Date published: 2025-9-8
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DSPP Antibody (LFMb-21) - Western Blotting - Image 370517
DSPP 抗体 (LFMb-21): sc-73632. LNCaP (A) 和 AT-3 (B) 全细胞裂解液中 DSPP 表达的 Western 印迹分析.
DSPP 抗体 (LFMb-21): sc-73632. LNCaP (A) 和 AT-3 (B) 全细胞裂解液中 DSPP 表达的 Western 印迹分析.

DSPP 抗体 (LFMb-21): sc-73632

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寫評論提問

说明书
  • DSPP 抗体 LFMb-21 是小鼠单克隆 IgG2b κ，DSPP抗体, 在116篇文献中引用，规格为200 µg/ml
  • 免疫human物种的DSPP的氨基酸487-502
  • DSPP 抗体 (LFMb-21) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat, human 和monkey 来源的 DSPP and DPP; 不会与 DSP产生交联反应
  • 抗DSPP抗体（LFMb-21）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取DSPP (LFMb-21): sc-73632的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2b BP-HRP 是 DSPP 抗体 (LFMb-21) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与DSPP 抗体 (LFMb-21) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

DSP 抗体

DSPP抗体（LFMb-21）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠、人类和猴源性DSPP蛋白。DSPP (LFMb-21) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。DSPP蛋白（即牙本质唾液磷蛋白）可被切割成两种功能性蛋白：牙本质唾液蛋白（DSP）和牙本质磷蛋白（DPP）。这两种蛋白是牙齿中牙本质细胞外基质的组成部分，其中DSP在牙本质生成过程中起着关键作用，而DPP则对钙的结合至关重要，钙的加入有助于牙本质基质胶原的初步矿化，并调节矿化晶体的大小和形状。DSPP的正常功能对于牙齿发育和完整性至关重要，因为DSPP基因突变可导致DFNA39/DGI1（表现为渐进性听力下降和牙本质发育不全）以及DGI2和DGI3（导致牙齿脆弱、变色）等疾病。了解DSPP及其衍生物在牙齿健康中的作用对于制定这些遗传疾病的疗法至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DSPP 抗体 (LFMb-21) 参考文献:

  1. 牙本质纤溶磷蛋白（DSPP）在小鼠组织中的特异性表达及其多态性。  |  Yuan, G., et al. 2009. Cell Biol Int. 33: 816-29. PMID: 19450697
  2. 两个家族中与确定的牙本质鞘磷蛋白突变相关的牙釉质畸形。  |  Wang, SK., et al. 2011. Eur J Oral Sci. 119 Suppl 1: 158-67. PMID: 22243242
  3. 一个中国 DGI-II 家族的 DSPP 基因突变鉴定及最新生物信息学分析。  |  Li, D., et al. 2012. Genomics. 99: 220-6. PMID: 22310900
  4. 不同类别的突变牙本质纤溶磷蛋白（DSPP）所导致的粗面内质网转运错误，通过夹带正常的牙本质纤溶磷蛋白，在牙本质发育不全症和牙本质发育不良症中都会产生显性负效应。  |  von Marschall, Z., et al. 2012. J Bone Miner Res. 27: 1309-21. PMID: 22392858
  5. DSPP 基因突变导致的遗传性牙病。  |  Maciejewska, I. and Chomik, E. 2012. J Dent. 40: 542-8. PMID: 22521702
  6. 牙本质矽磷蛋白和牙本质基质蛋白-1：矿化组织中两种高度磷酸化的蛋白质。  |  Suzuki, S., et al. 2012. Arch Oral Biol. 57: 1165-75. PMID: 22534175
  7. 牙本质唾液腺蛋白-磷脂酰蛋白的加工效率受到裂解位点侧翼和远处突变的影响。  |  Yang, RT., et al. 2013. J Biol Chem. 288: 6024-33. PMID: 23297400
  8. 导致牙本质发育不全症的 DSPP 基因突变及突变效应的特征。  |  Lee, SK., et al. 2013. Biomed Res Int. 2013: 948181. PMID: 23509818
  9. 牙本质糖蛋白结构域介导人类牙周韧带干细胞的增殖和分化。  |  Ozer, A., et al. 2013. PLoS One. 8: e81655. PMID: 24400037
  10. 磷脂的黏合剂和迁移效应受整合素结合基序侧翼肽的调节。  |  Suzuki, S., et al. 2014. PLoS One. 9: e112490. PMID: 25396425
  11. 猪牙髓和牙本质中的牙本质sialophosphoprotein衍生蛋白--基因表达和功能。  |  Yamamoto, R. and Yamakoshi, Y. 2016. J Oral Biosci. 58: 120-127. PMID: 32512680
  12. 牙本质唾液磷蛋白（DSPP）在颅面发育中的作用。  |  Figueredo, CA., et al. 2022. J Oral Biol Craniofac Res. 12: 673-678. PMID: 36062256

订购信息

产品名称产品编号规格价格数量收藏夹

DSPP 抗体 (LFMb-21)

sc-73632
200 µg/ml
RMB2377.00

DSPP (LFMb-21): m-IgG Fc BP-HRP 套装

sc-525702
200 µg Ab; 10 µg BP
RMB2662.00

DSPP (LFMb-21): m-IgG2b BP-HRP 套装

sc-549627
200 µg Ab; 10 µg BP
RMB2662.00

DSPP 抗体 (LFMb-21) AC

sc-73632 AC
500 µg/ml, 25% agarose
RMB3129.00

DSPP 抗体 (LFMb-21) HRP

sc-73632 HRP
200 µg/ml
RMB2377.00

DSPP 抗体 (LFMb-21) FITC

sc-73632 FITC
200 µg/ml
RMB2482.00

DSPP 抗体 (LFMb-21) PE

sc-73632 PE
200 µg/ml
RMB2580.00

DSPP 抗体 (LFMb-21) Alexa Fluor® 488

sc-73632 AF488
200 µg/ml
RMB2685.00

DSPP 抗体 (LFMb-21) Alexa Fluor® 546

sc-73632 AF546
200 µg/ml
RMB2685.00

DSPP 抗体 (LFMb-21) Alexa Fluor® 594

sc-73632 AF594
200 µg/ml
RMB2685.00

DSPP 抗体 (LFMb-21) Alexa Fluor® 647

sc-73632 AF647
200 µg/ml
RMB2685.00

DSPP 抗体 (LFMb-21) Alexa Fluor® 680

sc-73632 AF680
200 µg/ml
RMB2685.00

DSPP 抗体 (LFMb-21) Alexa Fluor® 790

sc-73632 AF790
200 µg/ml
RMB2685.00

Hello. Does this anti-DSPP antibody (LFMb-21) works well with rat tissue and IHC? And how much quantity is required for 80 samples? 

Asked by: Hira
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-07-22

Hi, the first antibody we ordered (Lot #I2908) was labeled as "DSP", while the second order (Lot #C1218) was labeled "DSPP". The epitope is located within DPP, so why was it originally called DSP? Was the epitope location switched?

Asked by: Jenjen
Thank you for your question. The epitope of this antibody has not changed. We changed the name to be a reference to the full length precursor protein, DSPP.
Answered by: Technical Support
Date published: 2018-12-01

Hi, I have a question regarding the labeling of this antibody as "DSPP". Its epitope is located within the DPP protein boundary, and when we perform the Western blot the detected band size is at 95-100 kDa. So shouldn't it be labeled "DPP" instead?

Asked by: Jenjen
Thank you for your question. This antibody is named "DSPP" for the full length precursor protein, dentin sialophosphoprotein. The epitope is mapping within the Dentin phosphoprotein or "DPP" so the antibody should be able to recognize both the precursor DSPP and mature DPP proteins.
Answered by: Technical Support
Date published: 2018-12-01

单克隆抗体，WB出现多条带，请问目的条带怎么判断，是130kDa附近的还是所有条带？

Asked by: DSPP
感谢您的提问。关于此问题的详细细节，欢迎通过：电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”，联系我们的技术支持部门。 Thank you for your inquiry. For more detailed information about this question, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 15
Date published: 2024-01-30

What kind of antigenic recovery is necessary for antigen detection in formalin-fixed and paraffin-embedded tissue?

Asked by: Maria Jose
Thank you for your question. We recommend using sodium citrate buffer (pH 6.0) with heat treatment as a preferred antigen retrieval method with this antibody, DSPP Antibody (LFMb-21): sc-73632. A link is provided below to our complete recommended protocol for IHC(P). https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-06

Can I use DSPP Antibody (LFMb-21): sc-73632 for immunohistochemical staining of mouse tissue?

Asked by: jenni
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-73632 PE), fluorescein (sc-73632 FITC), Alexa Fluor® 488 (sc-73632 AF488) or Alexa Fluor® 647 (sc-73632 AF647) Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 2
Date published: 2017-01-09
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Rated 5 out of 5 by from 效果稳定课题组一直使用该货号产品，WB效果稳定且条带清楚。用于免疫荧光实验的时候，也是定位特异，信号清晰，推荐推荐。
Date published: 2024-12-19
Rated 4 out of 5 by from Good signalThe antibody works very well in immunofluorescence (1:200) on mesenchymal stem cells fixed with 4% PFA.
Date published: 2020-01-08
Rated 5 out of 5 by from 条带很清楚~超好的~一个月前领到一个小样超级好用，wb结果与所给的图片一模一样。总之很好用的呢~一直用你家家抗体超好用的哟~
Date published: 2019-03-14
Rated 5 out of 5 by from beautiful bandsWe have used this product more than 3 years. We used this antibody to test DSPP expression in human DPS cells and the bands were beautiful.
Date published: 2018-09-27
Rated 5 out of 5 by from clear stripsWestern Blot results showed that the correct band size in diluted 1:500 work and saw excellent signals.
Date published: 2017-11-01
Rated 5 out of 5 by from 好用，能成功做出条带我同时用了三塔和proteint 家的DSPP抗体，可能由于湿转条件难度高，只有三塔的出结果了，protein家的一直没出
Date published: 2017-10-11
Rated 4 out of 5 by from Very goodAntibody sample, 1:200 dilution for immunostaining of osteogenic medium- induced human dental pulp cells, which showing cytoplasmic staining of cells.
Date published: 2017-08-18
Rated 5 out of 5 by from obtained tartget bandThis antiobdy is always appropriate to dilute at 1:500 to detect DSPP ,the target band is at the position 130kda.
Date published: 2017-07-01
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DSPP 抗体 (LFMb-21) is rated 4.8 out of 5 by 14.
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