Date published: 2025-9-10
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DsRed Antibody (E-8) - Western Blotting - Image 290980
DsRed 抗体 (E-8): sc-390909. 纯化的重组 DsRed 的 Western 印迹分析.
DsRed Antibody (E-8) - Western Blotting - Image 290980
DsRed 抗体 (E-8): sc-390909. 纯化的重组 DsRed 的 Western 印迹分析.
DsRed 抗体 (E-8): sc-390909. 纯化的重组 DsRed 的 Western 印迹分析.

DsRed 抗体 (E-8): sc-390909

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寫評論提問

说明书
  • DsRed 抗体 E-8 是小鼠单克隆 IgG2b κ，DsRed抗体, 在44篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于Discosoma Sp.物种的DsRed的C-terminus的氨基酸178-223之间
  • DsRed 抗体 (E-8) 推荐用于 WB, IP, IF 和 ELISA，检测 proteins containing the DsRed tag
  • 抗DsRed抗体（E-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取DsRed (E-8): sc-390909的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是DsRed Antibody (E-8) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与DsRed Antibody (E-8) 打包提供（请参阅下面的订购信息）。

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描述
说明书与实验方案
研究信息

関連項目

附加DsRed抗体，包括DsRed2

DsRed抗体(E-8)是一种IgG2b κ小鼠单克隆DsRed抗体(也称为红色荧光蛋白(Red Fluorescent Protein, RFP) DsRed抗体)，通过WB, IP, IF和ELISA检测DsRed蛋白。DsRed 抗体 (E-8)既可以是非偶联的抗DsRed抗体形式，也可以是多种偶联的抗DsRed抗体形式，包括琼脂糖，HRP, PE, FITC和多个Alexa Fluor®偶联物。真核基因编码区在细菌、昆虫和哺乳动物宿主中表达的质粒载体是常用的载体;所述表达载体常用于编码由真核靶蛋白和为融合蛋白的荧光可视化而设计的专门区域组成的杂交融合蛋白。DsRed是从盘体珊瑚中克隆的与绿色荧光蛋白(GFP)同源的红色荧光蛋白。DsRed表现出对极端pH和光漂白的良好抗性，并且在成熟后引起从558 nm到583 nm的强红移。未成熟DsRed显示gfp样激发和发射最大值。通过GFP和DsRed光谱位移产生的对比允许一个强大的双重报告系统。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DsRed 抗体 (E-8) 参考文献:

  1. 珊瑚红色荧光蛋白 DsRed 的生物化学, 诱变和寡聚。  |  Baird, GS., et al. 2000. Proc Natl Acad Sci U S A. 97: 11984-9. PMID: 11050229
  2. 四聚体 GFP 同源物 DsRed 发出红色荧光的结构基础。  |  Wall, MA., et al. 2000. Nat Struct Biol. 7: 1133-8. PMID: 11101896
  3. 将红色荧光蛋白（DsRed）作为酿酒酵母的报告物。  |  Rodrigues, F., et al. 2001. J Bacteriol. 183: 3791-4. PMID: 11371544
  4. GFP 蓝宝石突变体的高效折叠和环状排列变体。  |  Zapata-Hommer, O. and Griesbeck, O. 2003. BMC Biotechnol. 3: 5. PMID: 12769828
  5. 带有未成熟和成熟发色团的 Discosoma 红色荧光蛋白的晶体结构：发色团成熟过程中肽键反式-顺式异构化和酰亚胺形成的联系。  |  Tubbs, JL., et al. 2005. Biochemistry. 44: 9833-40. PMID: 16026155
  6. 用于全细胞标记的非细胞毒性DsRed衍生物。  |  Strack, RL., et al. 2011. Methods Mol Biol. 699: 355-70. PMID: 21116992
  7. 基于DsRed荧光蛋白的敏感报告细胞系统，用于基因毒性和氧化应激评估。  |  Hendriks, G., et al. 2011. Mutat Res. 709-710: 49-59. PMID: 21382384
  8. 用于结核分枝杆菌的密码子优化DsRed荧光蛋白。  |  Carroll, P., et al. 2018. BMC Res Notes. 11: 685. PMID: 30285840
  9. 体外肿瘤细胞基因电转后GFP和DsRed荧光蛋白的表达。  |  Komel, T., et al. 2023. Bioelectrochemistry. 153: 108490. PMID: 37356264
  10. 在拟南芥双转化体的分生核和分化核中，EYFP-CENH3/H2B-DsRed的同步表达受到不同程度的影响。  |  Schubert, V., et al. 2023. Cytogenet Genome Res. 163: 74-80. PMID: 37552957
  11. 单体红色荧光蛋白DsRed的晶体结构数据。  |  Nam, KH. 2024. Data Brief. 57: 110905. PMID: 39376484
  12. 果蝇红色荧光蛋白报告器的增强子诱捕。  |  Akimoto, A., et al. 2005. Dev Dyn. 233: 993-7. PMID: 15906373

订购信息

产品名称产品编号规格价格数量收藏夹

DsRed 抗体 (E-8)

sc-390909
200 µg/ml
RMB2377.00

DsRed (E-8): m-IgGκ BP-HRP 套装

sc-523729
200 µg Ab, 40 µg BP
RMB2662.00

DsRed (E-8): m-IgG2b BP-HRP 套装

sc-549136
200 µg Ab; 10 µg BP
RMB2662.00

DsRed 抗体 (E-8) AC

sc-390909 AC
500 µg/ml, 25% agarose
RMB3129.00

DsRed 抗体 (E-8) HRP

sc-390909 HRP
200 µg/ml
RMB2377.00

DsRed 抗体 (E-8) FITC

sc-390909 FITC
200 µg/ml
RMB2482.00

DsRed 抗体 (E-8) PE

sc-390909 PE
200 µg/ml
RMB2580.00

DsRed 抗体 (E-8) Alexa Fluor® 488

sc-390909 AF488
200 µg/ml
RMB2685.00

DsRed 抗体 (E-8) Alexa Fluor® 546

sc-390909 AF546
200 µg/ml
RMB2685.00

DsRed 抗体 (E-8) Alexa Fluor® 594

sc-390909 AF594
200 µg/ml
RMB2685.00

DsRed 抗体 (E-8) Alexa Fluor® 647

sc-390909 AF647
200 µg/ml
RMB2685.00

DsRed 抗体 (E-8) Alexa Fluor® 680

sc-390909 AF680
200 µg/ml
RMB2685.00

DsRed 抗体 (E-8) Alexa Fluor® 790

sc-390909 AF790
200 µg/ml
RMB2685.00

DsRed (E-8) 中和勝肽

sc-390909 P
100 µg/0.5 ml
RMB511.00

Is this antibody recognise tdTomato as well?

Asked by: Michsh
Thank you for your question. We have not tested this antibody for its reactivity with dTomato. Due to the mismatches between the amino acid sequences of dTomato and DsRed, it is likely that the antibody will not be able to detect dTomato.
Answered by: Tech Support Europe
Date published: 2022-09-05

Can this antibody be used to detect other red fluorescent protein dTomato?

Asked by: Macc
Thank you for your question. We have not tested this antibody for its reactivity with dTomato. Due to the mismatches between the amino acid sequences of dTomato and DsRed, it is likely that the antibody will not be able to detect dTomato.
Answered by: Tech Support Europe
Date published: 2021-07-23

I want to use this antobody in Hydra which is strict to antibodies. Could you give a trial smaple?

Asked by: qiann
Thank you for your question. Please contact our Technical Service Department for more information on our sample promotion and to arrange for a 10 µg sample of DsRed (E-8): sc-390909.
Answered by: Tech Support Europe
Date published: 2021-03-22

Has this antibody been used with marine invertebrate? Thank you, Noa

Asked by: Rotemn
Thanks for your question. Yes, DsRed (E-8) Ab is recommended for detection of proteins containing the DsRed tag of Discosoma Sp. origin by WB, IP, IF and ELISA. Please contact technical service if you need any other information.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Has this antibody been used with drosophila? Thank you, Staci

Asked by: Staci
Thank you for the question. We have not tested this clone on Drosophila samples. However, we believe this should work well and we do warrant such application. If you prefer to test this clone on your samples prior to purchasing a full vial with our product warranty, please contact us by email to receive a FREE 10 µg sample of DsRed (E-8): sc-390909.
Answered by: Technical Support Europe
Date published: 2018-02-26

Does it cross reacts to GFP?

Asked by: Nkkushwaha
Thank you for your question. This antibody is specific for DsRed and should not cross-react with GFP. Please contact our Technical Service Department by phone, e-mail or live chat if you have any further questions. Alternatively, you can contact your local distributor: https://www.scbt.com/scbt/resources/distributors
Answered by: Tech Service
Date published: 2017-08-07

Can this antibody be used to detect other red fluorescent proteins such as RFP or mCherry?

Asked by: AbPolly
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about reactivity with related proteins.
Answered by: Technical Support 12
Date published: 2023-04-17
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Rated 4 out of 5 by from Detection of mRFP-fusion proteinsFacilitating the detection of mRFP fusion proteins, the DsRed antibody yielded well defined bands, ensuring the reliability of our experiments. Its precision in identifying DsRed-labeled proteins enhanced the robustness of our protein analyses.
Date published: 2024-01-04
Rated 1 out of 5 by from Does not work with DrosophilaStrong, non-specific signal in Drosophila larval and adult for both immunohistochemistry and western blotting. IHC 1:500 in 5% NGS+2% BSA in PBST block. WB: 1:100-1:5000 in 5% milk. No specific bands or IHC signal (not also present in negative controls) was detected.
Date published: 2019-10-23
Rated 5 out of 5 by from Great ABI used this antibody to track a viral injection of a mCherry-expressing AAV in the in the mouse brain and it worked perfectly. It was used in 1/200 final concentration on 4% PFA fixed tissue.
Date published: 2019-02-13
Rated 5 out of 5 by from Excellent!!!The unique antibody we know that works for ihc on mouse paraffin samples!!
Date published: 2018-05-12
Rated 5 out of 5 by from 抗体效果好，特异性强。这个抗体是我一个月前买的抗体，使用1:1000的稀释抗体，图中所见DsRed2蛋白条带很明显，抗体效果好，特异性强。接下来我想用它来做IP实验，相信能获得良好的效果。
Date published: 2018-05-03
Rated 1 out of 5 by from Didn't work :(Unfortunately antibody didn't work for mCherry either in Caenorhabditis expressing mCherry or wormScarlet. It also didn't work for yeast strain expressing mCherry. I used 1:100 dilution.
Date published: 2018-03-23
Rated 4 out of 5 by from Works well for overexpressed protein in 293A cellsWorks well for WB in 293A lysates using an overexpressed fusion protein. Reasonably low background. Used LI-COR secondary for detection.
Date published: 2018-03-10
Rated 1 out of 5 by from Weak signalI have been tried 1:1000, 1:500 and the signal is very very weak. Planning to go higher concentration but overall disappointed.
Date published: 2018-02-23
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DsRed 抗体 (E-8) is rated 3.8 out of 5 by 24.
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