Date published: 2025-9-6

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EGFR 抗体 (R-1): sc-101

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说明书
  • EGFR抗体(R-1)是小鼠单克隆IgG2b κ, 在114篇文献中引用,规格为200 µg/ml
  • 针对human表皮样癌细胞系A431的,在human的氨基酸6-273和EGFR之间进行表位定位
  • 抗-EGFR 抗体 (R-1) 推荐用于 WB, IP, IF 和 FCM,检测mouse, rat 和human 来源的 EGFR
  • 抗EGFR抗体(R-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还提供无叠氮化物,用于生物学研究,sc-101 L,200 µg/0.1 ml
  • 关于如何获取EGFR (R-1): sc-101免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP是EGFR Antibody (R-1) 适用于 WB 应用。 的首选辅助检测试剂,这些试剂现与EGFR Antibody (R-1) 打包提供(请参阅下面的订购信息)。

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関連項目

EGFR抗体(R-1)是一种针对人类表皮生长因子受体(EGFR)的小鼠单克隆IgG2b kappa轻链抗体,EGFR是一种参与细胞信号传导和生长调节的关键受体酪氨酸激酶。EGFR小鼠单克隆抗体(R-1)可识别EGFR免疫原序列中的表位,并表现出广泛的物种交叉反应性,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和流式细胞术(FCM)检测小鼠、大鼠和人类样本中的EGFR。抗EGFR单克隆抗体(R-1)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和各种Alexa Fluor®结合物,可满足各种实验需求。EGFR在细胞表面充当复杂的信号转导器,对包括表皮生长因子(EGF)和转化生长因子α(TGFα)在内的特定配体作出反应。配体结合后,EGFR发生二聚化并激活内源性酪氨酸激酶活性,触发下游信号级联,如RAS-RAF-MAPK和PI3K-AKT通路。这些途径调节细胞的基本过程,包括增殖、存活、分化,并在器官形态发生和组织修复中发挥重要作用。当EGFR信号传递失调时,特别是通过过度表达或突变,异常激活会促进肿瘤发生,导致细胞无控制地生长、侵袭和转移。由于EGFR在许多癌细胞中普遍表达,因此它是一个重要的治疗靶点,抑制EGFR功能可以诱导癌细胞周期停滞和凋亡。EGFR (R-1)抗体为研究人员提供了研究这些复杂信号传导机制和开发靶向癌症疗法的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

EGFR 抗体 (R-1)

sc-101
200 µg/ml
RMB2377.00

EGFR (R-1): m-IgG Fc BP-HRP 套装

sc-528096
200 µg Ab; 10 µg BP
RMB2662.00

EGFR (R-1): m-IgGκ BP-HRP 套装

sc-520394
200 µg Ab, 40 µg BP
RMB2662.00

EGFR 抗体 (R-1) L

sc-101 L
200 µg/0.1 ml
RMB2377.00

EGFR 抗体 (R-1) AC

sc-101 AC
500 µg/ml, 25% agarose
RMB3129.00

EGFR 抗体 (R-1) HRP

sc-101 HRP
200 µg/ml
RMB2377.00

EGFR 抗体 (R-1) FITC

sc-101 FITC
200 µg/ml
RMB2482.00

EGFR 抗体 (R-1) PE

sc-101 PE
200 µg/ml
RMB2580.00

EGFR 抗体 (R-1) Alexa Fluor® 488

sc-101 AF488
200 µg/ml
RMB2685.00

EGFR 抗体 (R-1) Alexa Fluor® 546

sc-101 AF546
200 µg/ml
RMB2685.00

EGFR 抗体 (R-1) Alexa Fluor® 594

sc-101 AF594
200 µg/ml
RMB2685.00

EGFR 抗体 (R-1) Alexa Fluor® 647

sc-101 AF647
200 µg/ml
RMB2685.00

EGFR 抗体 (R-1) Alexa Fluor® 680

sc-101 AF680
200 µg/ml
RMB2685.00

EGFR 抗体 (R-1) Alexa Fluor® 790

sc-101 AF790
200 µg/ml
RMB2685.00

Can I get a free sample of an antibody (sc-365829) to try for my application?  Can I try several of your EGF-R antibodies to determine the optimal reagent for my use (sc-120 & sc-101)? Thank You

Asked by: MAntczak
We currently offer sample sizes for all 3 of these EGFR antibodies. Please contact our Technical Service Department for current information on how to receive a free 10 ug monoclonal antibody sample.
Answered by: Technical Support
Date published: 2020-04-09

bonjour,  savez-vous si la reconnaissance de la protéine par l'anticorps est dépendante des N-glycosylations de l'EGFR ?

Asked by: Serge
merci pour votre question. Veuillez contacter notre Service Technique par email [email protected] pour plus d'informations sur ce produit.
Answered by: Tech Support Europe
Date published: 2019-12-16

How can EGFR Antibody (R-1): sc-101 be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. EGFR Antibody (R-1): sc-101 is currently available conjugated to phycoerythrin (sc-101 PE), fluoroscein (sc-101 FITC), Alexa Fluor® 488 (sc-101 AF488), Alexa Fluor® 647 (sc-101 AF647), PerCP (sc-101 PerCP) or Alexa Fluor® 405 (sc-101 AF405)
Answered by: Technical Support 2
Date published: 2017-01-09
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Rated 5 out of 5 by from Bright and specificThis antibody works very well for proximity ligation assay in human oral epithelial cell line.
Date published: 2023-10-04
Rated 1 out of 5 by from Not detectableWe had no detection of bands with this antibody when testing.
Date published: 2021-11-22
Rated 5 out of 5 by from Great featuresI bought this a month ago and I'm so happy that I did
Date published: 2020-11-19
Rated 4 out of 5 by from Worked wellGave nice strong bands for WB at 1:200. Used with mouse samples.
Date published: 2018-03-22
Rated 5 out of 5 by from A good choice for EGFR detectionThis antibody is a great choice. The IF data is features strong and specific signals while Western blots will result in bright clean bands. Researchers have published with this antibody over 30 times.
Date published: 2017-01-17
Rated 5 out of 5 by from Outstanding!!Great IF and western blot results;far superior to polyclonal alternatives
Date published: 2017-01-06
Rated 5 out of 5 by from no commentno comment
Date published: 2015-05-15
Rated 5 out of 5 by from Worked well for Western blot with HUVEC samplesWorked well for Western blot with HUVEC samples. -SCBT Publication Review
Date published: 2015-03-27
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EGFR 抗体 (R-1) is rated 4.6 out of 5 by 12.
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