Date published: 2025-9-7
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E2F-2 Antibody (TFE-25) - Western Blotting - Image 395165
E2F-2 抗体 (TFE-25) HRP: sc-9967 HRP. KNRK 核提取物 (A), PC-12 (B) 和 Ramos (C) 全细胞裂解液中 E2F-2 表达的直接 Western 印迹分析.
E2F-2 抗体 (TFE-25) HRP: sc-9967 HRP. KNRK 核提取物 (A), PC-12 (B) 和 Ramos (C) 全细胞裂解液中 E2F-2 表达的直接 Western 印迹分析.

E2F-2 抗体 (TFE-25): sc-9967

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说明书
  • E2F-2 抗体 TFE-25 是小鼠单克隆 IgG1 κ，E2F-2抗体, 在39篇文献中引用，规格为200 µg/ml
  • 针对全长E2F-2，human起源
  • E2F-2 抗体 (TFE-25) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 E2F-2
  • 抗E2F-2抗体（TFE-25）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-9967 X, 200 µg/0.1 ml)
  • 关于如何获取E2F-2 (TFE-25): sc-9967的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 E2F-2 抗体 (TFE-25) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与E2F-2 抗体 (TFE-25) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 E2F 抗体 包括 E2F-1, E2F-2, E2F-3, E2F-4, E2F-5, E2F-6和E2F-8

E2F-2抗体（TFE-25）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的E2F-2蛋白。E2F-2单克隆抗体（TFE-25）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合物。E2F-2通过控制DNA合成和细胞增殖所需的基因表达，在调节细胞周期中发挥着关键作用，特别是在从G1期到S期的过渡中。这种调节对于维持正常的细胞生长和分裂至关重要，因为失调会导致细胞无序增殖，并导致肿瘤形成。E2F-2是一种转录因子，可与DP-1蛋白形成异二聚体，从而形成活跃的E2F转录调控复合物，影响靶基因的表达。E2F-2在细胞周期调节中的重要性凸显了其作为癌症和其他增生性疾病治疗干预靶标的潜力。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

E2F-2 抗体 (TFE-25) 参考文献:

  1. 转录因子 E2F-2 与内皮素转换酶-1b 启动子的不同结合影响血压调节。  |  Funke-Kaiser, H., et al. 2003. Hum Mol Genet. 12: 423-33. PMID: 12566389
  2. 编码具有转录因子 E2F 特性的 pRB 结合蛋白的 cDNA。  |  Helin, K., et al. 1992. Cell. 70: 337-50. PMID: 1638634
  3. E2F 转录因子是 RB 蛋白的细胞靶标。  |  Chellappan, SP., et al. 1991. Cell. 65: 1053-61. PMID: 1828392
  4. 视网膜母细胞瘤产物的 T/E1A 结合域可选择性地与序列特异性 DNA 结合蛋白相互作用。  |  Chittenden, T., et al. 1991. Cell. 65: 1073-82. PMID: 1828394
  5. M2巨噬细胞衍生的外泌体通过靶向E2F2促进胰腺导管腺癌的血管生成和生长  |  Yang, Y., et al. 2021. Mol Ther. 29: 1226-1238. PMID: 33221435
  6. 非典型 E3 连接酶 ZFP91 可促进小分子诱导的 E2F2 转录因子降解，从而用于癌症治疗。  |  Liu, TT., et al. 2022. EBioMedicine. 86: 104353. PMID: 36375317
  7. E2F2 在癌症进展中的作用及其作为治疗靶点的价值。  |  Gao, Y., et al. 2024. Front Immunol. 15: 1397303. PMID: 38807594
  8. E2F-4是E2F转录因子家族的新成员，与p107相互作用。  |  Ginsberg, D., et al. 1994. Genes Dev. 8: 2665-79. PMID: 7958924
  9. E2F-4是E2F基因家族的新成员，具有致癌活性，并在体内与p107结合。  |  Beijersbergen, RL., et al. 1994. Genes Dev. 8: 2680-90. PMID: 7958925
  10. 与 DNA 和视网膜母细胞瘤基因产物的结合由不同 E2F 家族成员形成的复合物促进。  |  Krek, W., et al. 1993. Science. 262: 1557-60. PMID: 8248803
  11. 转录因子 E2F-1 和 DP-1 的异源二聚化导致了合作性的转激活。  |  Helin, K., et al. 1993. Genes Dev. 7: 1850-61. PMID: 8405995
  12. E2F-6 是 E2F 家族的成员，可作为转录抑制因子。  |  Trimarchi, JM., et al. 1998. Proc Natl Acad Sci U S A. 95: 2850-5. PMID: 9501179

订购信息

产品名称产品编号规格价格数量收藏夹

E2F-2 抗体 (TFE-25)

sc-9967
200 µg/ml
RMB2377.00

E2F-2 (TFE-25): m-IgG Fc BP-HRP 套装

sc-526528
200 µg Ab; 10 µg BP
RMB2662.00

E2F-2 (TFE-25): m-IgG1 BP-HRP 套装

sc-531901
200 µg Ab; 20 µg BP
RMB2662.00

E2F-2 抗体 (TFE-25) X

sc-9967 X
200 µg/0.1 ml
RMB2377.00

E2F-2 抗体 (TFE-25) AC

sc-9967 AC
500 µg/ml, 25% agarose
RMB3129.00

E2F-2 抗体 (TFE-25) HRP

sc-9967 HRP
200 µg/ml
RMB2377.00

E2F-2 抗体 (TFE-25) FITC

sc-9967 FITC
200 µg/ml
RMB2482.00

E2F-2 抗体 (TFE-25) PE

sc-9967 PE
200 µg/ml
RMB2580.00

E2F-2 抗体 (TFE-25) Alexa Fluor® 488

sc-9967 AF488
200 µg/ml
RMB2685.00

E2F-2 抗体 (TFE-25) Alexa Fluor® 546

sc-9967 AF546
200 µg/ml
RMB2685.00

E2F-2 抗体 (TFE-25) Alexa Fluor® 594

sc-9967 AF594
200 µg/ml
RMB2685.00

E2F-2 抗体 (TFE-25) Alexa Fluor® 647

sc-9967 AF647
200 µg/ml
RMB2685.00

E2F-2 抗体 (TFE-25) Alexa Fluor® 680

sc-9967 AF680
200 µg/ml
RMB2685.00

E2F-2 抗体 (TFE-25) Alexa Fluor® 790

sc-9967 AF790
200 µg/ml
RMB2685.00

Dear, i would like to know which is the light chain of sc9967 antibody. thanks,

Asked by: dmestre
Thank you for your question. We have not yet determined the specific light chain type for this mouse monoclonal antibody, E2F-2 (TFE-25): sc-9967. However, because >98% of all mouse IgG has a kappa light chain, this is more than likely the light chain type. If you would like to test a free 10 µg sample of our mouse IgG kappa chain binding protein, please contact our Technical Service Department.
Answered by: Technical Support
Date published: 2017-05-17

When should the TransCruz reagent sc-9967 X be used instead of the standard form sc-9967?

Asked by: Trav11
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Didnot work for WBdid not work for western - samples were human cells in culture
Date published: 2017-11-21
Rated 5 out of 5 by from high expressionWhen using 1/200 dilutions, it works well in Ramos whole cell lysate, thanks to Santa antibodies.
Date published: 2017-10-26
Rated 5 out of 5 by from expected signalThis antibody was used to analyisis E2F-2 in breast cancer tissue lysates. We got expected results by WB (1:600), we found E2F-2 is related to the occurrence and development of breast cancer, and may be a marker of tumor proliferation in breast cancer.
Date published: 2017-09-09
Rated 5 out of 5 by from good for westernThe specific bands were detected by Western blot and 1:500 in THP1.
Date published: 2017-03-08
Rated 5 out of 5 by from Worked well for WB and ChIP using human SW13Worked well for WB and ChIP using human SW13, TSUPr-1, U2OS, and A5-1 whole cell lysates. -SCBT Publication Review
Date published: 2015-04-23
Rated 5 out of 5 by from Strong band observed in Western Blot analysisStrong band observed in Western Blot analysis of PMA-treated A-431, NIH/3T3 and KNRK nuclear extracts. -SCBT QC
Date published: 2015-04-02
Rated 5 out of 5 by from Pubilshed results of IP and WB using murinePubilshed results of IP and WB using murine Intestinal epithelium extract. -SCBT Publication Review
Date published: 2015-02-17
Rated 5 out of 5 by from Produced great immunoperoxidase nuclear andProduced great immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human placenta tissue. -SCBT QC
Date published: 2015-01-21
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E2F-2 抗体 (TFE-25) is rated 4.6 out of 5 by 9.
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