Date published: 2025-9-7
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DNA-PK<sub>CS</sub> Antibody (G-4) - Western Blotting - Image 299
DNA-PKCS (G-4): sc-5282. 在 MOLT-4 (A), HeLa (B), K-562 (C) 和 CCRF-CEM (D) 全细胞裂解液中 DNA-PKCS 表达的 Western 印迹分析.
DNA-PKCS (G-4): sc-5282. 在 MOLT-4 (A), HeLa (B), K-562 (C) 和 CCRF-CEM (D) 全细胞裂解液中 DNA-PKCS 表达的 Western 印迹分析.

DNA-PKCS 抗体 (G-4): sc-5282

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寫評論提問

说明书
  • DNA-PKCS 抗体 G-4 是小鼠单克隆 IgG1 κ，DNA-PKCS抗体, 在79篇文献中引用，规格为200 µg/ml
  • 免疫human物种的DNA-PKCS (DNA-dependent protein kinase catalytic subunit)的氨基酸2965-4127
  • DNA-PKCS 抗体 (G-4) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 DNA-PKcs
  • 抗DNA-PKCS抗体（G-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取DNA-PKCS (G-4): sc-5282的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是DNA-PKCS Antibody (G-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与DNA-PKCS Antibody (G-4) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

DNA-PKCS抗体（G-4）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹、免疫沉淀、免疫荧光和酶联免疫吸附等方法检测人类DNA-PKCS蛋白。DNA-PKCS (G-4) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。DNA-PKCS，也称为DNA依赖性蛋白激酶催化亚基，在DNA双链断裂（DSBs）修复中起着至关重要的作用，对于V(D)J重组至关重要，而V(D)J重组对于免疫多样性的发展至关重要。DNA-PKCS位于细胞核中，与Ku蛋白形成异源三聚体复合物，负责识别和结合DSBs，从而促进修复过程。DNA-PKCS的正常运作至关重要，因为缺陷会导致严重后果，包括对电离辐射的敏感性增加和DNA修复机制受损，这些与各种遗传疾病和癌症有关。DNA-PKCS单克隆抗体（G-4）使研究人员能够深入了解DNA修复机制以及DNA-PKCS在细胞对DNA损伤的反应中的意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DNA-PKCS 抗体 (G-4) 参考文献:

  1. 香兰素衍生物 6-溴-5-羟基-4-甲氧基苯甲醛在 HepG2 细胞中诱导细胞凋亡和自噬，同时 DNA-PKcs 被裂解，c-Myc 癌细胞蛋白被迅速破坏。  |  Yan, YQ., et al. 2007. Cancer Lett. 252: 280-9. PMID: 17316978
  2. 在缺乏 NHEJ 核心因子 Ku70/80, DNA-PKcs 和 XRCC4-LigIV 的情况下，非同源末端连接的致突变潜力。  |  Kuhfittig-Kulle, S., et al. 2007. Mutagenesis. 22: 217-33. PMID: 17347130
  3. [急性白血病患儿骨髓细胞中 PTEN mRNA, hTERT 和 DNA-PKcs 的表达]。  |  Chai, YH., et al. 2007. Zhonghua Er Ke Za Zhi. 45: 74-5. PMID: 17349160
  4. DNA-PKcs在低或高LET辐照后的旁观者效应中的作用  |  Kanasugi, Y., et al. 2007. Int J Radiat Biol. 83: 73-80. PMID: 17357428
  5. DNA-PKcs 可促进分叉逆转和化疗抗性。  |  Dibitetto, D., et al. 2022. Mol Cell. 82: 3932-3942.e6. PMID: 36130596
  6. DNA-PKcs通过引发线粒体功能障碍来促进败血症引起的多器官衰竭。  |  Zou, R., et al. 2022. J Adv Res. 41: 39-48. PMID: 36328752
  7. TBC1D15缺陷通过抑制DNA-PKcs胞质滞留和DNA损伤来保护多柔比星的心脏毒性。  |  Yu, W., et al. 2023. Acta Pharm Sin B. 13: 4823-4839. PMID: 38045047
  8. 什么情况下脂质激酶不是脂质激酶？当它是一种蛋白激酶时。  |  Hunter, T. 1995. Cell. 83: 1-4. PMID: 7553860
  9. PIK 相关激酶：DNA 修复, 重组和细胞周期检查点。  |  Keith, CT. and Schreiber, SL. 1995. Science. 270: 50-1. PMID: 7569949
  10. DNA 依赖性蛋白激酶催化亚基：磷脂酰肌醇 3-激酶和共济失调毛细血管扩张症基因产物的亲缘关系。  |  Hartley, KO., et al. 1995. Cell. 82: 849-56. PMID: 7671312
  11. AT基因的发现引发了生物医学研究的繁荣。  |  Nowak, R. 1995. Science. 268: 1700-1. PMID: 7792589
  12. 单个共济失调毛细血管扩张症基因的产物与 PI-3 激酶相似。  |  Savitsky, K., et al. 1995. Science. 268: 1749-53. PMID: 7792600

订购信息

产品名称产品编号规格价格数量收藏夹

DNA-PKCS 抗体 (G-4)

sc-5282
200 µg/ml
RMB2377.00

DNA-PKCS (G-4): m-IgG Fc BP-HRP 套装

sc-528143
200 µg Ab; 10 µg BP
RMB2662.00

DNA-PKCS (G-4): m-IgGκ BP-HRP 套装

sc-520449
200 µg Ab, 40 µg BP
RMB2662.00

DNA-PKCS (G-4): m-IgG1 BP-HRP 套装

sc-542786
200 µg Ab; 20 µg BP
RMB2662.00

DNA-PKCS 抗体 (G-4) AC

sc-5282 AC
500 µg/ml, 25% agarose
RMB3129.00

DNA-PKCS 抗体 (G-4) HRP

sc-5282 HRP
200 µg/ml
RMB2377.00

DNA-PKCS 抗体 (G-4) FITC

sc-5282 FITC
200 µg/ml
RMB2482.00

DNA-PKCS 抗体 (G-4) PE

sc-5282 PE
200 µg/ml
RMB2580.00

DNA-PKCS 抗体 (G-4) Alexa Fluor® 488

sc-5282 AF488
200 µg/ml
RMB2685.00

DNA-PKCS 抗体 (G-4) Alexa Fluor® 546

sc-5282 AF546
200 µg/ml
RMB2685.00

DNA-PKCS 抗体 (G-4) Alexa Fluor® 594

sc-5282 AF594
200 µg/ml
RMB2685.00

DNA-PKCS 抗体 (G-4) Alexa Fluor® 647

sc-5282 AF647
200 µg/ml
RMB2685.00

DNA-PKCS 抗体 (G-4) Alexa Fluor® 680

sc-5282 AF680
200 µg/ml
RMB2685.00

DNA-PKCS 抗体 (G-4) Alexa Fluor® 790

sc-5282 AF790
200 µg/ml
RMB2685.00

Can not find any information about where the epitope recognition this antibody is, eg. amino residues 2000-2100

Asked by: cHARLES
Thank you for your question.The antigenic epitope of this antibody is: 2965-4127 amino acids of anti-human DNA-PKCS.
Answered by: Jiajun Z
Date published: 2024-03-11

What is the total volume provided of DNA-PKCS (G-4): sc-5282 monoclonal antibody?

Asked by: Anna99
Each vial contains 200 &micro;g IgG1 kappa light chain in 1.0 ml of PBS with &lt; 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support 15
Date published: 2017-01-19
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Rated 5 out of 5 by from Great antibodyWorks very well by western blot (1:1000 dilution); I saw a clear band at the expected molecular weight
Date published: 2022-02-16
Rated 5 out of 5 by from This antibody works for Western blotWe acquired this antibody approximately 7 months ago and we performed a western blot on whole cell lysates of HeLa cells. We used a 1 to 1000 dilution and we can see clear bands with a 30 second exposure. It works.
Date published: 2018-06-21
Rated 5 out of 5 by from Great AntibodyGot a trial sample from Santa Cruz. The antibody is great with nice clean band in SH-SY5Y cells. Used at 1:1000 dilution.
Date published: 2018-06-20
Rated 5 out of 5 by from Great!I bought this two weeks ago. I tested it for mice DNA-PKcs by WB, it works very well.
Date published: 2017-08-20
Rated 5 out of 5 by from Superb productGreat western with strong signal, low background; nice IF staining
Date published: 2017-02-02
Rated 5 out of 5 by from Works Great!This antibody works great in western blot analysis of DNA-PKCS expression in MOLT-4, HeLa, K-562 and CCRF-CEM whole cell lysates.
Date published: 2017-01-12
Rated 4 out of 5 by from Various publishable data in KA using humanVarious publishable data in KA using human MELN and HeLa cells. -SCBT Publication Review
Date published: 2015-05-10
Rated 5 out of 5 by from Beautiful immunofluorescence nuclear stainingBeautiful immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-08
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DNA-PK CS 抗体 (G-4) is rated 4.6 out of 5 by 14.
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