Date published: 2025-9-8
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DNA Ligase IV Antibody (D-8) - Western Blotting - Image 361803
DNA 连接酶 IV 抗体 (D-8): sc-271299. DNA 连接酶 IV 在 HeLa (A), JAR (B) 和 KNRK (C) 全细胞裂解液以及大鼠胸腺组织提取物 (D) 中表达的 Western 印迹分析.
DNA 连接酶 IV 抗体 (D-8): sc-271299. DNA 连接酶 IV 在 HeLa (A), JAR (B) 和 KNRK (C) 全细胞裂解液以及大鼠胸腺组织提取物 (D) 中表达的 Western 印迹分析.

DNA Ligase IV 抗体 (D-8): sc-271299

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寫評論提問

说明书
  • DNA Ligase IV 抗体 D-8 是小鼠单克隆 IgG2a κ，DNA Ligase IV抗体, 在29篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列545-844位于human物种的DNA Ligase IV的C-terminus
  • DNA Ligase IV 抗体 (D-8) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 DNA Ligase IV
  • 抗DNA Ligase IV抗体（D-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取DNA Ligase IV (D-8): sc-271299的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2a BP-HRP">m-IgG2a BP-HRP是DNA Ligase IV Antibody (D-8) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与DNA Ligase IV Antibody (D-8) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外DNA Ligase抗体，包括DNA Ligase I, DNA Ligase III和DNA Ligase IV

DNA连接酶IV抗体（D-8）是一种IgG2a κ小鼠单克隆DNA连接酶IV抗体（也称为LIG4抗体、DNLI4抗体、多脱氧核糖核苷酸合成酶[ATP] 4抗体、连接酶IV DNA ATP依赖性抗体、多核苷酸连接酶抗体、DNA修复酶抗体、DNA连接酶抗体、密封酶抗体或LIG4S抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的DNA连接酶IV蛋白。DNA连接酶IV抗体（D-8）既有非偶联抗DNA连接酶IV抗体形式，也有多种偶联形式的抗DNA连接酶IV抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。X射线修复交叉互补蛋白XRCC4和DNA连接酶IV对于修复DNA中的双链断裂至关重要。这些蛋白质形成一个由两个分子组成的关键复合物，每个蛋白质分子优先与带有缺口或断裂末端的DNA结合。作为必需的辅助分子，XRCC4与DNA连接酶IV结合并增强其连接活性。XRCC4/DNA连接酶IV复合物还参与V(D)J重组。V(D)J重组发生在适应性免疫系统的正常发育过程中，涉及双链断裂中间体的形成。删除DNA连接酶IV或XRCC4中的任何一个都会抑制V(D)J重组的完成，导致发育中的神经系统凋亡发生率高，并阻断B细胞和T细胞的成熟。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DNA Ligase IV 抗体 (D-8) 参考文献:

  1. XR-1 细胞中 DNA 连接酶 IV 蛋白的缺失：XRCC4 稳定的证据。  |  Bryans, M., et al. 1999. Mutat Res. 433: 53-8. PMID: 10047779
  2. Xrcc4 蛋白的 DNA 结合与 V(D)J 重组有关，但与刺激 DNA 连接酶 IV 的活性无关。  |  Modesti, M., et al. 1999. EMBO J. 18: 2008-18. PMID: 10202163
  3. 一个参与 DNA 双链断裂修复和 V(D)J 重组的新基因位于人类 10p 染色体上。  |  Moshous, D., et al. 2000. Hum Mol Genet. 9: 583-8. PMID: 10699181
  4. DNA 连接酶 IV-XRCC4 复合物与 DNA 末端和 DNA 依赖性蛋白激酶的相互作用。  |  Chen, L., et al. 2000. J Biol Chem. 275: 26196-205. PMID: 10854421
  5. DNA 连接酶 IV 和 XRCC4 形成稳定的混合四聚体，在无细胞末端连接系统中与其他修复因子协同发挥作用。  |  Lee, KJ., et al. 2000. J Biol Chem. 275: 34787-96. PMID: 10945980
  6. Xrcc4 DNA 修复蛋白的晶体结构及其对末端连接的影响。  |  Junop, MS., et al. 2000. EMBO J. 19: 5962-70. PMID: 11080143
  7. 通过 RNA 干扰敲除 DNA 连接酶 IV/XRCC4 可抑制 I 型单纯疱疹病毒 DNA 复制。  |  Muylaert, I. and Elias, P. 2007. J Biol Chem. 282: 10865-72. PMID: 17296606
  8. 人类 XLF 与 DNA 的长度依赖性结合以及对 XRCC4.DNA 连接酶 IV 活性的刺激。  |  Lu, H., et al. 2007. J Biol Chem. 282: 11155-62. PMID: 17317666
  9. 一名 DNA 连接酶 IV (LIG4) 综合征患者的 Epstein-Barr 病毒相关 B 细胞淋巴瘤。  |  Toita, N., et al. 2007. Am J Med Genet A. 143A: 742-5. PMID: 17345618
  10. DNA连接酶IV缺乏：免疫球蛋白类缺乏取决于基因型。  |  Dard, R., et al. 2017. Pediatr Allergy Immunol. 28: 298-303. PMID: 28039949
  11. 人类DNA连接酶IV能够将NAD+作为DNA末端连接的替代性腺苷化供体。  |  Chen, SH. and Yu, X. 2019. Nucleic Acids Res. 47: 1321-1334. PMID: 30496552
  12. 非催化DNA连接酶IV促进活细胞中的DNA修复。  |  Goff, NJ., et al. 2022. Nucleic Acids Res. 50: 11058-11071. PMID: 36263813

订购信息

产品名称产品编号规格价格数量收藏夹

DNA Ligase IV 抗体 (D-8)

sc-271299
200 µg/ml
RMB2377.00

DNA Ligase IV (D-8): m-IgG2a BP-HRP 套装

sc-545930
200 µg Ab; 10 µg BP
RMB2662.00

DNA Ligase IV 抗体 (D-8) AC

sc-271299 AC
500 µg/ml, 25% agarose
RMB3129.00

DNA Ligase IV 抗体 (D-8) HRP

sc-271299 HRP
200 µg/ml
RMB2377.00

DNA Ligase IV 抗体 (D-8) FITC

sc-271299 FITC
200 µg/ml
RMB2482.00

DNA Ligase IV 抗体 (D-8) PE

sc-271299 PE
200 µg/ml
RMB2580.00

DNA Ligase IV 抗体 (D-8) Alexa Fluor® 488

sc-271299 AF488
200 µg/ml
RMB2685.00

DNA Ligase IV 抗体 (D-8) Alexa Fluor® 546

sc-271299 AF546
200 µg/ml
RMB2685.00

DNA Ligase IV 抗体 (D-8) Alexa Fluor® 594

sc-271299 AF594
200 µg/ml
RMB2685.00

DNA Ligase IV 抗体 (D-8) Alexa Fluor® 647

sc-271299 AF647
200 µg/ml
RMB2685.00

DNA Ligase IV 抗体 (D-8) Alexa Fluor® 680

sc-271299 AF680
200 µg/ml
RMB2685.00

DNA Ligase IV 抗体 (D-8) Alexa Fluor® 790

sc-271299 AF790
200 µg/ml
RMB2685.00

Your antibody against DNA Ligase IV (D-8): sc-271299 is indicated for immunoprecipitation. Do you have a publication where it has already been used successfully?

Asked by: Filippo
Thank you for your question. Please refer to publication PMID 27703167 for data on immunprecipitation using this antibody.
Answered by: Tech Support Europe
Date published: 2025-07-01

Can DNA Ligase IV (D-8): sc-271299 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, DNA Ligase IV (D-8): sc-271299 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from The antibody works.We acquired this antibody 7 months ago and probed on whole cell lysates of HeLa cells. We used a 1 to 1000 dilution with a 3 minute exposure. We saw a decent band. It works.
Date published: 2018-06-14
Rated 1 out of 5 by from Doesn't work at allIn our hands this antibody doesn't work in Western blotting. We see no detection of endogenous protein in U2OS and primary human fibroblasts, in fact it detects multiple bands, none of which are specific as adjudged by transfection with siRNA against DNA ligase IV.
Date published: 2017-11-27
Rated 5 out of 5 by from Good antibodyWorked well in our rat cell line. Used for WB at 1:1000 dilution, saw a clean band.
Date published: 2017-11-15
Rated 4 out of 5 by from Western BlotWorks great for WB at 1:1000 dilution in HeLa and 293T cells.
Date published: 2017-09-06
Rated 3 out of 5 by from Decent antibodyThis antibody worked well for westerns, however a few nonspecific bands that were quite dark.
Date published: 2017-06-22
Rated 5 out of 5 by from Specific and robust in WBGreat results in human and mouse samples on Western blot. Strong and specific.
Date published: 2017-01-26
Rated 4 out of 5 by from I used it for a western blot in 293T cellsI used it for a western blot in 293T cells. Worked pretty well!
Date published: 2015-10-19
Rated 5 out of 5 by from The DNA Ligase IV antibody (DThe DNA Ligase IV antibody (D-8) recognized one specific band around 110KD from the glioma cell LN229 RIPA lysate. It works great for Western blot.
Date published: 2015-09-10
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DNA Ligase IV 抗体 (D-8) is rated 3.9 out of 5 by 9.
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