eIF2Bγ的C-terminus附近
  • 推荐用于 mouse, rat 和 human 来源的eIF2Bγ WB, IP, IF 和 ELISA检测
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是eIF2Bγ Antibody (C-11) for WB applications. 的首选辅助检测试剂，这些试剂现与eIF2Bγ Antibody (C-11) 打包提供（请参阅下面的订购信息）。

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基因信息
蛋白序列
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研究信息

関連項目

附加eIF抗体，包括eIF1, eIF1AY, eIF2 α, eIF2B α, eIF2B β, eIF2B δ, eIF2B ε, eIF2 β, eIF2B γ, eIF2C, eIF2C2, eIF2C4, eIF2D, eIF3 p110, eIF3 α和eIF3 β

蛋白质合成在真核细胞中的启动受蛋白质启动因子与RNA分子之间相互作用的调控。真核细胞启动复合物eIF2B由eIF2Bα、eIF2Bβ、eIF2Bγ、eIF2Bδ和eIF2Bε五个亚基组成。eIF2B复合物催化eIF2复合物上GDP与GTP的交换，这一过程是在eIF2/GTP与40S核糖体亚基的相互作用之后发生的。eIF2Bε亚基单独具有环腺苷核糖交换因子（GEF）活性，但在所有五个eIF2B亚基存在时其活性更强。eIF2的磷酸化抑制eIF2B的GEF活性，这种抑制需要eIF2Bα亚基。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

eIF2Bγ 抗体 (C-11)

sc-137248
200 µg/ml
RMB2377.00

eIF2Bγ (C-11): m-IgG Fc BP-HRP 套装

sc-539969
200 µg Ab; 10 µg BP
RMB2662.00

eIF2Bγ (C-11): m-IgG1 BP-HRP 套装

sc-541822
200 µg Ab; 20 µg BP
RMB2662.00

What is the recommended fixation method for immunofluorescence staining with eIF2Bγ (C-11): sc-137248 monoclonal antibody?

Asked by: Randy McDonald
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_9, d_7, h_4CST
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Rated 5 out of 5 by from Use it for Western Blot (1:1000) detectingUse it for Western Blot (1:1000) detecting total protein lysates (20 micro-gram) from mouse embryonic fibroblast cell line. Very specific (one band only) and very strong. Size is accurate, slightly below 55 Kd marker. I am very pleased with this antibody.
Date published: 2015-10-19
Rated 4 out of 5 by from Cytoplasmic localization of the eIF2B proteinCytoplasmic localization of the eIF2B protein observed when methanol fixed NIH/3T3 cells were analyzed by immunofluorescence. -SCBT QC
Date published: 2015-04-15
Rated 5 out of 5 by from Robust band observed for the human eIF2BRobust band observed for the human eIF2B protein when analyzed by Western Blot using HeLa nuclear extract. -SCBT QC
Date published: 2015-03-05
Rated 5 out of 5 by from Strong signal obtained at expected molecularStrong signal obtained at expected molecular weight for the human eIF2B protein by Western Blot using K-562 nuclear extract. -SCBT QC
Date published: 2014-09-29
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eIF2Bγ 抗体 (C-11) is rated 4.8 out of 5 by 4.
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