Date published: 2025-9-8
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Dynein IC1/2, cytosolic Antibody (74-1) - Western Blotting - Image 354364
Dynein IC1/2, cytosolic 抗体 (74-1) HRP: sc-13524 HRP. 在 HeLa (A) 和 U-87 MG (B) 全细胞裂解液以及小鼠脑 (C) 和大鼠脑 (D) 组织提取物中直接对 Dynein IC1/2 (细胞质) 的表达进行 Western 印迹分析.
Dynein IC1/2, cytosolic 抗体 (74-1) HRP: sc-13524 HRP. 在 HeLa (A) 和 U-87 MG (B) 全细胞裂解液以及小鼠脑 (C) 和大鼠脑 (D) 组织提取物中直接对 Dynein IC1/2 (细胞质) 的表达进行 Western 印迹分析.

Dynein IC1/2, cytosolic 抗体 (74-1): sc-13524

5.0(11)
寫評論提問

说明书
  • Dynein IC1/2, cytosolic 抗体 74-1 是小鼠单克隆 IgG2b κ，Dynein IC1/2, cytosolic抗体, 在77篇文献中引用，规格为200 µg/ml
  • 针对bovine来源的纯化脑组织细胞质动力蛋白
  • Dynein IC1/2, cytosolic 抗体 (74-1) 推荐用于WB, IP, IF 和 FCM，检测broad species 来源的 intermediate chains 1 and 2 of cytosolic Dynein
  • 抗Dynein IC1/2, cytosolic抗体（74-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Dynein IC1/2, cytosolic (74-1): sc-13524的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是Dynein IC1/2, cytosolic Antibody (74-1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Dynein IC1/2, cytosolic Antibody (74-1) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Dynein抗体，包括Dynein HC, Dynein IC1, cytosolic, Dynein IC2, axonemal, Dynein IC2, cytosolic, DYNLL1和DYNLL2

细胞质抗体（74-1）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和流式细胞术（FCM）检测多种物种来源的细胞质蛋白Dynein IC1/2。细胞质（74-1）驱动蛋白IC1/2抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。动力蛋白是重要的多亚基ATP酶，通过与微管相互作用，促进细胞货物向细胞中心移动，在细胞运输中发挥着关键作用。Dynein IC1和IC2蛋白是细胞质中动力蛋白复合物的组成部分，该复合物由大约12个亚基组成，包括两个重链和两个中间链，它们将动力蛋白锚定到其货物上。该复合物对于维持细胞完整性和功能至关重要的各种细胞功能（如细胞器运输和中心体组装）至关重要。动力蛋白的羧基末端对于微管依赖性运动尤为重要，并且在物种间高度保守，而氨基末端区域则表现出更大的变异性，从而实现功能多样性。此外，动力蛋白的活性受多种蛋白调节，包括动力蛋白、LIS1和NudEL（NudE样），它们对于动力蛋白马达在细胞过程中的正常运作至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Dynein IC1/2, cytosolic 抗体 (74-1) 参考文献:

  1. 细胞质动力蛋白在负载时发挥齿轮的功能。  |  Mallik, R., et al. 2004. Nature. 427: 649-52. PMID: 14961123
  2. 细胞质动力蛋白对微管进行核化，从而在体内将微管组织成径向阵列。  |  Malikov, V., et al. 2004. Mol Biol Cell. 15: 2742-9. PMID: 15047865
  3. 动力蛋白重链家族  |  Asai, DJ. and Wilkes, DE. 2004. J Eukaryot Microbiol. 51: 23-9. PMID: 15068262
  4. 外臂动力蛋白产生的轴突微管高速滑动。  |  Seetharam, RN. and Satir, P. 2005. Cell Motil Cytoskeleton. 60: 96-103. PMID: 15605357
  5. 动力蛋白功能的卸载模型：运动亚基的不同功能。  |  Lee, WL., et al. 2005. J Cell Biol. 168: 201-7. PMID: 15642746
  6. 细胞质动力蛋白在微管和神经丝轴突运输中的作用  |  He, Y., et al. 2005. J Cell Biol. 168: 697-703. PMID: 15728192
  7. NudEL 通过 LIS1 将动力蛋白定向到微管末端。  |  Li, J., et al. 2005. Nat Cell Biol. 7: 686-90. PMID: 15965467
  8. 细胞质动力蛋白命名法。  |  Pfister, KK., et al. 2005. J Cell Biol. 171: 411-3. PMID: 16260502
  9. Dynein 运动：四个头比两个头好。  |  McGrath, JL. 2005. Curr Biol. 15: R970-2. PMID: 16332531
  10. 细胞质动力蛋白亚基家族的遗传分析。  |  Pfister, KK., et al. 2006. PLoS Genet. 2: e1. PMID: 16440056

订购信息

产品名称产品编号规格价格数量收藏夹

Dynein IC1/2, cytosolic 抗体 (74-1)

sc-13524
200 µg/ml
RMB2377.00

Dynein IC1/2, cytosolic (74-1): m-IgG Fc BP-HRP 套装

sc-528285
200 µg Ab; 10 µg BP
RMB2662.00

Dynein IC1/2, cytosolic (74-1): m-IgGκ BP-HRP 套装

sc-520624
200 µg Ab, 40 µg BP
RMB2662.00

Dynein IC1/2, cytosolic (74-1): m-IgG2b BP-HRP 套装

sc-550023
200 µg Ab; 10 µg BP
RMB2662.00

Dynein IC1/2, cytosolic 抗体 (74-1) AC

sc-13524 AC
500 µg/ml, 25% agarose
RMB3129.00

Dynein IC1/2, cytosolic 抗体 (74-1) HRP

sc-13524 HRP
200 µg/ml
RMB2377.00

Dynein IC1/2, cytosolic 抗体 (74-1) FITC

sc-13524 FITC
200 µg/ml
RMB2482.00

Dynein IC1/2, cytosolic 抗体 (74-1) PE

sc-13524 PE
200 µg/ml
RMB2580.00

Dynein IC1/2, cytosolic 抗体 (74-1) Alexa Fluor® 488

sc-13524 AF488
200 µg/ml
RMB2685.00

Dynein IC1/2, cytosolic 抗体 (74-1) Alexa Fluor® 546

sc-13524 AF546
200 µg/ml
RMB2685.00

Dynein IC1/2, cytosolic 抗体 (74-1) Alexa Fluor® 594

sc-13524 AF594
200 µg/ml
RMB2685.00

Dynein IC1/2, cytosolic 抗体 (74-1) Alexa Fluor® 647

sc-13524 AF647
200 µg/ml
RMB2685.00

Dynein IC1/2, cytosolic 抗体 (74-1) Alexa Fluor® 680

sc-13524 AF680
200 µg/ml
RMB2685.00

Dynein IC1/2, cytosolic 抗体 (74-1) Alexa Fluor® 790

sc-13524 AF790
200 µg/ml
RMB2685.00

Would the antibody sc-13524 detect porcine dynein?

Asked by: DinaA
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-03-09

What is the recommended secondary for this mouse monoclonal primary?

Asked by: AbPolly
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-20

Can this antibody be used with Xenopus samples?

Asked by: AbPolly
This antibody is recommended for use with a broad range of species. For specific species, please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data.
Answered by: Technical Support 14
Date published: 2019-03-04
  • y_2025, m_8, d_30, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_zh_CN, sid_13524, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 133ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Great antibodyIt seems the best commercial DIC antibody which can be used for both WB and IP. Excellent.
Date published: 2020-11-17
Rated 5 out of 5 by from Good for IFThis Dynein IC1/2, cytosolic Antibody (74-1), sc-13524, shows strong specific signal in IF staining in primary rat motor neuron.
Date published: 2018-02-20
Rated 5 out of 5 by from Works well for Western BlotWe used this antibody in CV-1(monkey) cell line after performing a western blot. 1:2000 in milk. The bands were clear with very little background.
Date published: 2017-11-01
Rated 5 out of 5 by from Great productOutstanding product for both western blot and IF staining applications
Date published: 2017-01-20
Rated 5 out of 5 by from Strong signalThis Dynein IC1/2, cytosolic antibody shows strong signal in WB.
Date published: 2017-01-12
Rated 5 out of 5 by from Great in WB!!!Detects a single band in HeLa lysate at a 1:500 dilution. VERY strong single.
Date published: 2016-11-29
Rated 5 out of 5 by from Positive western blot detection of Dynein IC1/2Positive western blot detection of Dynein IC1/2, cytosolic expression in T98G, IMR-32 and L8 whole cell lysates. -SCBT QC
Date published: 2015-07-23
Rated 5 out of 5 by from Great intracellular FCM data of fixed andGreat intracellular FCM data of fixed and permeabilized L8 cells. -SCBT QC
Date published: 2015-06-01
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_11
  • loc_zh_CN, sid_13524, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 18ms
  • REVIEWS, PRODUCT
Dynein IC1/2, cytosolic 抗体 (74-1) is rated 5.0 out of 5 by 11.
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_11
  • loc_zh_CN, sid_13524, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 120ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。