Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Dnmt1 Antibody (H-12) - Western Blotting - Image 353162
Dnmt1 抗体 (H-12): sc-271729. Jurkat (A), Raji (B) 和 Ramos (C) 全细胞裂解液中 Dnmt1 表达的 Western 印迹分析.
Dnmt1 抗体 (H-12): sc-271729. Jurkat (A), Raji (B) 和 Ramos (C) 全细胞裂解液中 Dnmt1 表达的 Western 印迹分析.

Dnmt1 抗体 (H-12): sc-271729

4.4(21)
寫評論提問

说明书
  • Dnmt1 抗体 H-12 是小鼠单克隆 IgG1 κ，Dnmt1抗体, 在72篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1317-1616位于human物种的Dnmt1的C-terminus附近
  • Dnmt1 抗体 (H-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Dnmt1
  • 抗Dnmt1抗体（H-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Dnmt1 (H-12): sc-271729的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Dnmt1 抗体 (H-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Dnmt1 抗体 (H-12) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Dnmt 抗体 包括 Dnmt1, Dnmt2, Dnmt3a, Dnmt3b和Dnmt3L

Dnmt1抗体（H-12）是一种IgG1 κ小鼠单克隆Dnmt1抗体（也称为DNMT1抗体或DNA甲基转移酶1抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人源的Dnmt1蛋白。Dnmt1抗体（H-12）既可以是非偶联的抗Dnmt1抗体形式，也可以是多种偶联形式的抗Dnmt1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。胞嘧啶5'位置的甲基化是哺乳动物基因组中唯一已知的自然发生的共价修饰。DNA甲基化需要DNA 5-胞嘧啶甲基转移酶（Dnmt）蛋白的酶活性，该酶催化甲基从S-腺苷甲硫氨酸转移到位于双核苷酸CpG基序中的胞嘧啶的5'位置，这种甲基化导致目标基因的转录抑制。Dnmt酶由独立基因编码。Dnmt1是最丰富的，它优先甲基化半甲基化的DNA并在发育过程中协调基因表达。其他哺乳动物Dnmt蛋白包括Dnmt2和Dnmt3。Dnmt2缺乏Dnmt1的大N端调节域，在成人组织中的表达水平要低得多，并且可能参与新整合的逆转录病毒DNA的甲基化。Dnmt3a和Dnmt3b由两个不同的基因编码，但两者都在胚胎干细胞中大量表达，它们也在DNA上甲基化CpG基序。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Dnmt1 抗体 (H-12) 参考文献:

  1. DNA 甲基转移酶在早期发育过程中被积极地保留在细胞质中。  |  Cardoso, MC. and Leonhardt, H. 1999. J Cell Biol. 147: 25-32. PMID: 10508852
  2. 小鼠从头甲基转移酶 Dnmt3a 和 Dnmt3b 的体内活性。  |  Hsieh, CL. 1999. Mol Cell Biol. 19: 8211-8. PMID: 10567546
  3. DNA 甲基转移酶 Dnmt1 与组蛋白去乙酰化酶的活性有关。  |  Fuks, F., et al. 2000. Nat Genet. 24: 88-91. PMID: 10615135
  4. 人类 DNA 甲基转移酶（dnmt1）基因的转录调控。  |  Bigey, P., et al. 2000. Gene. 242: 407-18. PMID: 10721735
  5. 突变诱导的 DNMT1 分裂驱动神经退行性疾病。  |  Wang, W., et al. 2021. Sci Adv. 7: eabe8511. PMID: 34516921
  6. DNMT1通过诱导MEG3的过度甲基化作用促进乳腺癌的生长。  |  Zhu, X., et al. 2022. Cancer Cell Int. 22: 56. PMID: 35109842
  7. DNMT1/miR-130a/ZEB1 调控通路影响脂多糖诱导的脓毒症的炎症反应。  |  Ding, J., et al. 2022. DNA Cell Biol. 41: 479-486. PMID: 35486848
  8. GSK-3484862在细胞中靶向DNMT1降解。  |  Chen, Q., et al. 2023. NAR Cancer. 5: zcad022. PMID: 37206360
  9. 对疾病中 DNMT1 和程序性细胞死亡的见解。  |  Yan, L., et al. 2023. Biomed Pharmacother. 168: 115753. PMID: 37871559
  10. 小鼠细胞和组织中的 DNA（胞嘧啶-5）-甲基转移酶。使用基于机制的探针进行研究。  |  Yoder, JA., et al. 1997. J Mol Biol. 270: 385-95. PMID: 9237905
  11. 胚胎干细胞中病毒 DNA 的从头甲基化和维持甲基化不需要 Dnmt2。  |  Okano, M., et al. 1998. Nucleic Acids Res. 26: 2536-40. PMID: 9592134
  12. 胞嘧啶甲基化与哺乳动物的发育  |  Walsh, CP. and Bestor, TH. 1999. Genes Dev. 13: 26-34. PMID: 9887097

订购信息

产品名称产品编号规格价格数量收藏夹

Dnmt1 抗体 (H-12)

sc-271729
200 µg/ml
RMB2377.00

Dnmt1 (H-12): m-IgG Fc BP-HRP 套装

sc-527283
200 µg Ab; 10 µg BP
RMB2662.00

Dnmt1 (H-12): m-IgG1 BP-HRP 套装

sc-532656
200 µg Ab; 20 µg BP
RMB2662.00

Dnmt1 抗体 (H-12) AC

sc-271729 AC
500 µg/ml, 25% agarose
RMB3129.00

Dnmt1 抗体 (H-12) HRP

sc-271729 HRP
200 µg/ml
RMB2377.00

Dnmt1 抗体 (H-12) FITC

sc-271729 FITC
200 µg/ml
RMB2482.00

Dnmt1 抗体 (H-12) PE

sc-271729 PE
200 µg/ml
RMB2580.00

Dnmt1 抗体 (H-12) Alexa Fluor® 488

sc-271729 AF488
200 µg/ml
RMB2685.00

Dnmt1 抗体 (H-12) Alexa Fluor® 546

sc-271729 AF546
200 µg/ml
RMB2685.00

Dnmt1 抗体 (H-12) Alexa Fluor® 594

sc-271729 AF594
200 µg/ml
RMB2685.00

Dnmt1 抗体 (H-12) Alexa Fluor® 647

sc-271729 AF647
200 µg/ml
RMB2685.00

Dnmt1 抗体 (H-12) Alexa Fluor® 680

sc-271729 AF680
200 µg/ml
RMB2685.00

Dnmt1 抗体 (H-12) Alexa Fluor® 790

sc-271729 AF790
200 µg/ml
RMB2685.00

我想购买在斑马鱼体内工作的dnmt1抗体，已有文章报道Dnmt1 抗体 (H-300): sc-20701能在斑马鱼中工作，但是sc-20701的替代品Dnmt1 抗体 (H-12): sc-271729 是否也在斑马鱼中工作呢？虽然这两个抗体的抗原表位是一样的，能够寄送一个小样验证一下？

Asked by: 西大建波
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

I would like to test antibody for DNA methyltransferase. I am going to buy Dnmt3b (G-9): sc-376043 from your company. Could I get a free 10 µg sample of Dnmt1 (H-12): sc-271729 & Dnmt3a (C-12): sc-365769 ? Thank you so much!

Asked by: snuha
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-05-21

I need an antibody to perform ChIP assay but since the DNMT1 antibody H-300 has been discontinued, I was wondering if the H-12 can also be used for ChIP although this application is not mentioned in the description

Asked by: JeanneD
Thank you for your question. Unfortunately, this mouse monoclonal, Dnmt1 Antibody (H-12): sc-271729, has not yet been confirmed to work in ChIP applications. For a limited time, we are offering a free 10 µg sample of the antibody. Please contact our Technical Service Department if you would like to request a free sample to test in your intended application. Please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2017-03-21

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Germaine
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-09
  • y_2025, m_9, d_7, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_zh_CN, sid_271729, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 104ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from No bandsDnmt1 (H-12) – sc-271729- free sample WB: No detectable signal at tested concentrations (up to 1:100) in MCF7, BT549, or U87 cells.
Date published: 2025-06-16
Rated 3 out of 5 by from funziona beninosi vede un segnale ma solo con elevata esposizione
Date published: 2019-02-23
Rated 3 out of 5 by from Not suitable for rat protein1:100 diluted for wb of rat heart protein ,the band is weak
Date published: 2018-03-19
Rated 4 out of 5 by from Good for wb1:100 diluted for wb of rat protein ,the band is clear,but a little weak
Date published: 2018-02-13
Rated 5 out of 5 by from We Got the Specific StainingWe bought Dnmt1 antibody a few months ago and tested human and mouse cells, they were very satisfied with the performance of the antibodies.
Date published: 2017-10-28
Rated 4 out of 5 by from not very goodIt's not good to do western blot, I can not get band.
Date published: 2017-08-19
Rated 5 out of 5 by from a relatively good antibody for WBThis antibody generates a good signal at the predicted M.W on the WB (1:500 dilution, 40 ug HeLa lysate).
Date published: 2017-07-30
Rated 5 out of 5 by from Very good antibody for WBGives a sharp band at the expected size. Used in a blot with 100K HCT116 cells. Used 1:1,000 in 5% milk.
Date published: 2017-07-12
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_22
  • loc_zh_CN, sid_271729, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 18ms
  • REVIEWS, PRODUCT
Dnmt1 抗体 (H-12) is rated 4.3 out of 5 by 22.
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_22
  • loc_zh_CN, sid_271729, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 103ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。