Date published: 2025-9-6

021-6093-6350

SCBT Portrait Logo
Seach Input

eIF2Bε 抗体 (B-7): sc-55558

4.8(5)
寫評論提問

说明书
  • eIF2B epsilon 抗体 B-7 是小鼠单克隆 IgG1 κ,eIF2Bε抗体, 在7篇文献中引用,规格为200 µg/ml
  • 免疫human来源eIF2Bε近C-terminus的422-711氨基酸
  • eIF2B epsilon 抗体 (B-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 eIF2Bε
  • 抗eIF2B epsilon抗体(B-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取eIF2Bε (B-7): sc-55558免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 eIF2Bε 抗体 (B-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与eIF2Bε 抗体 (B-7) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

関連項目

eIF2Bε 抗体 (B-7) 是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF)、免疫组织化学和酶联免疫吸附试验 (ELISA) 检测小鼠、大鼠和人源的 eIF2B epsilon 蛋白。eIF2Bε 抗体 (B-7) 有非共轭型和多种共轭型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。eIF2B epsilon 蛋白在调节真核细胞蛋白质合成启动过程中起着至关重要的作用,它是一种鸟嘌呤核苷酸交换因子,可促进 eIF2 复合物上 GDP 与 GTP 的交换。eIF2B 复合物由五个亚基组成:eIF2Bα、eIF2Bβ、eIF2Bγ、eIF2Bδ和 eIF2Bε,其中 eIF2Bε 表现出的 GEF 活性在整个复合物存在时显著增强。值得注意的是,eIF2 磷酸化可抑制 eIF2B 的 GEF 活性,而这一过程依赖于 eIF2Bα 亚基,这突显了控制蛋白质合成的复杂调控机制以及对细胞应激反应的潜在影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

eIF2Bε 抗体 (B-7) 参考文献:

  1. 编码大鼠翻译引发剂-2B α-亚基的基因的结构和序列。  |  Flowers, KM., et al. 1995. Biochim Biophys Acta. 1264: 163-7. PMID: 7495858
  2. 小鼠鸟嘌呤核苷酸交换因子 eIF-2B 的 delta 亚基。cDNA 的特征预测了氨基末端不同的异构体。  |  Henderson, RA., et al. 1994. J Biol Chem. 269: 30517-23. PMID: 7982969
  3. 兔和人的真核生物引发剂-2Bε亚基编码 cDNA 的克隆和特性鉴定。  |  Asuru, AI., et al. 1996. Biochim Biophys Acta. 1307: 309-17. PMID: 8688466
  4. 克隆哺乳动物翻译起始因子 2B(真核生物起始因子 2 的鸟嘌呤核苷酸交换因子)γ-亚基的 cDNA。  |  Price, NT., et al. 1996. Biochem J. 318 (Pt 2): 631-6. PMID: 8809057
  5. eIF2B,真核生物启动因子 2 的鸟嘌呤核苷酸交换因子。哺乳动物和酵母中 eIF2B 的 alpha, beta 和 delta 亚基之间的序列保持一致。  |  Price, NT., et al. 1996. Biochem J. 318 (Pt 2): 637-43. PMID: 8929216
  6. 在 Sf9 细胞中表达的真核生物引发剂-2B 的亚基组装和鸟嘌呤核苷酸交换活性。  |  Fabian, JR., et al. 1997. J Biol Chem. 272: 12359-65. PMID: 9139680
  7. 真核启动因子 2B(eIF2B)。  |  Webb, BL. and Proud, CG. 1997. Int J Biochem Cell Biol. 29: 1127-31. PMID: 9438375

订购信息

产品名称产品编号规格价格数量收藏夹

eIF2Bε 抗体 (B-7)

sc-55558
200 µg/ml
RMB2377.00

eIF2Bε (B-7): m-IgG Fc BP-HRP 套装

sc-526864
200 µg Ab; 10 µg BP
RMB2662.00

eIF2Bε (B-7): m-IgG1 BP-HRP 套装

sc-532237
200 µg Ab; 20 µg BP
RMB2662.00

eIF2Bε 抗体 (B-7) AC

sc-55558 AC
500 µg/ml, 25% agarose
RMB3129.00

eIF2Bε 抗体 (B-7) HRP

sc-55558 HRP
200 µg/ml
RMB2377.00

eIF2Bε 抗体 (B-7) FITC

sc-55558 FITC
200 µg/ml
RMB2482.00

eIF2Bε 抗体 (B-7) PE

sc-55558 PE
200 µg/ml
RMB2580.00

eIF2Bε 抗体 (B-7) Alexa Fluor® 488

sc-55558 AF488
200 µg/ml
RMB2685.00

eIF2Bε 抗体 (B-7) Alexa Fluor® 546

sc-55558 AF546
200 µg/ml
RMB2685.00

eIF2Bε 抗体 (B-7) Alexa Fluor® 594

sc-55558 AF594
200 µg/ml
RMB2685.00

eIF2Bε 抗体 (B-7) Alexa Fluor® 647

sc-55558 AF647
200 µg/ml
RMB2685.00

eIF2Bε 抗体 (B-7) Alexa Fluor® 680

sc-55558 AF680
200 µg/ml
RMB2685.00

eIF2Bε 抗体 (B-7) Alexa Fluor® 790

sc-55558 AF790
200 µg/ml
RMB2685.00

What starting dilution do you recommend for IF and Western?

Asked by: erhl
Thank you for your question. We recommend a starting dilution of 1:50 for IF and 1:200 for Western blotting. The recommended starting dilution(s) and a range are listed on our product data sheet. https://datasheets.scbt.com/sc-55558.pdf
Answered by: Technical Support
Date published: 2017-10-02

I am using eIF2Bε (B-7): sc-55558 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_8, d_30, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_55558, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 107ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Very Versatile AntibodyThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-02-07
Rated 5 out of 5 by from This antibody was successfully used withThis antibody was successfully used with rhesus monkey samples via Western Blot. -SCBT Publication Review
Date published: 2015-03-05
Rated 4 out of 5 by from Cytoplasmic staining of glandular cells observedCytoplasmic staining of glandular cells observed by immunoperoxidase staining of formalin fixed, paraffin embedded (FFPE) human stomach tissue. -SCBT QC
Date published: 2015-02-20
Rated 5 out of 5 by from Specificity demonstrated by Western BlotSpecificity demonstrated by Western Blot, comparing 293T cells that have been transfected to over-express the human eIF2B protein with 293T cells that have not been transfected. An increase in signal observed in cells that have been transfected. -SCBT QC
Date published: 2015-01-06
Rated 5 out of 5 by from Robust bands at the expected molecular weightRobust bands at the expected molecular weight observed by Western Blot using HeLa, NIH/3T3 and KNRK whole cell lysates. -SCBT QC
Date published: 2013-01-24
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_5
  • loc_zh_CN, sid_55558, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 10ms
  • REVIEWS, PRODUCT
eIF2Bε 抗体 (B-7) is rated 4.8 out of 5 by 5.
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_5
  • loc_zh_CN, sid_55558, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 119ms
  • REVIEWS, PRODUCT