Date published: 2025-9-7

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DNA pol α 抗体 (F-6): sc-365040

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说明书
  • DNA pol α抗体(F-6)是小鼠单克隆IgG2b (kappa轻链),规格为200 µg/ml
  • 特异性抗原位于human物种的DNA pol α的N-terminus附近的氨基酸3-31之间
  • 推荐用于 mouse, rat 和 human 来源的DNA pol α WB, IP, IF 和 ELISA检测
  • 2b BP-HRP">m-IgG2b BP-HRP是DNA pol α Antibody (F-6) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与DNA pol α Antibody (F-6) 打包提供(请参阅下面的订购信息)。

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    関連項目

    DNA的复制、重组和修复是维持基因组稳定性所必需的过程,这些过程需要核酸外切酶的参与。在DNA复制过程中,这些酶参与冈崎片段的加工;而在DNA修复过程中,它们的功能是切除受损的DNA片段并纠正重组错配。这些核酸外切酶包括DNA聚合酶家族。DNA聚合酶α、β、δ和ε参与DNA的复制和修复。DNA聚合酶δ和DNA聚合酶ε是多亚基酶,其中DNA聚合酶δ由两个亚基组成:p125,它与滑动DNA夹蛋白PCNA相互作用;另一个是p50。核编码的DNA聚合酶γ是线粒体DNA复制所需的唯一DNA聚合酶。DNA聚合酶ζ在各种组织中普遍表达,并介导损伤诱导突变的细胞机制。DNA聚合酶θ是一种与ATP结合的DNA聚合酶-解旋酶,并参与链间交联的修复。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    DNA pol α 抗体 (F-6) 参考文献:

    1. 在长时间刺激人类淋巴细胞期间,DNA聚合酶-α和-β的变化。  |  Bertazzoni, U., et al. 1976. Proc Natl Acad Sci U S A. 73: 785-9. PMID: 1062788
    2. 离心加速产生的超重力对DNA聚合酶-α的刺激。  |  Takemura, M. and Yoshida, S. 2001. Biochem Biophys Res Commun. 289: 345-9. PMID: 11716478
    3. 紫外线损伤的HeLa细胞中,DNA聚合酶α抑制剂抑制DNA切除。  |  Joe, CO., et al. 1990. Toxicol Lett. 52: 253-9. PMID: 2117783
    4. DNA聚合酶-α的swi7H4突变等位基因在DNA损伤检查点反应中的作用。  |  Khan, S. and Ahmed, S. 2015. PLoS One. 10: e0124063. PMID: 25822347
    5. 抗肿瘤毒素CD437是DNA聚合酶α的直接抑制剂。  |  Han, T., et al. 2016. Nat Chem Biol. 12: 511-5. PMID: 27182663
    6. DNA聚合酶α的活性不受蛋白激酶或碱性磷酸酶的影响。  |  Prussak, CE. and Tseng, BY. 1989. Biochem Biophys Res Commun. 159: 1397-403. PMID: 2930569
    7. DNA聚合酶α的活性与人类细胞DNA合成减少和年龄增长有关。  |  Busbee, D., et al. 1987. J Natl Cancer Inst. 79: 1231-9. PMID: 3480375
    8. 针对人类DNA聚合酶α的单克隆抗体可抑制渗透性人类细胞的DNA复制。  |  Miller, MR., et al. 1985. J Biol Chem. 260: 134-8. PMID: 3917431
    9. DNA聚合酶α的酶学特性。来自人类KB细胞的均相酶的基本催化特性、过程性和间隙利用率。  |  Fisher, PA., et al. 1979. J Biol Chem. 254: 6128-37. PMID: 447699
    10. 线粒体DNA复制不涉及DNA聚合酶α。  |  Zimmermann, W., et al. 1980. J Biol Chem. 255: 11847-52. PMID: 6777385

    订购信息

    产品名称产品编号规格价格数量收藏夹

    DNA pol α 抗体 (F-6)

    sc-365040
    200 µg/ml
    RMB2377.00

    DNA pol α (F-6): m-IgG2b BP-HRP 套装

    sc-548548
    200 µg Ab; 10 µg BP
    RMB2662.00

    DNA pol α (F-6) 中和勝肽

    sc-365040 P
    100 µg/0.5 ml
    RMB511.00

    What application is the blocking peptide sc-365040 P appropriate for?

    Asked by: DefinitelyNotMatt
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-02-27
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    Rated 4 out of 5 by from Western blot was good when we ran this againstWestern blot was good when we ran this against human cell samples.
    Date published: 2015-10-20
    Rated 5 out of 5 by from Produced great immunofluorescence nuclearProduced great immunofluorescence nuclear, perinuclear and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
    Date published: 2015-04-03
    Rated 5 out of 5 by from Great Western Blot data of DNA pol expressionGreat Western Blot data of DNA pol expression in HeLa and Jurkat nuclear extracts. -SCBT QC
    Date published: 2014-09-01
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    DNA pol α 抗体 (F-6) is rated 4.7 out of 5 by 3.
    • y_2025, m_8, d_30, h_9
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