Date published: 2025-9-6

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EGFR 抗体 (C-2): sc-377229

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说明书
  • EGFR抗体(C-2)是小鼠单克隆IgG2a κ, 在10篇文献中引用,规格为200 µg/ml
  • 特定于human起源的EGFR的C-terminal细胞质域内氨基酸1131-1169之间的表位图谱
  • 抗-EGFR 抗体 (C-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 EGFR
  • 抗EGFR抗体(C-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取EGFR (C-2): sc-377229免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG2a BP-HRP 是 EGFR 抗体 (C-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与EGFR 抗体 (C-2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

EGFR抗体(C-2)是一种IgG2a κ小鼠单克隆EGFR抗体,可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的EGFR。EGFR抗体(C-2)既可以作为非偶联的抗EGFR抗体形式,也可以作为多种偶联形式的抗EGFR抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。表皮生长因子受体家族包括几种相关的受体酪氨酸激酶,它们在多种癌症中经常被过度表达。该受体家族的成员包括EGFR(HER1)、Neu(ErbB-2,HER2)、ErbB-3(HER3)和ErbB-4(HER4),它们在配体结合后形成同二聚体或异二聚体。EGFR基因产物中的外显子经常被删除或复制,分别产生缺失突变体(DM)或串联重复突变体(TDM),这些突变体以不同的分子量检测到。EGFR结合多种配体,包括表皮生长因子(EGF)、转化生长因子α(TGFα)、双调蛋白和肝素结合-EGF(HB-EGF)。配体结合通过网格蛋白包被的凹坑促进EGFR的内化,并随后响应其内在酪氨酸激酶而降解。EGFR参与器官形态发生和组织的维持和修复,但EGFR的上调与肿瘤进展有关。EGFR的致癌作用包括启动DNA合成、增强细胞生长、侵袭和转移。EGFR的缺失导致癌症细胞周期阻滞、凋亡或去分化,提示EGFR可能是一种有效的治疗靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

EGFR 抗体 (C-2)

sc-377229
200 µg/ml
RMB2377.00

EGFR (C-2): m-IgG Fc BP-HRP 套装

sc-526091
200 µg Ab; 10 µg BP
RMB2662.00

EGFR (C-2): m-IgG2a BP-HRP 套装

sc-546501
200 µg Ab; 10 µg BP
RMB2662.00

EGFR 抗体 (C-2) AC

sc-377229 AC
500 µg/ml, 25% agarose
RMB3129.00

EGFR 抗体 (C-2) HRP

sc-377229 HRP
200 µg/ml
RMB2377.00

EGFR 抗体 (C-2) FITC

sc-377229 FITC
200 µg/ml
RMB2482.00

EGFR 抗体 (C-2) PE

sc-377229 PE
200 µg/ml
RMB2580.00

EGFR 抗体 (C-2) Alexa Fluor® 488

sc-377229 AF488
200 µg/ml
RMB2685.00

EGFR 抗体 (C-2) Alexa Fluor® 546

sc-377229 AF546
200 µg/ml
RMB2685.00

EGFR 抗体 (C-2) Alexa Fluor® 594

sc-377229 AF594
200 µg/ml
RMB2685.00

EGFR 抗体 (C-2) Alexa Fluor® 647

sc-377229 AF647
200 µg/ml
RMB2685.00

EGFR 抗体 (C-2) Alexa Fluor® 680

sc-377229 AF680
200 µg/ml
RMB2685.00

EGFR 抗体 (C-2) Alexa Fluor® 790

sc-377229 AF790
200 µg/ml
RMB2685.00

EGFR (C-2) 中和勝肽

sc-377229 P
100 µg/0.5 ml
RMB511.00

In mouse tissue (Mus musculus) what would be an appropriate positive control to use?

Asked by: RTMac
Thank you for your question. A good positive control for this antibody would be Hep G2 Cell Lysate : sc-2227 https://www.scbt.com/p/hep-g2-cell-lysate?requestFrom=search -Tech Service
Answered by: Tech Service 1
Date published: 2021-02-04

What is the predicted size of this protein for western blot?

Asked by: JDM4
Thank you for your question. The molecular weight is predicted to be 170 kDa.
Answered by: Technical Support
Date published: 2019-01-06

I need to use an antibody to detect the protein in samples of non-mammalian origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 15
Date published: 2017-01-11

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-11
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Rated 4 out of 5 by from Works fantastic!This antibody is very good for IF. 1:50 is a very good concentration to use
Date published: 2019-09-25
Rated 5 out of 5 by from Nice strong band!Yielded a strong band when used at 1:200 probing mouse and human lysates via WB.
Date published: 2018-03-23
Rated 5 out of 5 by from Superb WB and IHCGreat product for western blot applications; also outstanding IHC
Date published: 2017-01-29
Rated 5 out of 5 by from Great antibody!The EGFR Antibody (C-2): sc-377229 performs very well for IHC applications when using formalin fixed, paraffin-embedded tissue sections. This antibody has also been cited several times for further verification.
Date published: 2017-01-13
Rated 5 out of 5 by from Good EGFR control antibody for CRISPR/Cas9Good EGFR control antibody for CRISPR/Cas9 KO experiment -SCBT QC
Date published: 2015-04-10
Rated 4 out of 5 by from Blowout band in EGF treated ABlowout band in EGF treated A-431 and good signal with BT-20 whole cell lysates. -SCBT QC
Date published: 2015-02-09
Rated 5 out of 5 by from Great antibody for paraffin embedded sectionsGreat antibody for paraffin embedded sections. The sc-377229, EGFR antibody gives a strong membrane and cytoplasmic signal in human vagina tissue. -SCBT QC
Date published: 2014-05-11
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EGFR 抗体 (C-2) is rated 4.7 out of 5 by 7.
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