Date published: 2025-9-7
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Dvl-1/Dishevelled 1/DVL1 Antibody (3F12) - Immunohistochemistry - Image 156644
Dvl-1 抗体 (3F12): sc-8025. 对福尔马林固定, 石蜡包埋的人心肌组织进行免疫过氧化物酶染色, 显示心肌细胞的胞浆染色.
Dvl-1/Dishevelled 1/DVL1 Antibody (3F12) - Immunohistochemistry - Image 156644
Dvl-1 抗体 (3F12): sc-8025. 对福尔马林固定, 石蜡包埋的人心肌组织进行免疫过氧化物酶染色, 显示心肌细胞的胞浆染色.
Dvl-1/Dishevelled 1/DVL1 Antibody (3F12) - Western Blotting - Image 3417
Dvl-1 抗体 (3F12): sc-8025. SK-N-SH (A) 和 KNRK (B) 全细胞裂解液中 Dvl-1 表达的 Western 印迹分析.
Dvl-1/Dishevelled 1/DVL1 Antibody (3F12) - Western Blotting - Image 378856
Dvl-1 抗体 (3F12): sc-8025. KNRK 全细胞裂解液中 Dvl-1 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214. 使用的检测试剂: m-IgGκ BP-CFL 790: sc-516181.
Dvl-1 抗体 (3F12): sc-8025. 对福尔马林固定, 石蜡包埋的人心肌组织进行免疫过氧化物酶染色, 显示心肌细胞的胞浆染色.

Dvl-1/Dishevelled 1/DVL1 抗体 (3F12): sc-8025

5.0(14)
寫評論提問

说明书
  • Dvl-1/Dishevelled 1/DVL1 抗体 3F12 是小鼠单克隆 IgG2a κ，Dvl-1/Dishevelled 1/DVL1抗体, 在81篇文献中引用，规格为200 µg/ml
  • 免疫mouse来源Dvl-1位于C-terminus 的651-695氨基酸
  • Dvl-1/Dishevelled 1/DVL1 抗体 (3F12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Dvl-1
  • 抗Dvl-1/Dishevelled 1/DVL1抗体（3F12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Dvl-1/Dishevelled 1/DVL1 (3F12): sc-8025的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 Dvl-1/Dishevelled 1/DVL1 抗体 (3F12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Dvl-1/Dishevelled 1/DVL1 抗体 (3F12) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Dishevelled 抗体 包括 Dvl, Dvl-1, Dvl-2和Dvl-3

Dvl-1抗体（3F12）是一种小鼠单克隆IgG2a kappa轻链抗体，用于通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类中的dishevelled segment polarity protein 1（Dvl-1）。Dvl-1是Wnt信号传导通路的关键组成部分，对调节胚胎发育、细胞增殖和分化至关重要。Dvl-1主要位于细胞质中并与细胞膜相关联，它充当着Wnt受体与下游效应器之间传递信号的关键中介，从而协调各种细胞过程。在结构上，Dvl-1由三个保守结构域组成，分别是DIX、PDZ和DEP结构域，它们有助于与Frizzled受体和Axin等关键蛋白相互作用，从而调节经典和非经典的Wnt信号通路。翻译后修饰，特别是磷酸化，在调节Dvl-1的活性和稳定性方面发挥着重要作用，这些修饰对于精确的信号转导和细胞功能至关重要。Dvl-1与多种蛋白相互作用，包括Frizzled家族成员和β-catenin破坏复合物的成分，这凸显了Dvl-1在维持细胞稳态和参与癌症和神经退行性疾病等病理条件中的重要性。抗Dvl-1抗体（3F12）是针对小鼠Dvl-1蛋白C端651-695氨基酸产生的。Dvl-1 (3F12)抗体有非结合型和多种结合型，包括辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物，为研究Wnt信号传导通路及其在发育生物学和疾病状态中的复杂机制的研究人员提供了多种应用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Dvl-1/Dishevelled 1/DVL1 抗体 (3F12) 参考文献:

  1. 果蝇节片极性基因 dishevelled 的小鼠同源物的分离和特征描述  |  Sussman, DJ., et al. 1994. Dev Biol. 166: 73-86. PMID: 7958461
  2. Dishevelled是果蝇褐飞虱信号通路的一个组成部分。  |  Krasnow, RE., et al. 1995. Development. 121: 4095-102. PMID: 8575310
  3. 在迪乔治综合征中，果蝇混乱片段-极性基因的人类同源序列被删除。  |  Pizzuti, A., et al. 1996. Am J Hum Genet. 58: 722-9. PMID: 8644734
  4. 果蝇散乱极性基因的两个人类同源物的 cDNA 表征和染色体图谱。  |  Pizzuti, A., et al. 1996. Hum Mol Genet. 5: 953-8. PMID: 8817329
  5. 小鼠 dishevelled-3 的分离和特征描述。  |  Tsang, M., et al. 1996. Dev Dyn. 207: 253-62. PMID: 8922524
  6. 一种frizzled同源物在脊椎动物Wnt信号通路中发挥作用  |  Yang-Snyder, J., et al. 1996. Curr Biol. 6: 1302-6. PMID: 8939578
  7. 人类鬅毛基因构成了一个含 DHR 的多基因家族。  |  Semënov, MV. and Snyder, M. 1997. Genomics. 42: 302-10. PMID: 9192851
  8. 在转染的哺乳动物细胞中，过表达小鼠 dishevelled-1 蛋白可抑制 GSK-3beta 介导的 tau 磷酸化。  |  Wagner, U., et al. 1997. FEBS Lett. 411: 369-72. PMID: 9271238

订购信息

产品名称产品编号规格价格数量收藏夹

Dvl-1/Dishevelled 1/DVL1 抗体 (3F12)

sc-8025
200 µg/ml
RMB2377.00

Dvl-1/Dishevelled 1/DVL1 (3F12): m-IgG Fc BP-HRP 套装

sc-525448
200 µg Ab; 10 µg BP
RMB2662.00

Dvl-1/Dishevelled 1/DVL1 (3F12): m-IgG2a BP-HRP 套装

sc-546261
200 µg Ab; 10 µg BP
RMB2662.00

Dvl-1/Dishevelled 1/DVL1 抗体 (3F12) AC

sc-8025 AC
500 µg/ml, 25% agarose
RMB3129.00

Dvl-1/Dishevelled 1/DVL1 抗体 (3F12) HRP

sc-8025 HRP
200 µg/ml
RMB2377.00

Dvl-1/Dishevelled 1/DVL1 抗体 (3F12) FITC

sc-8025 FITC
200 µg/ml
RMB2482.00

Dvl-1/Dishevelled 1/DVL1 抗体 (3F12) PE

sc-8025 PE
200 µg/ml
RMB2580.00

Dvl-1/Dishevelled 1/DVL1 抗体 (3F12) Alexa Fluor® 488

sc-8025 AF488
200 µg/ml
RMB2685.00

Dvl-1/Dishevelled 1/DVL1 抗体 (3F12) Alexa Fluor® 546

sc-8025 AF546
200 µg/ml
RMB2685.00

Dvl-1/Dishevelled 1/DVL1 抗体 (3F12) Alexa Fluor® 594

sc-8025 AF594
200 µg/ml
RMB2685.00

Dvl-1/Dishevelled 1/DVL1 抗体 (3F12) Alexa Fluor® 647

sc-8025 AF647
200 µg/ml
RMB2685.00

Dvl-1/Dishevelled 1/DVL1 抗体 (3F12) Alexa Fluor® 680

sc-8025 AF680
200 µg/ml
RMB2685.00

Dvl-1/Dishevelled 1/DVL1 抗体 (3F12) Alexa Fluor® 790

sc-8025 AF790
200 µg/ml
RMB2685.00

I find it is hard to detect WB band using Dvl-1 Antibody: sc-8025 (1:100 dilution) and m-IgGκ BP-HRP: sc-516102 (1:2000 dilution) as primary and secondary antibody. Should I use another secondary antibody? Any other recommendation for good results?

Asked by: SMJJ
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

没有出现85的条带。。。。只出现35左右的条带。。。你们有遇见这种情况吗

Asked by: 哈哈许松
感谢您的提问。关于此问题的详细细节，欢迎通过：电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”，联系我们的技术支持部门。 Thank you for your inquiry. For more detailed information about this question, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 15
Date published: 2017-09-11

各位，你们用这个抗体，有出现30多的条带出现吗？

Asked by: 哈哈许松
感谢您的提问。关于此问题的详细细节，欢迎通过：电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”，联系我们的技术支持部门。 Thank you for your inquiry. For more detailed information about this question, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 15
Date published: 2017-09-11

I am using Dvl-1 Antibody (3F12): sc-8025 to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenni
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 8
Date published: 2017-01-06
  • y_2025, m_9, d_7, h_4CST
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Rated 5 out of 5 by from work greatThe antibody was perfect. No background detected, very strong signal.
Date published: 2017-09-22
Rated 5 out of 5 by from strong signalThe Dvl-1 protein is highly expressed in the intestinal tract of Hirschsprung's disease tissue, and a strong signal is still obtained after dilution of 1:1000, this sample that can be used as a positive control,the specific band is at 85kda.
Date published: 2017-09-09
Rated 5 out of 5 by from Works greatExcellent antibody! One single (and strong) band at the right molecular weight
Date published: 2017-07-21
Rated 5 out of 5 by from Western blot is satisfiedNo background detected. there were target bands detected by sc-8025,very strong signal.
Date published: 2017-07-08
Rated 5 out of 5 by from 强大条带清楚，特异性强，单抗的效果还是不错的，荧光强度，稳定性都不错，我一直都用这个货号的抗体，继续关注
Date published: 2017-06-06
Rated 5 out of 5 by from good productionThe product quality is very good, and result of the band is very clear and strong even at a 1:800 dilution in western blot
Date published: 2017-05-01
Rated 5 out of 5 by from I am satisfied with this antibodyNot long ago ,I used this antibody for IHC(P).In my experiment,I diluted the antibody one hundred times.And I got wonderful results.
Date published: 2017-03-29
Rated 5 out of 5 by from Excellent IHC stainingGreat ihc staining;strong signal; also good for wb
Date published: 2017-01-04
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Dvl-1/Dishevelled 1/DVL1 抗体 (3F12) is rated 5.0 out of 5 by 14.
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