Date published: 2025-9-8
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Dnmt3a Antibody (C-12) - Western Blotting - Image 376129
Dnmt3a 抗体 (C-12): sc-365769. P19 (A), F9 (B) 和 C6 (C) 全细胞裂解液中 Dnmt3a 表达的 Western 印迹分析.
Dnmt3a 抗体 (C-12): sc-365769. P19 (A), F9 (B) 和 C6 (C) 全细胞裂解液中 Dnmt3a 表达的 Western 印迹分析.

Dnmt3a 抗体 (C-12): sc-365769

4.3(12)
寫評論提問

说明书
  • Dnmt3a 抗体 C-12 是小鼠单克隆 IgG2b κ，Dnmt3a抗体, 在24篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Dnmt3a的N-terminus附近的氨基酸42-67之间
  • Dnmt3a 抗体 (C-12) 推荐用于 WB, IP 和 ELISA，检测mouse, rat 和human 来源的 Dnmt3a
  • 抗Dnmt3a抗体（C-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Dnmt3a (C-12): sc-365769的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是Dnmt3a Antibody (C-12) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Dnmt3a Antibody (C-12) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Dnmt抗体，包括Dnmt1, Dnmt2, Dnmt3a, Dnmt3b和Dnmt3L

Dnmt3a抗体（C-12）是一种IgG2b κ小鼠单克隆Dnmt3a抗体（也称为DNMT3a抗体或DNA甲基转移酶3A抗体），可通过WB、IP和ELISA检测小鼠、大鼠和人源的Dnmt3a蛋白。Dnmt3a抗体（C-12）既可以是非偶联抗Dnmt3a抗体形式，也可以是多种偶联形式的抗Dnmt3a抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。胞嘧啶5'位置的甲基化是目前已知的唯一一种哺乳动物基因组的自然共价修饰。DNA甲基化需要DNA 5-胞嘧啶甲基转移酶（Dnmt）蛋白的酶活性，这种酶能够催化甲基从S-腺苷甲硫氨酸转移到位于双核苷酸CpG基序中的胞嘧啶的5'位置，而这种甲基化会导致目标基因的转录抑制。Dnmt酶由独立的基因编码。Dnmt1是最丰富的，它优先甲基化半甲基化的DNA，并在发育过程中协调基因表达。其他哺乳动物Dnmt蛋白还包括Dnmt2和Dnmt3。Dnmt2缺乏Dnmt1的大N端调节结构域，在成年组织中的表达水平要低得多，并且可能参与新整合的逆转录病毒DNA的甲基化。Dnmt3a和Dnmt3b由两个不同的基因编码，但两者都在胚胎干细胞中大量表达，它们也参与DNA上CpG基序的甲基化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Dnmt3a 抗体 (C-12) 参考文献:

  1. DNA 甲基转移酶在早期发育过程中被积极地保留在细胞质中。  |  Cardoso, MC. and Leonhardt, H. 1999. J Cell Biol. 147: 25-32. PMID: 10508852
  2. 小鼠从头甲基转移酶 Dnmt3a 和 Dnmt3b 的体内活性。  |  Hsieh, CL. 1999. Mol Cell Biol. 19: 8211-8. PMID: 10567546
  3. DNA 甲基转移酶 Dnmt1 与组蛋白去乙酰化酶的活性有关。  |  Fuks, F., et al. 2000. Nat Genet. 24: 88-91. PMID: 10615135
  4. 人类 DNA 甲基转移酶（dnmt1）基因的转录调控。  |  Bigey, P., et al. 2000. Gene. 242: 407-18. PMID: 10721735
  5. 小鼠胚胎植入不需要子宫Dnmt3a。  |  Li, N., et al. 2020. Curr Mol Med. 20: 633-642. PMID: 32096741
  6. 小鼠造血细胞中的体细胞Dnmt3a失活会导致缓慢、典型的DNA甲基化丢失。  |  Smith, AM., et al. 2022. iScience. 25: 104004. PMID: 35313694
  7. DNMT3A R882H突变通过NRF2/NQO1轴调节糖酵解，促进急性白血病细胞的存活。  |  Chu, X., et al. 2023. Cell Signal. 105: 110626. PMID: 36758683
  8. Dnmt3a的缺失会通过PI3Kinase通路破坏造血平衡和髓细胞偏转。  |  Palam, LR., et al. 2023. JCI Insight. 8: PMID: 36976647
  9. [急性髓细胞白血病中DNMT3A基因表达的研究进展]。  |  Zhou, J., et al. 2024. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 41: 1010-1015. PMID: 39097288
  10. 小鼠细胞和组织中的 DNA（胞嘧啶-5）-甲基转移酶。使用基于机制的探针进行研究。  |  Yoder, JA., et al. 1997. J Mol Biol. 270: 385-95. PMID: 9237905
  11. 胚胎干细胞中病毒 DNA 的从头甲基化和维持甲基化不需要 Dnmt2。  |  Okano, M., et al. 1998. Nucleic Acids Res. 26: 2536-40. PMID: 9592134
  12. 胞嘧啶甲基化与哺乳动物的发育  |  Walsh, CP. and Bestor, TH. 1999. Genes Dev. 13: 26-34. PMID: 9887097

订购信息

产品名称产品编号规格价格数量收藏夹

Dnmt3a 抗体 (C-12)

sc-365769
200 µg/ml
RMB2377.00

Dnmt3a (C-12): m-IgG Fc BP-HRP 套装

sc-529460
200 µg Ab; 10 µg BP
RMB2662.00

Dnmt3a (C-12): m-IgGκ BP-HRP 套装

sc-522358
200 µg Ab, 40 µg BP
RMB2662.00

Dnmt3a (C-12): m-IgG2b BP-HRP 套装

sc-550114
200 µg Ab; 10 µg BP
RMB2662.00

Dnmt3a 抗体 (C-12) AC

sc-365769 AC
500 µg/ml, 25% agarose
RMB3129.00

Dnmt3a 抗体 (C-12) HRP

sc-365769 HRP
200 µg/ml
RMB2377.00

Dnmt3a 抗体 (C-12) FITC

sc-365769 FITC
200 µg/ml
RMB2482.00

Dnmt3a 抗体 (C-12) PE

sc-365769 PE
200 µg/ml
RMB2580.00

Dnmt3a 抗体 (C-12) Alexa Fluor® 488

sc-365769 AF488
200 µg/ml
RMB2685.00

Dnmt3a 抗体 (C-12) Alexa Fluor® 546

sc-365769 AF546
200 µg/ml
RMB2685.00

Dnmt3a 抗体 (C-12) Alexa Fluor® 594

sc-365769 AF594
200 µg/ml
RMB2685.00

Dnmt3a 抗体 (C-12) Alexa Fluor® 647

sc-365769 AF647
200 µg/ml
RMB2685.00

Dnmt3a 抗体 (C-12) Alexa Fluor® 680

sc-365769 AF680
200 µg/ml
RMB2685.00

Dnmt3a 抗体 (C-12) Alexa Fluor® 790

sc-365769 AF790
200 µg/ml
RMB2685.00

Dnmt3a (C-12) 中和勝肽

sc-365769 P
100 µg/0.5 ml
RMB511.00

Hi, I want to use this antibody for WB in tissue lysate from mouse hippocampus. Should I need to do nuclear cytoplasmic fraction? Or I can use RIPA buffer and use whole tisssue lysate?

Asked by: Debpali
Thank you for your question. Unfortunately we do not have any data on whether cytoplasmic fractions would produce significantly better results than simply using whole tissue lysates. We would be happy to hear how your experiments turn out. If you post a short review about your results here on the product page we will send you a CruzCredit coupon code that you can use for a discount on your next order.
Answered by: Tech Support Europe
Date published: 2021-06-25

Can I use the anti-Dnmt3a-AF647 antibody ( sc-365769 AF647) for flow cytometry? If so, do you have a recommended starting point for the concentration to be used?

Asked by: Akp1
Thank you for your question. sc-365769 AF647 has not been tested for flow cytometry in house, so we do not warranty it for that use, however it may work perfectly well. We recommend using ~1µg per million cells. Here is a link to our recommended protocol: https://www.scbt.com/scbt/resources/protocols/flow-cytometry If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-06-26

I want to run a Western blot using this Dnmt3a antibody using NIH/3T3 cell lysate. How much should I load on my gel?

Asked by: TinTin
We recommend loading ~50µg of cell lysate on your gel. You can find more about the recommended conditions in our Western blotting protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Service 11
Date published: 2017-01-19
  • y_2025, m_9, d_7, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_zh_CN, sid_365769, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 109ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Work well!1:200 diluted for wb of rat protein,the band is strong
Date published: 2018-03-19
Rated 4 out of 5 by from The band is clear,but there are other bands1:200 diluted for wb of rat pretein,the band is clear,but there are other bands
Date published: 2018-02-13
Rated 5 out of 5 by from 抗体很好用我在人源正常细胞、人源以及鼠源肿瘤细胞中进行Western blot检测，皆能很好的杂出清晰唯一的条带，实验效果很棒！
Date published: 2017-11-27
Rated 2 out of 5 by from Scarsa specificitàIn figura è mostrato il WB contro DNMT3A su lisato della linea cellulare di Neuroblastoma SH-SY5Y
Date published: 2017-11-20
Rated 4 out of 5 by from a good antibody for WBThis antibody recognizes a band at the predicted M.W on the WB (1:500 dilution, 40 ug HeLa lysate). However, it does not appear to stain the nuclei in formaldehyde-fixed HeLa cells
Date published: 2017-07-30
Rated 5 out of 5 by from SuperbGreat product for western blot studies; also strong IHC staining results
Date published: 2017-02-03
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of Dnmt3a expression in NIH/3T3 and Jurkat whole cell lysates.
Date published: 2017-01-12
Rated 4 out of 5 by from Monoclonal antibodiesI tried DNMT3A mAb (C12) and I am convinced with that..
Date published: 2016-12-19
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_zh_CN, sid_365769, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 17ms
  • REVIEWS, PRODUCT
Dnmt3a 抗体 (C-12) is rated 4.3 out of 5 by 12.
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_zh_CN, sid_365769, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 119ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。