Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
EGF Antibody (F-9) - Western Blotting - Image 109572
EGF 抗体 (F-9): sc-166779. 人血小板提取物中 EGF 表达的 Western 印迹分析.
EGF Antibody (F-9) - Western Blotting - Image 109572
EGF 抗体 (F-9): sc-166779. 人血小板提取物中 EGF 表达的 Western 印迹分析.
EGF 抗体 (F-9): sc-166779. 人血小板提取物中 EGF 表达的 Western 印迹分析.

EGF 抗体 (F-9): sc-166779

4.3(6)
寫評論提問

说明书
  • EGF 抗体 F-9 是小鼠单克隆 IgG1 κ，EGF抗体, 在6篇文献中引用，规格为200 µg/ml
  • 针对氨基酸971-1023的抗原，代表成熟EGF，来源于human
  • EGF 抗体 (F-9) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 precursor and mature EGF
  • 抗EGF抗体（F-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取EGF (F-9): sc-166779的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是EGF Antibody (F-9) for WB applications. 的首选辅助检测试剂，这些试剂现与EGF Antibody (F-9) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

EGF抗体（F-9）是一种IgG1 κ小鼠单克隆EGF抗体（也称为受体酪氨酸激酶（RTK）表皮生长因子受体（EGFR）配体EGF抗体、53个氨基酸的EGF抗体或尿抑胃素抗体），可通过WB、IP、IF和ELISA检测人源性的EGF蛋白。EGF抗体（F-9）既可以是非偶联的抗EGF抗体形式，也可以是多种偶联形式的抗EGF抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。表皮生长因子（EGF）是一种耐酸和耐热的53个氨基酸的蛋白质，最初在啮齿动物和人类中发现。体内和体外实验均表明，它对多种细胞类型具有强效的促有丝分裂作用。EGF与细胞表面的EGF受体结合，并介导受体在酪氨酸残基上的内在磷酸化。已在几乎所有的体液中检测到它，如尿液（尿抑胃素）、唾液、牛奶和富血小板血浆。EGF、TGFα和牛痘病毒生长因子具有30-40%的氨基酸同源性。已描述了EGF/TGF家族的几个额外成员，这些包括Cripto、Amphiregulin和肝素结合的EGF样生长因子。Amphiregulin和肝素结合的EGF样生长因子都与EGF受体结合。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

EGF 抗体 (F-9) 参考文献:

  1. 异源机制激活EGF受体。  |  Carraway, KL. and Sweeney, C. 2002. Cancer Cell. 1: 405-6. PMID: 12124166
  2. 分离小鼠颌下腺蛋白质，加速新生动物门牙萌出和眼睑张开。  |  COHEN, S. 1962. J Biol Chem. 237: 1555-62. PMID: 13880319
  3. 表皮生长因子相关蛋白在人类结直肠肿瘤中的差异表达。  |  Ciardiello, F., et al. 1991. Proc Natl Acad Sci U S A. 88: 7792-6. PMID: 1715580
  4. 疫苗病毒生长因子的纯化和特征。  |  Stroobant, P., et al. 1985. Cell. 42: 383-93. PMID: 2410141
  5. EGF受体转运：对信号传导和癌症的影响。  |  Tomas, A., et al. 2014. Trends Cell Biol. 24: 26-34. PMID: 24295852
  6. 压力揭示了EGF受体的新目的地。  |  Tomas, A. and Futter, CE. 2015. Cell Cycle. 14: 3343-4. PMID: 26418166
  7. 表皮生长因子, 其受体和相关蛋白。  |  Carpenter, G. and Zendegui, JG. 1986. Exp Cell Res. 164: 1-10. PMID: 3007185
  8. 表皮生长因子和其他多肽有丝分裂原的受体。  |  Carpenter, G. 1987. Annu Rev Biochem. 56: 881-914. PMID: 3039909
  9. 转化生长因子-α：结构和生物活性。  |  Derynck, R. 1986. J Cell Biochem. 32: 293-304. PMID: 3543030
  10. 胚胎植入过程中HB-EGF与VANGL2信号传导的意外讨论。  |  Kim, YS., et al. 2023. Proc Natl Acad Sci U S A. 120: e2302937120. PMID: 37155852
  11. 尿囊素-表皮生长因子的体内方面。  |  Gregory, H. 1985. J Cell Sci Suppl. 3: 11-7. PMID: 3914989
  12. 人类表皮生长因子（尿囊素）的放射免疫测定。  |  Starkey, RH. and Orth, DN. 1977. J Clin Endocrinol Metab. 45: 1144-53. PMID: 304067

订购信息

产品名称产品编号规格价格数量收藏夹

EGF 抗体 (F-9)

sc-166779
200 µg/ml
RMB2377.00

EGF (F-9): m-IgG Fc BP-HRP 套装

sc-527187
200 µg Ab; 10 µg BP
RMB2662.00

EGF (F-9): m-IgG1 BP-HRP 套装

sc-532560
200 µg Ab; 20 µg BP
RMB2662.00

EGF 抗体 (F-9) AC

sc-166779 AC
500 µg/ml, 25% agarose
RMB3129.00

EGF 抗体 (F-9) HRP

sc-166779 HRP
200 µg/ml
RMB2377.00

EGF 抗体 (F-9) FITC

sc-166779 FITC
200 µg/ml
RMB2482.00

EGF 抗体 (F-9) PE

sc-166779 PE
200 µg/ml
RMB2580.00

EGF 抗体 (F-9) Alexa Fluor® 488

sc-166779 AF488
200 µg/ml
RMB2685.00

EGF 抗体 (F-9) Alexa Fluor® 546

sc-166779 AF546
200 µg/ml
RMB2685.00

EGF 抗体 (F-9) Alexa Fluor® 594

sc-166779 AF594
200 µg/ml
RMB2685.00

EGF 抗体 (F-9) Alexa Fluor® 647

sc-166779 AF647
200 µg/ml
RMB2685.00

EGF 抗体 (F-9) Alexa Fluor® 680

sc-166779 AF680
200 µg/ml
RMB2685.00

EGF 抗体 (F-9) Alexa Fluor® 790

sc-166779 AF790
200 µg/ml
RMB2685.00

sobre el anticuerpo anti-EGF, solo quisiera saber si cruza con rata

Asked by: Anonymous
Gracias por su pregunta. El anticuerpo no debería reaccionar de forma cruzada con rata y este anticuerpo se recomienda para muestras humanas.
Answered by: BlakeJ
Date published: 2025-01-16

Can EGF (F-9): sc-166779 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, EGF (F-9): sc-166779 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_8, d_30, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_166779, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 103ms
  • QUESTIONS, PRODUCT
Rated 2 out of 5 by from questionable qualityI tried this antibody, but I had to run it at an extremely high concentration in the trial format in order to see any kind of band.
Date published: 2018-01-22
Rated 4 out of 5 by from great for WBA nice antibody for Weston Blot.I chose dilution of 1:300,and I got a good result.
Date published: 2017-07-11
Rated 5 out of 5 by from Antibody is really great.Antibody is really great,can be used in many cell lines.
Date published: 2017-04-28
Rated 5 out of 5 by from Produced publishable IF dataThis EGF monoclonal antibody was referenced in the literature for the use of IF on HiCHO cells.
Date published: 2017-01-23
Rated 5 out of 5 by from Excellent resultExcellent Western blot data of human recombinant EGF protein, with specific band and even no background.
Date published: 2016-11-22
Rated 5 out of 5 by from Good Western blot data of EGF expressionGood Western blot data of EGF expression in human platelet extract. -SCBT QC
Date published: 2014-07-13
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_zh_CN, sid_166779, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT
EGF 抗体 (F-9) is rated 4.3 out of 5 by 6.
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_zh_CN, sid_166779, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 105ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。