Date published: 2025-9-7

021-6093-6350

SCBT Portrait Logo
Seach Input

EF-1 α1 抗体 (CBP-KK1): sc-21758

4.5(13)
寫評論提問

说明书
  • EF-1 alpha 1 抗体 CBP-KK1 是小鼠单克隆 IgG1 κ,EF-1 α1抗体, 在27篇文献中引用,规格为200 µg/ml
  • 针对布鲁氏锥虫钙离子结合蛋白
  • EF-1 alpha 1 抗体 (CBP-KK1) 推荐用于WB, IP, IF 和 IHC(P),检测mammalian 来源的 EF-1 α1
  • 抗EF-1 alpha 1抗体(CBP-KK1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取EF-1 α1 (CBP-KK1): sc-21758免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 EF-1 α1 抗体 (CBP-KK1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与EF-1 α1 抗体 (CBP-KK1) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品,请参阅我们的小鼠 IgG 结合蛋白完整列表。

快捷链接

関連項目

EF-1 α1 抗体(CBP-KK1)是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和石蜡包埋切片免疫组织化学(IHCP)检测哺乳动物来源的 EF-1 α1 蛋白。抗EF-1 α1抗体(CBP-KK1)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。EF-1α1作为伸长因子-1复合物的组成部分,在蛋白质合成中起着至关重要的作用,该复合物在翻译过程中将氨基酰tRNA传递到核糖体中是必不可少的。这一过程确保了准确高效的蛋白质合成,这对于许多细胞功能和整体机体健康是必不可少的。EF-1α1在脑、胎盘、肺、肝、肾和胰腺等组织中表现出显著的表达,凸显了其广泛的生理重要性。EF-1 α1位于6q14.1染色体上,已被确定为费尔蒂综合征(Felty syndrome)的一种自身抗原,该综合征的特征是类风湿性关节炎和感染易感性增加。对于研究翻译机制和相关病理的研究人员来说,了解EF-1 α1的功能仍然至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

EF-1 α1 抗体 (CBP-KK1) 参考文献:

  1. 参与蛋白质生物合成的因子的可能进化。  |  Nyborg, J. 1998. Acta Biochim Pol. 45: 883-94. PMID: 10397336
  2. 通过修饰延伸因子 Tu 来调节翻译。  |  Kraal, B., et al. 1999. Folia Microbiol (Praha). 44: 131-41. PMID: 10588048
  3. 核糖体上翻译因子的 GTPases 机制和功能。  |  Rodnina, MV., et al. 2000. Biol Chem. 381: 377-87. PMID: 10937868
  4. 嗜热菌延伸因子 G 的结构域 III 对于诱导核糖体上的 GTP水解至关重要。  |  Martemyanov, KA. and Gudkov, AT. 2000. J Biol Chem. 275: 35820-4. PMID: 10940297
  5. 真核翻译延长因子1-α1抑制p53和p73依赖性细胞凋亡和化疗敏感性。  |  Blanch, A., et al. 2013. PLoS One. 8: e66436. PMID: 23799104
  6. 真核细胞延伸因子 1 alpha 1(EEF1A1)在禽再病毒 sigma-C 诱导的细胞凋亡和病毒生长抑制中的关键作用。  |  Zhang, Z., et al. 2015. Arch Virol. 160: 1449-61. PMID: 25854689
  7. 一种新型 3'tRNA 衍生片段 tRF-Val 通过靶向 EEF1A1 促进胃癌增殖并抑制凋亡。  |  Cui, H., et al. 2022. Cell Death Dis. 13: 471. PMID: 35585048
  8. 纳米粒子(NPs)介导的lncBCMA沉默,以促进eEF1A1泛素化并抑制乳腺癌的生长和转移。  |  Yang, K., et al. 2023. Acta Pharm Sin B. 13: 3489-3502. PMID: 37655325
  9. EEF1A1参与帕金森病神经炎症过程的调节。  |  Aisha, Z., et al. 2023. J Integr Neurosci. 22: 122. PMID: 37735116
  10. UCHL3通过去泛素化稳定EEF1A1,从而促进肝细胞癌的发展。  |  Zhao, J., et al. 2024. Biol Direct. 19: 53. PMID: 38965582
  11. N-乙酰半胱氨酸通过下调真核翻译延长因子1α1抑制柯萨奇病毒B3的复制。  |  Wang, Y., et al. 2024. Viruses. 16: PMID: 39339978
  12. 大肠杆菌 70S 核糖体上延伸因子 G 的可视化:转位机制。  |  Agrawal, RK., et al. 1998. Proc Natl Acad Sci U S A. 95: 6134-8. PMID: 9600930

订购信息

产品名称产品编号规格价格数量收藏夹

EF-1 α1 抗体 (CBP-KK1)

sc-21758
200 µg/ml
$316.00

EF-1 α1 (CBP-KK1): m-IgGκ BP-HRP 套装

sc-520730
200 µg Ab, 40 µg BP
$354.00

EF-1 α1 抗体 (CBP-KK1) AC

sc-21758 AC
500 µg/ml, 25% agarose
$416.00

EF-1 α1 抗体 (CBP-KK1) HRP

sc-21758 HRP
200 µg/ml
$316.00

EF-1 α1 抗体 (CBP-KK1) FITC

sc-21758 FITC
200 µg/ml
$330.00

EF-1 α1 抗体 (CBP-KK1) PE

sc-21758 PE
200 µg/ml
$343.00

EF-1 α1 抗体 (CBP-KK1) Alexa Fluor® 488

sc-21758 AF488
200 µg/ml
$357.00

EF-1 α1 抗体 (CBP-KK1) Alexa Fluor® 546

sc-21758 AF546
200 µg/ml
$357.00

EF-1 α1 抗体 (CBP-KK1) Alexa Fluor® 594

sc-21758 AF594
200 µg/ml
$357.00

EF-1 α1 抗体 (CBP-KK1) Alexa Fluor® 647

sc-21758 AF647
200 µg/ml
$357.00

EF-1 α1 抗体 (CBP-KK1) Alexa Fluor® 680

sc-21758 AF680
200 µg/ml
$357.00

EF-1 α1 抗体 (CBP-KK1) Alexa Fluor® 790

sc-21758 AF790
200 µg/ml
$357.00

Hello, I have used this antibody for western blot and I can see two bands, one located just below the 50 kDa marker and the other one just above 35. Can I just check if this antibody can give an aspecific band at 35kDa. Could it be degradation?

Asked by: Alessio81
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-08-10

Have you ever checked whether these antibodies recognize eEF1A2 isoform?

Asked by: elongator
Thank you for your question. We have not specifically checked the reactivity for eEF1A2, but this well characterized clone has been shown to be specific for eEF1A1.
Answered by: Tech Service
Date published: 2019-04-10

Will this antibody work in my porcine samples?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 4
Date published: 2017-01-13

I am planning to do some IF in my mouse samples. How can I do this with a mouse monoclonal antibody?

Asked by: Cweed
We recommend using directly conjugated antibodies. This product, sc-21758, is available conjugated to AF488, AF647, and FITC.
Answered by: Technical Support 4
Date published: 2017-01-13
  • y_2025, m_8, d_30, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_zh_CN, sid_21758, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 116ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from No hubo reconocimiento de la proteinaNo hubo formación de banda a la altura esperada en el marcador de proteínas en celulas C6/36 de mosquito
Date published: 2024-01-18
Rated 5 out of 5 by from WORKS WELL IN WBI got a clear band in 1:100 dilution, the band is bettwen 45kDa~60kDa
Date published: 2023-03-28
Rated 4 out of 5 by from Great antibodyThis antibody works very effectively in western blot. I can get a strong signal band in 293T cells.
Date published: 2018-04-10
Rated 5 out of 5 by from WELL FOR WBIT WORKED WELL BY WB IN LAB, WILL PURCHASE IT IN THE FUTURE
Date published: 2017-02-20
Rated 5 out of 5 by from IHC and western are greatOutstanding IHC staining results; also strong western data
Date published: 2017-01-23
Rated 5 out of 5 by from Good staining in IHCThis EF-1 alpha 1 antibody shows good results in mouse and human for IHC staining.
Date published: 2017-01-14
Rated 4 out of 5 by from We use this for western blottingWe use this for western blotting - no problems.
Date published: 2015-09-09
Rated 5 out of 5 by from Published data for WB in human H460 and H1299Published data for WB in human H460 and H1299 cells. -SCBT Publication Review
Date published: 2015-07-04
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_13
  • loc_zh_CN, sid_21758, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT
EF-1 α1 抗体 (CBP-KK1) is rated 4.5 out of 5 by 13.
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_13
  • loc_zh_CN, sid_21758, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 102ms
  • REVIEWS, PRODUCT