Date published: 2025-9-7

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E2A 抗体 (Yae): sc-416

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说明书
  • E2A抗体(Yae)是小鼠单克隆IgG1 κ, 在66篇文献中引用,规格为200 µg/ml
  • 对应于195-208的表位,在E2A的human起源区域中映射,在E47和E12(泛-2)之间保守
  • 抗-E2A 抗体 (Yae) 推荐用于 WB, IP 和 IF,检测mouse, rat 和human 来源的 ITF-1, and E2A isoforms E12 and E47
  • 抗E2A抗体(Yae)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-416 X, 200 µg/0.1 ml)
  • 关于如何获取E2A (Yae): sc-416免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP是E2A Antibody (Yae) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与E2A Antibody (Yae) 打包提供(请参阅下面的订购信息)。

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関連項目

E2A抗体(Yae)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)和免疫荧光(IF)检测小鼠、大鼠和人类样本中的E2A蛋白(也称为ITF-1)。E2A单克隆抗体(Yae)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合物。E2A蛋白是螺旋-环-螺旋转录因子家族的一员,在B细胞成熟和肌分化过程中调节基因表达方面发挥着至关重要的作用。E2A蛋白对于与Myo D和其他基本螺旋-环-螺旋蛋白形成异二聚体至关重要,这些蛋白与肌肉特异性基因调控区域中的E盒元件结合。这种相互作用对于肌肉细胞的正常分化至关重要,因为E2A蛋白可确保激活肌肉发育所需的基因。E2A蛋白可形成同源和异源寡聚体,从而精确调控基因表达,使E2A蛋白成为谱系特异性转录调控的关键参与者。E2A单克隆抗体(Yae)使研究人员能够深入了解肌肉细胞分化的机制以及转录因子相互作用在各种生物学过程中的更广泛影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

E2A 抗体 (Yae) 参考文献:

  1. HEB,一种与 E2A 和 ITF2 相关的螺旋环螺旋蛋白,可调节肌原调节因子的 DNA 结合能力。  |  Hu, JS., et al. 1992. Mol Cell Biol. 12: 1031-42. PMID: 1312219
  2. 螺旋环螺旋肽的分子特征。  |  Anthony-Cahill, SJ., et al. 1992. Science. 255: 979-83. PMID: 1312255
  3. 老龄 B 细胞前体中 E47 蛋白的 Notch 依赖性降解加速与 ERK MAPK 激活增加缔合作用有关。  |  King, AM., et al. 2007. J Immunol. 178: 3521-9. PMID: 17339447
  4. Herculin是MyoD肌生成调控基因家族的第四个成员。  |  Miner, JH. and Wold, B. 1990. Proc Natl Acad Sci U S A. 87: 1089-93. PMID: 2300571
  5. 异源螺旋环-螺旋蛋白之间的相互作用产生了能与共同 DNA 序列特异性结合的复合物。  |  Murre, C., et al. 1989. Cell. 58: 537-44. PMID: 2503252
  6. 肌生成素是一种调节肌生成的因子,其结构域与 MyoD 同源。  |  Wright, WE., et al. 1989. Cell. 56: 607-17. PMID: 2537150
  7. 鉴定 MRF4:肌肉调节因子基因家族的新成员。  |  Rhodes, SJ. and Konieczny, SF. 1989. Genes Dev. 3: 2050-61. PMID: 2560751
  8. 一种与 MyoD1 相关但不同于 MyoD1 的新型人类肌肉因子可诱导 10T1/2 成纤维细胞的成肌转换。  |  Braun, T., et al. 1989. EMBO J. 8: 701-9. PMID: 2721498
  9. 转录因子 TEAD2 和 E2A 在全球范围内抑制乙酰-CoA 的合成,从而促进肿瘤发生。  |  Park, S., et al. 2022. Mol Cell. 82: 4246-4261.e11. PMID: 36400009
  10. E2A-PBX1 融合在 B 细胞急性淋巴细胞白血病中的进展。  |  Yang, M., et al. 2024. Ann Hematol. 103: 3385-3398. PMID: 38148344
  11. E2A 在 B 细胞发育中的功能  |  Miyazaki, M. and Miyazaki, K. 2024. Adv Exp Med Biol. 1459: 97-113. PMID: 39017841
  12. E2A 基因编码的螺旋环螺旋转录因子的细胞特异性表达。  |  Aronheim, A., et al. 1993. Nucleic Acids Res. 21: 1601-6. PMID: 8479911

订购信息

产品名称产品编号规格价格数量收藏夹

E2A 抗体 (Yae)

sc-416
200 µg/ml
RMB2377.00

E2A (Yae): m-IgG1 BP-HRP 套装

sc-531623
200 µg Ab; 20 µg BP
RMB2662.00

E2A 抗体 (Yae) X

sc-416 X
200 µg/0.1 ml
RMB2377.00

E2A 抗体 (Yae) AC

sc-416 AC
500 µg/ml, 25% agarose
RMB3129.00

E2A 抗体 (Yae) HRP

sc-416 HRP
200 µg/ml
RMB2377.00

E2A 抗体 (Yae) FITC

sc-416 FITC
200 µg/ml
RMB2482.00

E2A 抗体 (Yae) PE

sc-416 PE
200 µg/ml
RMB2580.00

E2A 抗体 (Yae) Alexa Fluor® 488

sc-416 AF488
200 µg/ml
RMB2685.00

E2A 抗体 (Yae) Alexa Fluor® 546

sc-416 AF546
200 µg/ml
RMB2685.00

E2A 抗体 (Yae) Alexa Fluor® 594

sc-416 AF594
200 µg/ml
RMB2685.00

E2A 抗体 (Yae) Alexa Fluor® 647

sc-416 AF647
200 µg/ml
RMB2685.00

E2A 抗体 (Yae) Alexa Fluor® 680

sc-416 AF680
200 µg/ml
RMB2685.00

E2A 抗体 (Yae) Alexa Fluor® 790

sc-416 AF790
200 µg/ml
RMB2685.00

Good afternnon, we are interested in this E2A antibody. Could I ask the name of clone and if it can be used for FC? If yes, is it the conjugated version? Thank you.

Asked by: Anonymous
Here is a list that we offer for our E2A antibodies and their conjugations. https://www.scbt.com/browse/e2a-Antibodies/_/N-1wkm1w8
Answered by: Technical Service
Date published: 2023-08-24

When should the TransCruz reagent sc-416 X be used instead of the standard form sc-416?

Asked by: Trav11
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2023-08-22
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Rated 1 out of 5 by from Bad for IFWe used this antibody in immunostaining. But it didn't work well.
Date published: 2021-12-07
Rated 5 out of 5 by from Good for WBThis antibody is good for Western blot using human cell nuclear extract.
Date published: 2018-10-16
Rated 5 out of 5 by from Great detectionThis is a reputable antibody that provides good data in WB procedures. It is reactive with mouse, rat, and human and has been used to publish nearly 50 times.
Date published: 2017-02-02
Rated 5 out of 5 by from We are using this antibody in western blotWe are using this antibody in western blot with good success. Publication review
Date published: 2015-11-27
Rated 5 out of 5 by from Good ChIP antibodyGood ChIP antibody, published using mouse bone marrow cells. -SCBT Publication Review
Date published: 2015-03-24
Rated 5 out of 5 by from Great antibody for EMSAGreat antibody for EMSA, tested with mouse WEHI-231 NE. -SCBT Publication Review
Date published: 2015-03-14
Rated 5 out of 5 by from Great monoGreat mono! Single strong band observed in CRISPR control and complete knockout in sc-423303 transfected cells. -SCBT QC
Date published: 2015-03-11
Rated 5 out of 5 by from Works well for Western blot with Jurkat wholeWorks well for Western blot with Jurkat whole cell lysates. -SCBT QC
Date published: 2015-01-27
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E2A抗体 (Yae) is rated 4.6 out of 5 by 9.
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