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dystrophin Antibody (MANDRA1) - Immunohistochemistry - Image 290458
肌营养不良蛋白抗体 (MANDRA1): sc-73592. 福尔马林固定, 石蜡包埋的人心肌组织的免疫过氧化物酶染色, 显示心肌细胞的膜和胞浆染色.
肌营养不良蛋白抗体 (MANDRA1): sc-73592. 福尔马林固定, 石蜡包埋的人心肌组织的免疫过氧化物酶染色, 显示心肌细胞的膜和胞浆染色.

dystrophin 抗体 (MANDRA1): sc-73592

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说明书
  • dystrophin 抗体 MANDRA1 是小鼠单克隆 IgG1 κ，dystrophin抗体, 在10篇文献中引用，规格为200 µg/ml
  • 针对氨基酸3200-3684 dystrophin human
  • 推荐用于 mouse, rat， human 和 fish 来源的an epitope corresponding to amino acids 3558-3684 of dystrophin WB, IP, IF 和 IHC(P)检测
  • 还可偶联琼脂糖， 用于 IP；偶联HRP，用于WB, IHC(P) 和 ELISA; 偶联FITC以及荧光素，用于IF, IHC(P) 和 FCM
  • 还可偶联Alexa Fluor® 488 和 Alexa Fluor® 647，用于IF, IHC(P) 和 FCM
  • 关于如何获取dystrophin (MANDRA1): sc-73592的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 dystrophin 抗体 (MANDRA1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与dystrophin 抗体 (MANDRA1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Dystrophin Antibody (MANDRA1) 是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠和人类来源的肌营养不良蛋白。抗肌营养不良蛋白抗体（MANDRA1）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。肌营养不良蛋白在肌营养不良蛋白-糖蛋白复合物（DGC）中起着至关重要的作用，该复合物将肌纤维中的F-肌动蛋白细胞骨架与细胞外基质连接起来，从而在收缩过程中保持肌肉细胞的完整性。这种连接对于肌肉功能至关重要，因为它有助于稳定肌肉膜，防止在机械应力下受损。肌营养不良蛋白缺乏症与杜兴氏（DMD）和贝克尔（BMD）肌肉营养不良症等严重肌肉疾病有关，这凸显了肌营养不良蛋白在肌肉健康中的重要性。人类肌营养不良蛋白基因跨越2.4兆碱基，包含80多个外显子，并产生14kb的mRNA，该mRNA可进行差异剪接以产生多种转录本，每个转录本编码不同的蛋白质同工型。肌营养不良蛋白约占横纹肌蛋白总量的0.002%，存在于骨骼肌的三联结处，据信肌营养不良蛋白在钙离子稳态和力传递中发挥着重要作用，进一步凸显了肌营养不良蛋白在肌肉生理学中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

dystrophin 抗体 (MANDRA1) 参考文献:

  1. 肌营养不良蛋白是一种微管相关蛋白。  |  Prins, KW., et al. 2009. J Cell Biol. 186: 363-9. PMID: 19651889
  2. 使用鸡尾酒反义寡核苷酸跳过肌营养不良蛋白转录物的多个外显子。  |  Echigoya, Y. and Yokota, T. 2014. Nucleic Acid Ther. 24: 57-68. PMID: 24380394
  3. 肌营养不良蛋白定量：生物学和转化研究的意义。  |  Anthony, K., et al. 2014. Neurology. 83: 2062-9. PMID: 25355828
  4. 临床试验中的肌营养不良蛋白分析。  |  Aartsma-Rus, A. 2014. J Neuromuscul Dis. 1: 41-53. PMID: 27858668
  5. Utrophin补偿小鼠精子发生过程中dystrophin的缺失。  |  Chen, HC., et al. 2017. Sci Rep. 7: 7372. PMID: 28785010
  6. Dystrophin Dp71 亚型定位到人体细胞的线粒体。  |  Niba, ETE., et al. 2021. Life (Basel). 11: PMID: 34575126
  7. 新生大鼠和成年大鼠肠道中的肌营养不良蛋白  |  Lionarons, JM., et al. 2021. Life (Basel). 11: PMID: 34833031
  8. 大脑中肌萎缩蛋白同工酶和相互作用酶的作用。  |  Tetorou, K., et al. 2024. Brain.. PMID: 39673425
  9. 肌肉萎缩症中的肌营养不良蛋白相关蛋白。  |  Ozawa, E., et al. 1995. Hum Mol Genet. 4 Spec No: 1711-6. PMID: 8541869
  10. 肌营养不良蛋白和肌营养不良蛋白的结构-功能关系  |  Winder, SJ. 1996. Biochem Soc Trans. 24: 497-501. PMID: 8736791

订购信息

产品名称产品编号规格价格数量收藏夹

dystrophin 抗体 (MANDRA1)

sc-73592
200 µg/ml
RMB2377.00

dystrophin (MANDRA1): m-IgG Fc BP-HRP 套装

sc-527006
200 µg Ab; 10 µg BP
RMB2662.00

dystrophin (MANDRA1): m-IgG1 BP-HRP 套装

sc-532379
200 µg Ab; 20 µg BP
RMB2662.00

dystrophin 抗体 (MANDRA1) AC

sc-73592 AC
500 µg/ml, 25% agarose
RMB3129.00

dystrophin 抗体 (MANDRA1) HRP

sc-73592 HRP
200 µg/ml
RMB2377.00

dystrophin 抗体 (MANDRA1) FITC

sc-73592 FITC
200 µg/ml
RMB2482.00

dystrophin 抗体 (MANDRA1) PE

sc-73592 PE
200 µg/ml
RMB2580.00

dystrophin 抗体 (MANDRA1) Alexa Fluor® 488

sc-73592 AF488
200 µg/ml
RMB2685.00

dystrophin 抗体 (MANDRA1) Alexa Fluor® 647

sc-73592 AF647
200 µg/ml
RMB2685.00

can i use dystrophin Antibody (MANDRA1): sc-73592 to IP the dystrophin from the rabbit muscle?

Asked by: bingo
Thank you for your question. Yes sc-73592 can be used for IP. https://www.scbt.com/resources/protocols/immunoprecipitation-western-blots
Answered by: BlakeJ
Date published: 2023-05-31

Can dystrophin (MANDRA1): sc-73592 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, dystrophin (MANDRA1): sc-73592 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from recognizes Dp71 isoform of dystrophinwe were interested in this sample to detect Dp71 isoform of dystrophin. It works fine in WB, IP and IF. for WB the final concentration was 1:1000 (or 0.2ug/ml) in TBST. For blocking I used 5% milk in TBST. For IP I used 1ug of AB and 2mg of lysate. It also works fine in coIP. for IF I used 1:100 final dilution of AB.
Date published: 2018-08-27
Rated 5 out of 5 by from Great ！This antibody (sc-73592, 1:333 dilution) works well with dystrophin proteins in skeletal muscle tissues, including Quadriceps (Q), Gastrocnemius (G) and Tibialis Anterior (T), of normal mouse. Mdx tissue lysates are negative controls.
Date published: 2017-09-05
Rated 5 out of 5 by from Great for Paraffin Sections!Excellent choice when working with paraffin-embedded sections in IHC.
Date published: 2017-01-18
Rated 5 out of 5 by from Excellent immunoperoxidase membrane and cytoplasmicExcellent immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human heart muscle tissue. -SCBT QC
Date published: 2015-04-06
Rated 5 out of 5 by from Band of interest observed in WB in both humanBand of interest observed in WB in both human skeletal muscle and rat skeletal muscle issue extracts. -SCBT QC
Date published: 2015-03-31
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dystrophin 抗体 (MANDRA1) is rated 4.8 out of 5 by 5.
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