Date published: 2025-9-7
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EBF Antibody (C-8) - Western Blotting - Image 56235
EBF 抗体 (C-8): sc-137065. 拉莫斯 (A) 和 IMR-32 (B) 全细胞裂解液中 EBF 表达的 Western 印迹分析.
EBF 抗体 (C-8): sc-137065. 拉莫斯 (A) 和 IMR-32 (B) 全细胞裂解液中 EBF 表达的 Western 印迹分析.

EBF 抗体 (C-8): sc-137065

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说明书
  • EBF 抗体 C-8 是小鼠单克隆 IgG2a κ，EBF抗体, 在14篇文献中引用，规格为200 µg/ml
  • 针对氨基酸1-300，在EBF的human起源的N-terminus处进行映射
  • EBF 抗体 (C-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 EBF1, EBF2, EBF3 and EBF4; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 抗EBF抗体（C-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取EBF (C-8): sc-137065的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 EBF 抗体 (C-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与EBF 抗体 (C-8) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 EBF 抗体包括 和 EBF3

EBF抗体（C-8）是一种IgG2a κ小鼠单克隆EBF抗体（也称为COE3抗体、EBF转录因子3抗体、早期B细胞因子3抗体、Olf-1/EBF样2抗体、EBF-3抗体、O/E-2抗体或OE-2抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源EBF蛋白。EBF抗体（C-8）既可以是非偶联抗EBF抗体形式，也可以是多种偶联形式的抗EBF抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。B淋巴细胞成熟是一个复杂的过程，需要一组独特的转录因子，这些转录因子与细胞分化和细胞谱系的阶段有关。在B细胞发育的早期阶段涉及的转录调节因子中，早期B细胞因子（EBF），也称为嗅觉神经元转录因子1（OLF1），靶向B淋巴样激酶（Blk）的启动子元件和编码早期阶段B细胞受体（BCR）部分的基因，这对于启动Ig轻链基因重组和Src激酶（Blk）信号传导是必需的。EBF是一种基本的螺旋-环-螺旋（bHLH）同源二聚体转录因子，由两个亚基组成，它们通过包含锌配位基序的DNA识别结构域与核心DNA序列CCCNNGGG相互作用。已经显示某些编码嗅觉相关蛋白的神经元特异性基因的启动子元件包含EBF结合位点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

EBF 抗体 (C-8) 参考文献:

  1. 早期 B 细胞因子是早期 B 细胞发育过程中 B 淋巴激酶启动子的激活剂。  |  Akerblad, P. and Sigvardsson, M. 1999. J Immunol. 163: 5453-61. PMID: 10553071
  2. 早期 B 细胞因子和碱性螺旋-环-螺旋蛋白的重叠表达是决定 lambda5 启动子的 B 系特异性活性的机制。  |  Sigvardsson, M. 2000. Mol Cell Biol. 20: 3640-54. PMID: 10779354
  3. 早期B细胞因子、E2A和Pax-5共同激活早期B细胞特异性mb-1启动子。  |  Sigvardsson, M., et al. 2002. Mol Cell Biol. 22: 8539-51. PMID: 12446773
  4. 早期 B 细胞因子对 p300/CBP 的抑制。  |  Zhao, F., et al. 2003. Mol Cell Biol. 23: 3837-46. PMID: 12748286
  5. 早期B细胞因子相关锌指基因是小鼠B细胞淋巴瘤中逆转录病毒整合的常见目标。  |  Warming, S., et al. 2004. Oncogene. 23: 2727-31. PMID: 15048087
  6. EBF表达减少是造血祖细胞B细胞潜能随年龄增长而丧失的根本原因。  |  Lescale, C., et al. 2010. Aging Cell. 9: 410-9. PMID: 20331442
  7. 缺乏转录因子 EBF 的小鼠 B 细胞分化失败。  |  Lin, H. and Grosschedl, R. 1995. Nature. 376: 263-7. PMID: 7542362
  8. EBF 包含一个新颖的锌配位基团以及多个二聚化和转录激活结构域。  |  Hagman, J., et al. 1995. EMBO J. 14: 2907-16. PMID: 7796816
  9. 通过酵母中的遗传选择，分子克隆嗅觉神经元转录因子 Olf-1。  |  Wang, MM. and Reed, RR. 1993. Nature. 364: 121-6. PMID: 8321284
  10. EBF 和 E47 协同诱导内源性免疫球蛋白替代轻链基因的表达。  |  Sigvardsson, M., et al. 1997. Immunity. 7: 25-36. PMID: 9252117
  11. 碱性螺旋-环-螺旋转录因子E12诱导早期B细胞因子（EBF）和多个B系基因。  |  Kee, BL. and Murre, C. 1998. J Exp Med. 188: 699-713. PMID: 9705952
  12. B29（免疫球蛋白 beta 链）基因是早期 B 细胞因子的遗传靶标。  |  Akerblad, P., et al. 1999. Mol Cell Biol. 19: 392-401. PMID: 9858563

订购信息

产品名称产品编号规格价格数量收藏夹

EBF 抗体 (C-8)

sc-137065
200 µg/ml
RMB2377.00

EBF (C-8): m-IgG Fc BP-HRP 套装

sc-528852
200 µg Ab; 10 µg BP
RMB2662.00

EBF (C-8): m-IgGκ BP-HRP 套装

sc-521412
200 µg Ab, 40 µg BP
RMB2662.00

EBF (C-8): m-IgG2a BP-HRP 套装

sc-547190
200 µg Ab; 10 µg BP
RMB2662.00

EBF 抗体 (C-8) AC

sc-137065 AC
500 µg/ml, 25% agarose
RMB3129.00

EBF 抗体 (C-8) HRP

sc-137065 HRP
200 µg/ml
RMB2377.00

EBF 抗体 (C-8) FITC

sc-137065 FITC
200 µg/ml
RMB2482.00

EBF 抗体 (C-8) PE

sc-137065 PE
200 µg/ml
RMB2580.00

EBF 抗体 (C-8) Alexa Fluor® 488

sc-137065 AF488
200 µg/ml
RMB2685.00

EBF 抗体 (C-8) Alexa Fluor® 546

sc-137065 AF546
200 µg/ml
RMB2685.00

EBF 抗体 (C-8) Alexa Fluor® 594

sc-137065 AF594
200 µg/ml
RMB2685.00

EBF 抗体 (C-8) Alexa Fluor® 647

sc-137065 AF647
200 µg/ml
RMB2685.00

EBF 抗体 (C-8) Alexa Fluor® 680

sc-137065 AF680
200 µg/ml
RMB2685.00

EBF 抗体 (C-8) Alexa Fluor® 790

sc-137065 AF790
200 µg/ml
RMB2685.00

Does it react with mouse EBF1?

Asked by: gcomai
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

I am using EBF (C-8): sc-137065 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 7
Date published: 2017-01-24
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Rated 5 out of 5 by from Excellent for IFTest IF (1:1000 with antigen retrieval) on frozen section from 4%PFA fixed embryonic mouse brain. It gives clear, specific signal.
Date published: 2019-01-03
Rated 4 out of 5 by from Working EBF antibody for WBWorking EBF antibody for WB. Clean background with one or two non-specific bands.
Date published: 2017-10-18
Rated 4 out of 5 by from Verified in Ebf1 KO sampleEbf1 KO mouse brain tissue lysates. There is one band deficiency at around 63 kDa in Ebf1-/- sample. But there are many bands.
Date published: 2017-09-29
Rated 5 out of 5 by from GreatI ordered this antibody and test this antibody with overexpressing EBF1 and endogenous protein. It work great! I got the specific and clear band.
Date published: 2017-08-14
Rated 5 out of 5 by from Awesome antibodyEBF Antibody (C-8) is great for WB and IHC! It produced positive and specific signal
Date published: 2017-01-21
Rated 5 out of 5 by from Clean blot with single strong band at expectedClean blot with single strong band at expected size using Ramos and IMR-32 whole cell lysates. -SCBT QC
Date published: 2015-07-17
Rated 5 out of 5 by from Band detected at expected mw in human EBF1Band detected at expected mw in human EBF1 transfected 293T whole cell lysates and Ramos nuclear extract. -SCBT QC
Date published: 2015-07-06
Rated 5 out of 5 by from ChIP publishable data with human GM12878 cellsChIP publishable data with human GM12878 cells - SCBT Publication Review
Date published: 2015-05-01
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EBF 抗体 (C-8) is rated 4.8 out of 5 by 9.
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