Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
EGFR Antibody (A-10) - Western Blotting - Image 392271
表皮生长因子受体抗体 (A-10) Alexa Fluor® 680: sc-373746 AF680. BT-20 (A), A-431 (B) 和 MDA-MB-231 (C) 全细胞裂解液中表皮生长因子受体表达的直接近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
表皮生长因子受体抗体 (A-10) Alexa Fluor® 680: sc-373746 AF680. BT-20 (A), A-431 (B) 和 MDA-MB-231 (C) 全细胞裂解液中表皮生长因子受体表达的直接近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

EGFR 抗体 (A-10): sc-373746

4.6(65)
寫評論提問

说明书
  • EGFR 抗体 A-10 是小鼠单克隆 IgG2a κ，EGFR抗体, 在238篇文献中引用，规格为200 µg/ml
  • 特定于human起源的EGFR的C-terminal细胞质域内的1114-1147之间的表位图谱
  • EGFR 抗体 (A-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 EGFR
  • 抗EGFR抗体（A-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取EGFR (A-10): sc-373746的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 EGFR 抗体 (A-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与EGFR 抗体 (A-10) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

EGFR抗体（A-10）是一种IgG2aκ小鼠单克隆EGFR抗体（也称为HER1抗体或ERBB1抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类来源的EGFR蛋白。EGFR抗体（A-10）既可以作为非偶联的抗EGFR抗体形式，也可以作为多种偶联形式的抗EGFR抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。表皮生长因子受体家族包括几种相关的受体酪氨酸激酶，它们在多种癌症中经常被过度表达。该受体家族的成员包括EGFR（HER1）、Neu（ErbB-2，HER2）、ErbB-3（HER3）和ErbB-4（HER4），它们在配体结合后形成同二聚体或异二聚体。EGFR基因产物中的外显子经常被删除或复制，分别产生缺失突变体（DM）或串联重复突变体（TDM），这些突变体以不同的分子量检测到。EGFR结合多种配体，包括表皮生长因子（EGF）、转化生长因子α（TGFα）、双调蛋白和肝素结合-EGF（HB-EGF）。配体结合通过网格蛋白包被的凹坑促进EGFR的内化，并随后响应其内在酪氨酸激酶而降解。EGFR参与器官形态发生和组织的维持和修复，但EGFR的上调与肿瘤进展有关。EGFR的致癌作用包括启动DNA合成、增强细胞生长、侵袭和转移。EGFR的缺失导致癌症细胞周期阻滞、凋亡或去分化，提示EGFR可能是一种有效的治疗靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

EGFR 抗体 (A-10)

sc-373746
200 µg/ml
RMB2377.00

EGFR (A-10): m-IgG Fc BP-HRP 套装

sc-529548
200 µg Ab; 10 µg BP
RMB2662.00

EGFR (A-10): m-IgGκ BP-HRP 套装

sc-522480
200 µg Ab, 40 µg BP
RMB2662.00

EGFR (A-10): m-IgG2a BP-HRP 套装

sc-547328
200 µg Ab; 10 µg BP
RMB2662.00

EGFR 抗体 (A-10) AC

sc-373746 AC
500 µg/ml, 25% agarose
RMB3129.00

EGFR 抗体 (A-10) HRP

sc-373746 HRP
200 µg/ml
RMB2377.00

EGFR 抗体 (A-10) FITC

sc-373746 FITC
200 µg/ml
RMB2482.00

EGFR 抗体 (A-10) PE

sc-373746 PE
200 µg/ml
RMB2580.00

EGFR 抗体 (A-10) Alexa Fluor® 488

sc-373746 AF488
200 µg/ml
RMB2685.00

EGFR 抗体 (A-10) Alexa Fluor® 546

sc-373746 AF546
200 µg/ml
RMB2685.00

EGFR 抗体 (A-10) Alexa Fluor® 594

sc-373746 AF594
200 µg/ml
RMB2685.00

EGFR 抗体 (A-10) Alexa Fluor® 647

sc-373746 AF647
200 µg/ml
RMB2685.00

EGFR 抗体 (A-10) Alexa Fluor® 680

sc-373746 AF680
200 µg/ml
RMB2685.00

EGFR 抗体 (A-10) Alexa Fluor® 790

sc-373746 AF790
200 µg/ml
RMB2685.00

EGFR (A-10) 中和勝肽

sc-373746 P
100 µg/0.5 ml
RMB511.00

Hello, I have EGFR(A-10): sc373746 antibody and p-EGFR (9H2): sc-57545, I would like to ask dilution for both and secondary reagent please . 

Asked by: 143KB
Thank you for your question. For Western Blot we recommend a starting dilution 1:100. The dilution range is 1:100-1:1000. More recommendations are given in the datasheet, which can be downloaded from the product page.
Answered by: Tech Support Europe
Date published: 2024-01-16

EGFR(A-10): sc-373746  Is the amino acid number of the epitope 1114-1147 counted from first Methionine or after cleavage of the signal peptide?

Asked by: Sakuchan
Thank you for your question. EGFR Antibody (A-10): sc-373746 is specific for an epitope mapping between amino acids 1114-1147 within a C-terminal cytoplasmic domain of EGFR of human origin. If you have a further question, please contact the local distributor or technical service.
Answered by: Sen Li
Date published: 2024-01-16

Can EGFR antibody A-10 sc-373746 be used on slide-fixed tumor cells (not on paraffin biopsies)?

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2024-01-16

Does this ab recognize the mouse EGFR in tissus (IF), what is the dilution recommended? Thanks

Asked by: Any21
Thank you for your question. Yes, this antibody is recommended for IF/IHC on mouse tissue samples. We recommend a starting dilution of 1:50 for this antibody.
Answered by: Tech Service
Date published: 2024-04-17

Does this antibody against mutant forms of EGFR in mouse or human?

Asked by: Po Lee
Thank you for your question. EGFR Antibody (A-10): sc-373746 is specific for an epitope mapping between amino acids 1114-1147 within a C-terminal cytoplasmic domain of EGFR of human origin. EGFR proteins possessing this amino acid sequence should be recognized by this antibody without issue.
Answered by: Tech Support Europe
Date published: 2024-01-16

if I probe western blot with EGFR sc-373746, I don't have to against the blot with 2nd antibody. Is that right? Is this a phospho-EGFR antibody?

Asked by: Daisy II
This antibody is available unconjugated (sc-373476), in which case you must use a secondary anti-mouse IgG for detection. It is also available directly conjugated to HRP, and fluorescent probes, (sc-373476 HRP, etc.), in which case there is no secondary antibody needed for detection. This is not a phospho-specific EGFR antibody. It is reactive with total EGFR.
Answered by: Technical Support
Date published: 2024-04-17

HI, I need to do negative depletion of cells using an anti-EGF-receptor monoclonal antibody, mouse-human. can I use EGFR Antibody (A-10): sc-373746 for this purpose?

Asked by: Maria Vernon
Thank you for your question. We have not tested this antibody for any biological activity such as depletion, and so this is not a recommended application at this time. We do offer an alternative antibody which has been validated for use in biological studies, including neutralization, EGFR Antibody (R-1): sc-101, also provided azide-free for biological studies, sc-101 L.
Answered by: Technical Support
Date published: 2024-06-30

Is it positive for MCF-7 or MDA-MB-231 ?

Asked by: Gracess
Thank you for your question. We have published Western blot data on our website using this antibody in MDA-MB-231 whole cell lysates. We do not have any data in MCF-7.
Answered by: Technical Support
Date published: 2024-01-16
  • y_2025, m_8, d_31, h_8CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_11
  • loc_zh_CN, sid_373746, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 122ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Anti-EGFFR antibody worked well in IHCAnti-EGFFR antibody worked well in IHC using human tissues
Date published: 2025-06-06
Rated 3 out of 5 by from Exelente resultadoComprei este produti mes passado e adorei produto facil de usar
Date published: 2024-02-13
Rated 5 out of 5 by from FuncionalidadeProduto faz o que promete, eficiente e responsabilidade pelo produto
Date published: 2024-02-09
Rated 5 out of 5 by from ÚsalaEs la mejor app úsala cambio mi vida por completo te la recomiendo y le doy 5 estrellas
Date published: 2023-10-10
Rated 5 out of 5 by from EGFR (A-10): sc-373746The specificity of the antibody is high, and the bands are clearly visible
Date published: 2023-05-15
Rated 5 out of 5 by from BonoHo prenso questo e stra Bono recomanda a titti amici
Date published: 2023-01-22
Rated 5 out of 5 by from very good antibodyI can detect band! The antibody was used for Western Blot in hepa1c1c7 cells, it works at a concentration of 1: 100 ~ 200.
Date published: 2022-12-07
Rated 4 out of 5 by from DetectableThis is a good antibody, we were able to detect in testing.
Date published: 2021-11-22
  • y_2025, m_8, d_31, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_65
  • loc_zh_CN, sid_373746, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT
EGFR 抗体 (A-10) is rated 4.6 out of 5 by 66.
  • y_2025, m_8, d_31, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_65
  • loc_zh_CN, sid_373746, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 104ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。