Date published: 2025-9-7
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DLK1 Antibody (B-7) - Western Blotting - Image 301276
DLK1 抗体 (B-7): sc-376755. JAR 全细胞裂解液 (A) 和人胎盘组织提取物 (B) 中 DLK1 表达的 Western 印迹分析.
DLK1 抗体 (B-7): sc-376755. JAR 全细胞裂解液 (A) 和人胎盘组织提取物 (B) 中 DLK1 表达的 Western 印迹分析.

DLK1 抗体 (B-7): sc-376755

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说明书
  • DLK1 抗体 B-7 是小鼠单克隆 IgG3 κ，DLK1抗体, 在8篇文献中引用，规格为200 µg/ml
  • 免疫human物种的DLK的氨基酸266-383
  • 推荐用于 mouse, rat 和 human 来源的DLK1 WB, IP, IF, IHC(P) 和 ELISA检测
  • 另提供琼脂糖偶联抗体，规格为琼脂糖装，可用于IP实验；HRP 偶联抗体用于WB, IHC(P)和ELISA实验；以及藻红蛋白、FITC、Alexa Fluor® 488 或Alexa Fluor® 647 偶联抗体，规格为用于IF、IHC(P)和FCM实验
  • 关于如何获取DLK1 (B-7): sc-376755的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 DLK1 抗体 (B-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与DLK1 抗体 (B-7) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 DLK 抗体包括 DLK2

DLK1抗体（B-7）是一种IgG3 κ小鼠单克隆DLK1抗体（也称为DLK-1抗体、PREF1抗体、Delta1抗体、非典型Notch配体1抗体、含表皮生长因子重复序列的细胞生长相关跨膜蛋白抗体或ADAM17裂解依赖性成脂抑制剂抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类的DLK蛋白。DLK1抗体（B-7）既可作为非共轭抗DLK1抗体形式，也可作为多种共轭形式的抗DLK1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。人类DLK1基因定位于人类染色体14q32，并编码383个氨基酸的蛋白质。DLK1，也称为前脂肪细胞因子1（pref-1）、ZOG、pG2或FA1，是一种跨膜蛋白，在假定的细胞外结构域中具有六个串联的类EGF重复序列，这是类EGF蛋白家族的特征。DLK1与无脊椎动物同源异形蛋白（包括Delta和Notch）具有同源性，这些蛋白是果蝇中介导正常神经分化的蛋白质。在哺乳动物前脂肪细胞中，由于N-连接糖基化，存在多种离散的DLK1蛋白形式。DLK1在具有神经内分泌特征的肿瘤中表达，如神经母细胞瘤和嗜铬细胞瘤细胞系。正常组织表达仅限于肾上腺和胎盘。属于相同或不同细胞的DLK1蛋白之间的蛋白质-蛋白质相互作用，或可溶性DLK1变体与膜DLK1变体之间的相互作用，可能在调节DLK1功能中起重要作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DLK1 抗体 (B-7) 参考文献:

  1. 通过原位杂交将 DLK1 定位于人类染色体 14q32 带。  |  Gubina, E., et al. 1999. Cytogenet Cell Genet. 84: 206-7. PMID: 10393432
  2. dlk是一种参与分化的类表皮生长因子同源蛋白，其细胞外结构域的特定区域参与了分子内相互作用。  |  Baladrón, V., et al. 2001. Front Biosci. 6: A25-32. PMID: 11689349
  3. RPM-1和DLK-1通过控制Wnt信号调节先驱轴突的生长。  |  Park, EC. and Rongo, C. 2018. Development. 145: PMID: 30093552
  4. delta-like non-canonical Notch ligand 1 (DLK1) 在癌症中的作用。  |  Pittaway, JFH., et al. 2021. Endocr Relat Cancer. 28: R271-R287. PMID: 34627131
  5. 纤毛内运输依赖于负反馈调节 MAPKK DLK-1，以保护纤毛免于退化。  |  Sun, Y. and Jin, Y. 2023. Proc Natl Acad Sci U S A. 120: e2302801120. PMID: 37722038
  6. Dlk1-Dio3结构域的表观遗传控制和基因组印记动态。  |  Weinberg-Shukron, A., et al. 2023. Front Cell Dev Biol. 11: 1328806. PMID: 38155837
  7. 心包δ样非典型NOTCH配体1（Dlk1）通过上皮细胞向间充质的转化，促进心脏纤维化。  |  Jensen, CH., et al. 2024. Clin Transl Med. 14: e1565. PMID: 38328889
  8. 蛋白质基因组表面组研究发现神经母细胞瘤的免疫治疗靶点是 DLK1。  |  Hamilton, AK., et al. 2024. Cancer Cell. 42: 1970-1982.e7. PMID: 39454577
  9. 编码前脂肪细胞 EGF 样蛋白 pref-1 的基因的结构特征和交替剪接。  |  Smas, CM., et al. 1994. Biochemistry. 33: 9257-65. PMID: 7519443
  10. dlk, pG2 和 Pref-1 mRNA 编码属于 EGF 样超家族的类似蛋白。鉴定这种 RNA 的多态变体。  |  Lee, YL., et al. 1995. Biochim Biophys Acta. 1261: 223-32. PMID: 7711066
  11. dlk，一种在小细胞肺癌和神经内分泌肿瘤细胞系中差异表达的哺乳动物推测同源基因。  |  Laborda, J., et al. 1993. J Biol Chem. 268: 3817-20. PMID: 8095043
  12. Pref-1是一种含有类表皮生长因子重复序列的蛋白质，可抑制脂肪细胞分化。  |  Smas, CM. and Sul, HS. 1993. Cell. 73: 725-34. PMID: 8500166

订购信息

产品名称产品编号规格价格数量收藏夹

DLK1 抗体 (B-7)

sc-376755
200 µg/ml
RMB2377.00

DLK1 (B-7): m-IgGκ BP-HRP 套装

sc-523083
200 µg Ab, 40 µg BP
RMB2662.00

DLK1 抗体 (B-7) AC

sc-376755 AC
500 µg/ml, 25% agarose
RMB3129.00

DLK1 抗体 (B-7) Alexa Fluor® 488

sc-376755 AF488
200 µg/ml
RMB2685.00

DLK1 抗体 (B-7) Alexa Fluor® 647

sc-376755 AF647
200 µg/ml
RMB2685.00

DLK1 抗体 (B-7) FITC

sc-376755 FITC
200 µg/ml
RMB2482.00

DLK1 抗体 (B-7) HRP

sc-376755 HRP
200 µg/ml
RMB2377.00

DLK1 抗体 (B-7) PE

sc-376755 PE
200 µg/ml
RMB2580.00

What antigen retrieval method is recommended for human FFPE sample?

Asked by: Binbin
Thank you for contacting Santa Cruz Biotechnology. Please see section C of our protocol for antigen retrieval . https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining
Answered by: BlakeJ
Date published: 2025-01-23

DLK ANTIBODY IS THE SAME AS PREF1?

Asked by: MariaZ
Thank you for your question. Yes, DLK Antibody (B-7): sc-376755, is recommended for detection of DLK, which is also designated PREF1 (Pref-1), preadipocyte factor 1. The Gene Info and Protein Sequences can be found in tabs on this page below the "Related Products".
Answered by: Technical Support
Date published: 2019-05-02

Can DLK (B-7): sc-376755 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, DLK (B-7): sc-376755 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Works, but lots of backgroundI received DLK antibody as a free sample. While I did see a signal, I have quite a bit of background (as one of the pictures did). It worked for what I needed, but I wish the band definition had been clearer. I only ran it once, I could try to run it again and might not have background that time.
Date published: 2018-02-28
Rated 1 out of 5 by from IHCThe antibody does not work in mouse brain sections IHC. I used 1:100 dilution, even then nothing showed up.
Date published: 2018-02-09
Rated 5 out of 5 by from Works fine in WBThis antibody works well in WB with neuroblastma cells.
Date published: 2017-10-18
Rated 1 out of 5 by from Not good for IFTested on PFA fixed cryosections of embryonic mouse tissue. IF did not detect specific signal.
Date published: 2017-10-11
Rated 1 out of 5 by from Unfortunately did not work on mouse brain tissueImmunofluorescence (1:200) did not work on frozen section from 4% PFA fixed embryonic mouse brain tissue. 3-5 min retrieval with citric buffer (PH6.0).
Date published: 2017-10-11
Rated 4 out of 5 by from Werstern效果不错本人申请的是DLK小样试用装，在跑WB时，1:500的比例稀释，效果还可以。做过IF，一抗为·1:100，看不多荧光，不确定抗体问题，还是我本身细胞不表达DLK，目前正在尝试改良IF Protocol，再次进行验证
Date published: 2017-09-22
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of DLK expression in JAR whole cell lysate and human placenta tissue extract.
Date published: 2017-02-06
Rated 5 out of 5 by from Produced positive Western Blot data of DLKProduced positive Western Blot data of DLK expression in JAR whole cell lysate and human placenta tissue extract . -SCBT QC
Date published: 2015-03-08
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DLK1 抗体 (B-7) is rated 3.3 out of 5 by 9.
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