Date published: 2025-9-7
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eIF2A Antibody (3A7A8) - Western Blotting - Image 358312
eIF2A 抗体 (3A7A8): sc-517214. 人重组 eIF2A (448-576) 融合蛋白的 Western 印迹分析.
eIF2A 抗体 (3A7A8): sc-517214. 人重组 eIF2A (448-576) 融合蛋白的 Western 印迹分析.

eIF2A 抗体 (3A7A8): sc-517214

3.3(6)
寫評論提問

说明书
  • eIF2A 抗体 3A7A8 是小鼠单克隆 IgG1，eIF2A抗体, 在6篇文献中引用，规格为50 µg/0.5 ml
  • 与重组蛋白结合，该重组蛋白与eIF2A的448-576的氨基酸对应，来源于human
  • 推荐用于 mouse, rat 和 human 来源的eIF2A WB, IP, IF, IHC(P), FCM 和 ELISA检测
  • 关于如何获取eIF2A (3A7A8): sc-517214的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成eIF2A 抗体 (3A7A8)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

此外 eIF 抗体包括 eIF1, eIF1AY, eIF2 α, eIF2B α, eIF2B β, eIF2B δ, eIF2B ε, eIF2 β, eIF2B γ 和 eIF2C

eIF2A抗体（3A7A8）是一种小鼠单克隆IgG1抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）、流式细胞术（FCM）和酶联免疫吸附 酶联免疫吸附试验（ELISA）。eIF2A (3A7A8) 抗体有非结合型可供选择。eIF2A 蛋白，也称为 CDA02、MSTP004 或 MSTP089，是一种由 585 个氨基酸组成的蛋白，具有三个 WD 重复序列，对翻译起始过程至关重要 。eIF2A蛋白的表达非常普遍，在心脏、大脑、胰腺和胎盘中的含量最高，这表明其在细胞代谢和蛋白质合成中起着至关重要的作用。eIF2A通过与蛋氨酰-tRNA结合并将其引导至40S核糖体亚基来发挥翻译起始因子功能，这是蛋白质合成早期阶段的关键步骤。与以GTP依赖方式运作的eIF2复合物不同，eIF2A以密码子依赖方式促进这一过程，突显其独特的调节能力。此外，eIF2A可能在特定蛋白的负调控中发挥作用，表明其可能具有转录抑制剂的功能。由于可进行选择性剪接，eIF2A存在两种同工型，这有助于其在不同细胞环境中发挥不同的功能。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

eIF2A 抗体 (3A7A8) 参考文献:

  1. 哺乳动物 eIF2A 的特征和酵母同源物的鉴定。  |  Zoll, WL., et al. 2002. J Biol Chem. 277: 37079-87. PMID: 12133843
  2. 酵母真核细胞引发剂 2A 生物特性的新特点。  |  Komar, AA., et al. 2005. J Biol Chem. 280: 15601-11. PMID: 15718232
  3. 纤连蛋白通过β1整合素和真核生物引发因子4和2协调途径控制帽子依赖性翻译。  |  Gorrini, C., et al. 2005. Proc Natl Acad Sci U S A. 102: 9200-5. PMID: 15961545
  4. 真核生物蛋白质合成的机制和调节。  |  Merrick, WC. 1992. Microbiol Rev. 56: 291-315. PMID: 1620067
  5. 双链 RNA 依赖性蛋白激酶磷酸化真核翻译引发剂 2 的α-亚基介导细胞凋亡。  |  Scheuner, D., et al. 2006. J Biol Chem. 281: 21458-21468. PMID: 16717090
  6. 抑制核糖体募集可诱导应激颗粒形成，与真核启动因子 2α 磷酸化无关。  |  Mazroui, R., et al. 2006. Mol Biol Cell. 17: 4212-9. PMID: 16870703
  7. eIF2 的磷酸化引导 ATF5 在不同应激条件下的翻译控制。  |  Zhou, D., et al. 2008. J Biol Chem. 283: 7064-73. PMID: 18195013
  8. HepG2 人类肝癌细胞受到氨基酸限制后，eIF2alpha 的磷酸化需要 MEK 信号。  |  Thiaville, MM., et al. 2008. J Biol Chem. 283: 10848-57. PMID: 18287093

订购信息

产品名称产品编号规格价格数量收藏夹

eIF2A 抗体 (3A7A8)

sc-517214
50 µg/0.5 ml
RMB2377.00

Do you have any IF images you can share?

Asked by: esprngl
Thank you for your question. Unfortunately we do not have any published IF images at this time. We do recommend and warranty this antibody for IF use. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-06-27

What is the recommended storage condition for eIF2A (3A7A8): sc-517214?

Asked by: Professor Griffin
Thank you for your question. We recommend storing the antibody at 4° C.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by from Good for Western BlotThis antibody detects bands at the expected molecular weight in MCF-7, PC-12, HepG2, HeLa, and Cos7 whole cell lysates. -SCBT QC
Date published: 2023-09-14
Rated 1 out of 5 by from Did not work for meThe product did not work for my epithelial cell lysate sample at 1. 1:1000 concentration 2. Incubation overnight at room temperature 3. High secondary concentration
Date published: 2018-10-09
Rated 5 out of 5 by from GreatThis eIF2A antibody is great for western in MDA-MB-231 cells
Date published: 2018-02-02
Rated 1 out of 5 by from eIF2a sc-517214 does not work!The sample eIF2a (3A7A8) sc-517214 does not work and strong unspecific band
Date published: 2017-11-04
Rated 4 out of 5 by from Good antibodyImmunoprecipitation followed by Western Blotting showed good enrichment in the eIF2A IP compared to low amounts of cell lysate (eIF2A and IgG Input controls). The eIF2A-detected band showed up at the expected size of ~60 kDa and indicates two distinct eIF2A isoforms in the Input controls.
Date published: 2017-10-11
Rated 4 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
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eIF2A 抗体 (3A7A8) is rated 3.3 out of 5 by 6.
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