Date published: 2025-9-8

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DREAM 抗体 (A-9): sc-166916

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说明书
  • DREAM 抗体 A-9 是小鼠单克隆 IgG2b κ,DREAM抗体, 在2篇文献中引用,规格为200 µg/ml
  • 免疫human物种的代表全长DREAM的氨基酸1-214
  • DREAM 抗体 (A-9) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 DREAM
  • 抗DREAM抗体(A-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取DREAM (A-9): sc-166916免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2b BP-HRP">m-IgG2b BP-HRP是DREAM Antibody (A-9) 适用于 WB 应用。 的首选辅助检测试剂,这些试剂现与DREAM Antibody (A-9) 打包提供(请参阅下面的订购信息)。

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関連項目

DREAM抗体(A-9)是一种IgG2b κ小鼠单克隆DREAM抗体(也称为DREAM抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的DREAM蛋白。DREAM抗体(A-9)有非偶联的抗DREAM抗体形式,以及多种偶联形式的抗DREAM抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。DREAM(用于DRE拮抗剂调节剂)是一种钙离子调节的转录抑制因子,特异性结合下游调控元件(DRE)。DRE是一种沉默基础转录的调控序列,定位于编码人催产素的基因启动子区域,催产素是一种参与记忆获取和疼痛的阿片肽。DREAM形成与DRE相互作用所需的功能性同源四聚体。这种关联受到钙的强烈影响,因为钙离子的增加直接抑制了DREAM的结合,从而阻断了DREAM的抑制活性。在脑、胸腺和甲状腺中检测到DREAM转录本,并且以核蛋白的形式表达。DREAM已被证明可以抑制含有DRE样基序的其他蛋白质的转录,包括编码AP-1转录因子c-Fos的基因,表明DREAM可能影响多种细胞基因。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DREAM 抗体 (A-9) 参考文献:

  1. 细胞信号无钙 DREAM  |  Mandel, G. and Goodman, RH. 1999. Nature. 398: 29-30. PMID: 10078526
  2. DREAM 是一种受 Ca2+ 调节的转录抑制因子。  |  Carrión, AM., et al. 1999. Nature. 398: 80-4. PMID: 10078534
  3. 神经母细胞瘤细胞中Ca(2+)依赖性原吗啡转录抑制是通过DREAM蛋白的活性以一种与激酶无关的方式实现的。  |  Campos, D., et al. 2003. Mol Cell Neurosci. 22: 135-45. PMID: 12676525
  4. 转录抑制因子DREAM与甲状腺转录因子-1相互作用,调节甲状腺球蛋白基因的表达。  |  Rivas, M., et al. 2004. J Biol Chem. 279: 33114-22. PMID: 15181011
  5. 转录抑制因子 DREAM 可调节 T 淋巴细胞增殖和细胞因子基因表达。  |  Savignac, M., et al. 2005. EMBO J. 24: 3555-64. PMID: 16177826
  6. DREAM(下游调节元件拮抗剂调节剂)有助于突触抑制和情境恐惧记忆。  |  Wu, LJ., et al. 2010. Mol Brain. 3: 3. PMID: 20205763
  7. 离子通量在控制 c-fos 表达中的作用  |  Morgan, JI. and Curran, T. Nature. 322: 552-5. PMID: 2426600
  8. 氢氘交换质谱揭示了钙结合特性和下游调节元件拮抗剂调节剂(DREAM)的变构调节。  |  Zhang, J., et al. 2017. Biochemistry. 56: 3523-3530. PMID: 28627884
  9. Pb2+与下游调节元件拮抗剂调节剂(DREAM)结合并调节其与结合伴侣的相互作用:神经元钙传感器与Pb2+神经毒性之间的联系。  |  Azam, S. and Miksovska, J. 2019. ACS Chem Neurosci. 10: 1263-1272. PMID: 30399317
  10. 阿片肽达吗啡介导海马苔藓纤维突触的异突触抑制并调节长期电位。  |  Weisskopf, MG., et al. 1993. Nature. 365: 188. PMID: 8103916
  11. 前脑啡肽和前脑啡肽在人脑中的信使 RNA 表达差异。  |  Hurd, YL. 1996. Neuroscience. 72: 767-83. PMID: 9157322
  12. 通过与基因内沉默元件的不同结合,蛋白激酶 A 依赖性地抑制人类原吗啡基因。  |  Carrión, AM., et al. 1998. Mol Cell Biol. 18: 6921-9. PMID: 9819380

订购信息

产品名称产品编号规格价格数量收藏夹

DREAM 抗体 (A-9)

sc-166916
200 µg/ml
RMB2377.00

DREAM (A-9): m-IgG Fc BP-HRP 套装

sc-525774
200 µg Ab; 10 µg BP
RMB2662.00

DREAM (A-9): m-IgG2b BP-HRP 套装

sc-549633
200 µg Ab; 10 µg BP
RMB2662.00

DREAM 抗体 (A-9) AC

sc-166916 AC
500 µg/ml, 25% agarose
RMB3129.00

DREAM 抗体 (A-9) HRP

sc-166916 HRP
200 µg/ml
RMB2377.00

DREAM 抗体 (A-9) FITC

sc-166916 FITC
200 µg/ml
RMB2482.00

DREAM 抗体 (A-9) PE

sc-166916 PE
200 µg/ml
RMB2580.00

DREAM 抗体 (A-9) Alexa Fluor® 488

sc-166916 AF488
200 µg/ml
RMB2685.00

DREAM 抗体 (A-9) Alexa Fluor® 546

sc-166916 AF546
200 µg/ml
RMB2685.00

DREAM 抗体 (A-9) Alexa Fluor® 594

sc-166916 AF594
200 µg/ml
RMB2685.00

DREAM 抗体 (A-9) Alexa Fluor® 647

sc-166916 AF647
200 µg/ml
RMB2685.00

DREAM 抗体 (A-9) Alexa Fluor® 680

sc-166916 AF680
200 µg/ml
RMB2685.00

DREAM 抗体 (A-9) Alexa Fluor® 790

sc-166916 AF790
200 µg/ml
RMB2685.00

I received the free sample of DREAM SC-166916 antibody and I got five different spots in Western Blot assay (using astrocytes samples from mice): 32 kDa (should be DREAM), 40 kDa, 48 kDa, 70 kDa and 80 kDa approximately. What could be those spots?

Asked by: Miroslava
Thank you for your product review. Please contact our Technical Service Department by phone, e-mail or live chat. We are happy to help you troubleshoot the antibody. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase.
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27

What is the recommended fixation method for immunofluorescence staining with DREAM (A-9): sc-166916 monoclonal antibody?

Asked by: Cweed
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24

we bought this product a few weeks ago. I'd like know that why this antibody detect both size of dream about 34kDa and 20kDa as ur datasheet mention. please let me know the reason that 20kDa size of dream

Asked by: rosalind
Thank you for your question. On our product datasheet for this mouse monoclonal antibody, DREAM (A-9): sc-166916, we list the predicted molecular weight for the DREAM protein as 32 kDa. It appears that your question is referring to the Western blot data published on the product datasheet. This data is not representative of detection of endogenous DREAM protein. The antibody was used to detect DREAM protein in a transfected whole cell lysate. The full-length DREAM protein was observed in the transfected lysate around 32 kDa. The lower molecular weight band has not been characterized, but it is presumed to be an incomplete translation product of the transfected cDNA.
Answered by: Technical Support
Date published: 2016-10-03
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Rated 1 out of 5 by from This antibody did not work at all.We tested this antibody in lysates of several cells expressing DREAM, but it did not work.
Date published: 2020-03-11
Rated 5 out of 5 by from Great monoclonal antibodyNice strong band observed with DREAM Antibody (A-9). IF stain was robust and specific
Date published: 2017-02-09
Rated 5 out of 5 by from All is okWe can see that DREAM has been shown to inhibit the transcription of proteins containing DRE-like motifs, including genes encoding the c-Fos protein for the AP-1 transcription factor, suggesting that DREAM may affect the genes of many cells.
Date published: 2017-01-24
Rated 5 out of 5 by from Good WB data with Mouse liverI bought this new antibody recently,got the good results,i used it with 1:1000,and the new antibody is very good for past and future
Date published: 2017-01-07
Rated 5 out of 5 by from Produced superb immunofluorescence cytoplasmicProduced superb immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-19
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DREAM 抗体 (A-9) is rated 4.3 out of 5 by 6.
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