Date published: 2026-11-7

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EBV gp250/350 Envelope Protein 抗体 (0221): sc-57724

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说明书
  • EBV gp250/350 Envelope Protein 抗体 0221 是小鼠单克隆 IgG1，EBV gp250/350 Envelope Protein抗体, 在6篇文献中引用，规格为100 µg/ml
  • 针对EB病毒感染细胞
  • 推荐用于WB和IF检测Epstein-Barr Virus
  • m-IgG Fc BP-HRP是EBV gp250/350 Envelope Protein Antibody (0221) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与EBV gp250/350 Envelope Protein Antibody (0221) 搭配使用（请参阅下面的订购信息）。
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関連項目

附加EBV抗体，包括EBV Bam HI Z, EBV Bcl-2, EBV Ea-D, EBV EBNA-1, EBV gp110 Envelope Protein, EBV gp350 Envelope Protein, EBV LMP-1, EBV LMP-2A, EBV viral capsid antigen和EBV ZEBRA

EBV gp250/350包膜蛋白抗体（0221）是一种IgG1小鼠单克隆EBV gp250/350包膜蛋白抗体（也称为EBV gp250/350包膜蛋白抗体），可通过蛋白质印迹（WB）和免疫荧光（IF）检测Epstein-Barr病毒的EBV gp250/350包膜蛋白。EBV gp250/350包膜蛋白抗体（0221）可作为非偶联的抗EBV gp250/350包膜抗体使用。Epstein-Barr病毒（EBV），也被称为人类疱疹病毒4（HHV-4），是疱疹病毒家族的一员，也是最常见的人类病毒之一。EBV感染B细胞，尽管通常无症状，但它可引起传染性单核细胞增多症，这是一种以疲劳、发热、喉咙痛和肌肉酸痛为特征的疾病。EBV使用其主要包膜糖蛋白350（gp350）与人体B细胞上的细胞表面受体2（CR2）结合，因此，EBV gp350包膜蛋白，也称为EBV包膜糖蛋白复合物250/350，在介导EBV感染的初始阶段至关重要。EBV gp350包膜蛋白在病毒颗粒包膜以及EBV生产细胞上表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

含汞：所有HRP偶联物（包括组合装）均含有硫柳汞，这是一种汞化合物。请勿丢弃在垃圾中。应按照当地、州和联邦法规，将其作为危险化学品废弃物进行处置。

下载硫柳汞安全数据表（SDS）

EBV gp250/350 Envelope Protein 抗体 (0221) 参考文献:

  1. Epstein-Barr 病毒包膜糖蛋白 gp350 在 PKC 和 PI3-K 参与下诱导人单核细胞-巨噬细胞中 NF-kappaB 的活化和 IL-1beta 的合成。  |  D'Addario, M., et al. 1999. FASEB J. 13: 2203-13. PMID: 10593868
  2. Epstein-Barr 病毒主要包膜糖蛋白 gp350 与单核细胞结合后，通过涉及 PKC, PI3-K 和酪氨酸激酶的 NF-kappaB，导致 TNF-α 基因表达上调。  |  D'Addario, M., et al. 2000. J Mol Biol. 298: 765-78. PMID: 10801347
  3. 人类肺成纤维细胞感染 Epstein-Barr 病毒会导致 IL-1beta 和 bFGF 产物增加。  |  Adachi, H., et al. 2001. Exp Lung Res. 27: 157-71. PMID: 11258803
  4. Epstein-Barr 病毒糖蛋白 gp350 和 gp25 在人类上皮细胞感染中的作用。  |  Maruo, S., et al. 2001. J Gen Virol. 82: 2373-2383. PMID: 11562531
  5. 人类 CD21 晶体结构：对 Epstein-Barr 病毒和 C3d 结合的影响。  |  Prota, AE., et al. 2002. Proc Natl Acad Sci U S A. 99: 10641-6. PMID: 12122212
  6. 抗自然杀伤细胞活性的转染细胞上表达的 Epstein-Barr 病毒（EBV）糖蛋白 gp350 是 EBV 特异性抗体依赖性细胞毒性的靶抗原。  |  Khyatti, M., et al. 1991. J Virol. 65: 996-1001. PMID: 1846213
  7. 鉴定 Epstein-Barr 病毒主要包膜蛋白中介导病毒与 B 淋巴细胞 EBV 受体 (CR2) 结合的表位。  |  Nemerow, GR., et al. 1989. Cell. 56: 369-77. PMID: 2464439
  8. Vero细胞表达的爱泼斯坦-巴氏病毒（EBV）gp350/220能保护狨猴免受EBV挑战。  |  Emini, EA., et al. 1989. J Med Virol. 27: 120-3. PMID: 2537882

订购信息

产品名称产品编号规格价格数量收藏夹

EBV gp250/350 Envelope Protein 抗体 (0221)

sc-57724
100 µg/ml
CNY2422.00

EBV gp250/350 Envelope Protein (0221): m-IgG Fc BP-HRP 套装

sc-539067
100 µg Ab; 10 µg BP
CNY2715.00

Do you know what epitope of gp250/350 this antibody binds to? I am looking for something that binds to somewhere between aa2 and aa425. Thanks

Asked by: Becky
Thank you for your question. We have not performed epitope mapping for this antibody as of yet, so its exact binding site is not known at this point in time.
Answered by: Tech Support Europe
Date published: 2025-11-12

The delivered tube is named gp340/220 instead of gp250/350. Is this the same product? Does it still react with gp350? Please update your product sheet and website. It's confusing.

Asked by: Kathy S
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 option 2, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-08-24

What is the total volume provided of EBV gp340/gp220 Envelope Protein (0221): sc-57724 monoclonal antibody?

Asked by: Trav11
Thank you for your question. This antibody is provided as 100 µg in 1 ml total volume.
Answered by: Technical Support
Date published: 2018-07-04
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Rated 5 out of 5 by CVP6 from Works well for whole mount stainingUse at 1 ug/mL overnight in block. Staining is cytoplasmic. Cells fixed in 4% paraformaldehyde at room temp, permeabilized in 1% triton-X100 in PBS for 5 mins, washed in 0.05% Tween-20 in PBS 3x5mins, blocked in 1% BSA in PBS for 1 hr at room-temp.
Date published: 2025-12-12
Rated 4 out of 5 by Anonymous from Works well enoughWorks well enough by western (1:100) but not that strong
Date published: 2025-04-10
Rated 5 out of 5 by 小妍格格 from 功能很出色之前做了两个月，一直跑不出来这个蛋白，一个月前申请的试用装小样，效果非常好，现在来买正品，有santa家抗体保驾护航，希望实验越来越顺利，。
Date published: 2023-06-05
Rated 5 out of 5 by khys2 from EBV gp350 - immunofluorescenceGreat for immunofluorescence. Strong cytosolic staining of EBV gp350, use at 1:100, fixed in paraformaldehyde.
Date published: 2021-07-23
Rated 5 out of 5 by DefinitelyNotMatt from Reliable Ab for WBGreat monoclonal for use in WB, strong clear signal with no background.
Date published: 2017-01-25
Rated 4 out of 5 by JL from Publishable IF using human B cellsPublishable IF using human B cells - SCBT Publication Review
Date published: 2014-01-28
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EBV gp250/350 Envelope Protein抗体 (0221) is rated 4.7 out of 5 by 6.
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