Date published: 2025-9-6

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EG-VEGF 抗体 (E-12): sc-390741

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说明书
  • EG-VEGF 抗体 E-12 是小鼠单克隆 IgG2b κ,EG-VEGF抗体, 在2篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列66-105位于human物种的EG-VEGF的C-terminus
  • EG-VEGF 抗体 (E-12) 推荐用于WB, IP, IF 和 ELISA,检测mouse, rat, human 来源的 precursor and mature EG-VEGF; 可能与 Prokineticin-2产生交联反应
  • 抗EG-VEGF抗体(E-12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取EG-VEGF (E-12): sc-390741免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRPm-IgGκ BP-HRP是EG-VEGF Antibody (E-12) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与EG-VEGF Antibody (E-12) 打包提供(请参阅下面的订购信息)。

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関連項目

EG-VEGF抗体(E-12)是一种IgG2b κ小鼠单克隆EG-VEGF抗体(也称为PK1抗体、PROK1抗体或前动力蛋白1抗体),它通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的EG-VEGF蛋白。EG-VEGF抗体(E-12)既有非偶联形式的抗EG-VEGF抗体,也有多种偶联形式的抗EG-VEGF抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。内分泌腺衍生的血管内皮生长因子(EG-VEGF)可诱导来自内分泌腺的毛细血管内皮细胞的增殖、迁移和开窗形成。EG-VEGF具有HIF-1结合位点;其表达由缺氧诱导,并局限于类固醇生成腺(卵巢、睾丸、肾上腺和胎盘)。EG-VEGF的表达通常与VEGF的表达互补,这表明这些分子以协调的方式发挥作用。EG-VEGF是一类高度特异性的有丝分裂原的一个例子,它们以组织特异性的方式调节血管内皮的增殖和分化。它主要在一种类型的组织中表达,并选择性地作用于一种类型的内皮细胞。EG-VEGF可能通过与G蛋白偶联受体结合,导致MAPK p44/42和磷脂酰肌醇3-激酶信号通路的激活,从而导致响应的内皮细胞的增殖、迁移和存活。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

EG-VEGF 抗体 (E-12) 参考文献:

  1. 心血管生物学。创造独特的血管  |  Carmeliet, P. 2001. Nature. 412: 868-9. PMID: 11528460
  2. 鉴定一种对内分泌腺内皮细胞具有选择性的血管生成有丝分裂原。  |  LeCouter, J., et al. 2001. Nature. 412: 877-84. PMID: 11528470
  3. 肾上腺皮质毛细血管内皮细胞中源自内分泌腺的血管内皮生长因子信号的特征。  |  Lin, R., et al. 2002. J Biol Chem. 277: 8724-9. PMID: 11751915
  4. 由促肾上腺素/内分泌腺血管内皮生长因子激活的两种密切相关的 G 蛋白偶联受体的鉴定和分子特征。  |  Lin, DC., et al. 2002. J Biol Chem. 277: 19276-80. PMID: 11886876
  5. 分离和鉴定 EG-VEGF/prokineticins 作为两种孤儿 G 蛋白偶联受体的同源配体。  |  Masuda, Y., et al. 2002. Biochem Biophys Res Commun. 293: 396-402. PMID: 12054613
  6. 内分泌腺血管内皮生长因子(EG-VEGF)与血管生成的组织特异性调节假说。  |  Ferrara, N., et al. 2002. Endocr Res. 28: 763-4. PMID: 12530695
  7. EG-VEGF 和 Bv8:组织限制性血管生成因子新家族。  |  Ferrara, N., et al. 2004. Biochim Biophys Acta. 1654: 69-78. PMID: 14984768
  8. EG-VEGF:内分泌特异性血管生成, 内皮表型和功能的新型介质。  |  Lecouter, J., et al. 2004. Ann N Y Acad Sci. 1014: 50-7. PMID: 15153419
  9. EG-VEGF:胎盘发育过程中的关键内分泌因子。  |  Brouillet, S., et al. 2012. Trends Endocrinol Metab. 23: 501-8. PMID: 22709436
  10. EG-VEGF 沉默可通过 PI3K/AKT/mTOR 信号通路抑制胰腺癌细胞增殖并促进细胞凋亡。  |  Yan, X., et al. 2019. Biomed Pharmacother. 109: 762-769. PMID: 30551529
  11. 垂体腺瘤中 VEGF、VEGF165b 和 EG-VEGF 的表达与激素谱有特殊关系。  |  Corlan, AS., et al. 2019. Eur J Histochem. 63: PMID: 30838843
  12. EG-VEGF 通过 PROKR2 诱导人类滋养层细胞系的侵袭  |  Tani, K., et al. 2021. Acta Med Okayama. 75: 677-684. PMID: 34955534

订购信息

产品名称产品编号规格价格数量收藏夹

EG-VEGF 抗体 (E-12)

sc-390741
200 µg/ml
RMB2377.00

EG-VEGF (E-12): m-IgGκ BP-HRP 套装

sc-523661
200 µg Ab, 40 µg BP
RMB2662.00

EG-VEGF (E-12): m-IgG2b BP-HRP 套装

sc-549131
200 µg Ab; 10 µg BP
RMB2662.00

EG-VEGF 抗体 (E-12) AC

sc-390741 AC
500 µg/ml, 25% agarose
RMB3129.00

EG-VEGF 抗体 (E-12) HRP

sc-390741 HRP
200 µg/ml
RMB2377.00

EG-VEGF 抗体 (E-12) FITC

sc-390741 FITC
200 µg/ml
RMB2482.00

EG-VEGF 抗体 (E-12) PE

sc-390741 PE
200 µg/ml
RMB2580.00

EG-VEGF 抗体 (E-12) Alexa Fluor® 488

sc-390741 AF488
200 µg/ml
RMB2685.00

EG-VEGF 抗体 (E-12) Alexa Fluor® 546

sc-390741 AF546
200 µg/ml
RMB2685.00

EG-VEGF 抗体 (E-12) Alexa Fluor® 594

sc-390741 AF594
200 µg/ml
RMB2685.00

EG-VEGF 抗体 (E-12) Alexa Fluor® 647

sc-390741 AF647
200 µg/ml
RMB2685.00

EG-VEGF 抗体 (E-12) Alexa Fluor® 680

sc-390741 AF680
200 µg/ml
RMB2685.00

EG-VEGF 抗体 (E-12) Alexa Fluor® 790

sc-390741 AF790
200 µg/ml
RMB2685.00

What is the recommended starting dilution for Western blot for sc-390741: EG-VEGF (E-12) monoclonal antibody?

Asked by: AbPolly
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Great featuresAlthough there is no trend but the strip is obvious
Date published: 2017-09-05
Rated 5 out of 5 by from Good signalGood signal obtained in WB using human brain tissue lysastes.I highly recommend .
Date published: 2017-02-26
Rated 5 out of 5 by from golden antibodyI use EG-VEGF antibody by Immunohistochemistry of human tonsil tissue stained with Anti-E-Selectin antibody , We got specific staining. It's your ideal choice to do drugtest.
Date published: 2017-02-12
Rated 5 out of 5 by from Strong signal and clear bands.An accidental opportunity I used this antibody, the antibody WB in the 1:500 person with strong signals and clear bands.
Date published: 2017-02-08
Rated 5 out of 5 by from good resultgood results, the antibody works well for western blot.We use 1/500 dilution get specific bands.
Date published: 2017-02-03
Rated 5 out of 5 by from protein was majorly detectedAlthough reducing condition was applied, the observed size suggested a dimerized form of protein was majorly detected.
Date published: 2017-01-29
Rated 5 out of 5 by from WBI have not detected prok1 signal by this antibody in human macrophage samples, maybe the expression level is very low.
Date published: 2017-01-19
Rated 5 out of 5 by from The antibody is of good sensitivity.The product is of good sensitivity and specific degree. I brought it four month ago. The result is well by weston blot.
Date published: 2016-12-23
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EG-VEGF 抗体 (E-12) is rated 5.0 out of 5 by 9.
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