Date published: 2025-9-8
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Dyskerin Antibody (C-11) - Western Blotting - Image 355524
Dyskerin 抗体 (C-11): sc-365731. HeLa 核提取物 (A), HCT-116 (B), Hep G2 (C) 和 SW480 (D) 全细胞裂解液中 Dyskerin 表达的 Western 印迹分析.
Dyskerin 抗体 (C-11): sc-365731. HeLa 核提取物 (A), HCT-116 (B), Hep G2 (C) 和 SW480 (D) 全细胞裂解液中 Dyskerin 表达的 Western 印迹分析.

Dyskerin 抗体 (C-11): sc-365731

4.3(3)
寫評論提問

说明书
  • Dyskerin抗体(C-11)是小鼠单克隆IgG1 κ, 在2篇文献中引用，规格为200 µg/ml
  • 针对human起源的Dyskerin内部区域内的氨基酸171-470进行
  • 推荐用于 mouse, rat 和 human 来源的Dyskerin WB, IP, IF 和 ELISA检测
  • 可用于ChIP的TransCruz试剂(sc-365731 X, 200 µg/0.1 ml)
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是Dyskerin Antibody (C-11) for WB applications. 的首选辅助检测试剂，这些试剂现与Dyskerin Antibody (C-11) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Dyskerin（NAP57）与伴侣蛋白Nopp140结合，并与GAR1（NOLA1）、NHP2（NOLA2）和Nop10形成小核糖核蛋白颗粒，用于将尿苷异构化为假尿苷。GAR1、NHP2和dyskerin定位于核仁的致密纤维组分和核Cajal体中。Dyskerin基因定位于Xq28染色体。Dyskerin基因中的错义突变会干扰dyskerin的正常核定位，并导致先天性角化不良（DKC）。DKC是一种罕见的X连锁骨髓疾病，其特征是皮肤色素沉着过度、指甲营养不良、睾丸萎缩和口腔黏膜白斑。GAR1基因定位于4q25染色体。NHP2基因定位于5q35.3染色体，并编码一个由155个氨基酸组成的蛋白质。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

Dyskerin 抗体 (C-11)

sc-365731
200 µg/ml
RMB2377.00

Dyskerin (C-11): m-IgG Fc BP-HRP 套装

sc-540362
200 µg Ab; 10 µg BP
RMB2662.00

Dyskerin (C-11): m-IgG1 BP-HRP 套装

sc-542069
200 µg Ab; 20 µg BP
RMB2662.00

Dyskerin 抗体 (C-11) X

sc-365731 X
200 µg/0.1 ml
RMB2377.00

Can this antibody detect both isoforms of dyskerin (isoform-1 and isoform-3)? In the WB images, 1 image shows two bands (presumably corresponding to the 2 isoforms) when analyzing HeLa nuclear extract, but others only show one band for the same sample.

Asked by: allie24
Thank you for your question. Dyskerin (C-11) can detect isoform 1 and 3. https://www.uniprot.org/uniprotkb/O60832/entry
Answered by: BlakeJ
Date published: 2024-09-26

该抗体，做WB实验，心脏组织出条带。为什么原代心肌细胞，成纤维细胞，不出条带？我用的RIPA裂解液，加了SDS,磷酸酶抑制剂，蛋白酶抑制剂。

Asked by: realistic
感谢您的提问。您可以拨打电话：021-60936350，发送邮件到[email protected]，或者我们官网上的在线聊天，联系我们的亚洲技术支持团队，了解更多信息。 Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Jiajun Z
Date published: 2024-07-26

What is the recommended fixation method for immunofluorescence staining with Dyskerin (C-11): sc-365731 monoclonal antibody?

Asked by: cjMara
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Produced exceptional immunofluorescence nucleolarProduced exceptional immunofluorescence nucleolar staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-08
Rated 4 out of 5 by from Band of interest seen in WB using HeLa nuclearBand of interest seen in WB using HeLa nuclear extract. -SCBT QC
Date published: 2015-03-22
Rated 4 out of 5 by from Good band detected in WB using Raji wholeGood band detected in WB using Raji whole cell lysate. -SCBT QC
Date published: 2015-02-28
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Dyskerin 抗体 (C-11) is rated 4.3 out of 5 by 3.
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