Date published: 2025-9-7
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DLC-1 Antibody (C-12) - Western Blotting - Image 130409
DLC-1 抗体 (C-12): sc-271915. MCF7 (A), ES-2 (B), SK-BR-3 (C) 和 KNRK (D) 全细胞裂解液中 DLC-1 表达的 Western 印迹分析.
DLC-1 Antibody (C-12) - Western Blotting - Image 130409
DLC-1 抗体 (C-12): sc-271915. MCF7 (A), ES-2 (B), SK-BR-3 (C) 和 KNRK (D) 全细胞裂解液中 DLC-1 表达的 Western 印迹分析.
DLC-1 Antibody (C-12) - Immunohistochemistry - Image 161040
DLC-1 抗体 (C-12): sc-271915. 福尔马林固定, 石蜡包埋的人胆囊组织的免疫过氧化物酶染色显示腺细胞的胞浆染色.
DLC-1 Antibody (C-12) - Western Blotting - Image 374905
DLC-1 抗体 (C-12): sc-271915. F9 (A) 和 U-251-MG (B) 全细胞裂解液中 DLC-1 表达的 Western 印迹分析.
DLC-1 抗体 (C-12): sc-271915. MCF7 (A), ES-2 (B), SK-BR-3 (C) 和 KNRK (D) 全细胞裂解液中 DLC-1 表达的 Western 印迹分析.

DLC-1 抗体 (C-12): sc-271915

4.8(12)
寫評論提問

说明书
  • DLC-1 抗体 C-12 是小鼠单克隆 IgG1 κ，DLC-1抗体, 在7篇文献中引用，规格为200 µg/ml
  • 针对氨基酸111-370的图谱，在DLC-1的内部区域，human起源
  • DLC-1 抗体 (C-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 DLC-1
  • 抗DLC-1抗体（C-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取DLC-1 (C-12): sc-271915的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 DLC-1 抗体 (C-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与DLC-1 抗体 (C-12) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

DLC 抗体

DLC-1抗体（C-12）是一种IgG1 κ小鼠单克隆DLC-1抗体（也称为DLC1抗体、RHG07抗体、DLC1 Rho GTPase激活蛋白抗体、ARHGAP7抗体、STARD12抗体、StAR相关脂质转移（START）结构域包含12抗体、Rho型GTPase激活蛋白7抗体、START结构域包含蛋白12抗体、肝癌中缺失的1蛋白抗体或P122-RhoGAP抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的DLC-1蛋白。DLC-1抗体（C-12）既有非偶联抗DLC-1抗体形式，也有多种偶联形式的抗DLC-1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。在大量人体组织（如乳腺、肝脏、结肠和前列腺）中，肝癌缺失蛋白1（DLC-1）的表达缺失与癌症表型密切相关，这通常是由于基因组缺失或启动子异常甲基化引起的。编码DLC-1的基因映射到人类染色体8p21-22上，该区域被认为是肿瘤抑制基因的所在地，因为它在许多癌症中经常发生突变。DLC-1定位于细胞质中，其表达的恢复会导致caspase-3介导的细胞凋亡，并抑制细胞生长和侵袭性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DLC-1 抗体 (C-12) 参考文献:

  1. DLC-1 基因可抑制人类乳腺癌细胞的生长和体内致瘤性。  |  Yuan, BZ., et al. 2003. Oncogene. 22: 445-50. PMID: 12545165
  2. 人类肝细胞癌候选抑癌基因 DLC-1 的 DNA 变异。  |  Park, SW., et al. 2003. Int J Oncol. 23: 133-7. PMID: 12792785
  3. 恢复 DLC-1 基因表达可诱导细胞凋亡，并抑制人肝癌细胞的生长和致瘤性。  |  Zhou, X., et al. 2004. Oncogene. 23: 1308-13. PMID: 14647417
  4. DLC-1 在人类非小细胞肺癌中是一种肿瘤抑制基因。  |  Yuan, BZ., et al. 2004. Oncogene. 23: 1405-11. PMID: 14661059
  5. 通过基因谱分析确定 ATF-3, caveolin-1, DLC-1 和 NM23-H2 为卵巢癌细胞的抗肿瘤, 受孕酮调控的假定基因。  |  Syed, V., et al. 2005. Oncogene. 24: 1774-87. PMID: 15674352
  6. DLC-1：一种 Rho GTPase 激活蛋白和肿瘤抑制因子。  |  Durkin, ME., et al. 2007. J Cell Mol Med. 11: 1185-207. PMID: 17979893
  7. 具有 RhoGAP 活性的肿瘤抑制蛋白 DLC-1 在调节细胞骨架和细胞运动中的作用。  |  Kim, TY., et al. 2009. Cancer Metastasis Rev. 28: 77-83. PMID: 19221866
  8. DLC-1通过靶向表皮生长因子受体/Akt/NF-κB通路诱导鼻咽癌线粒体凋亡和上皮间质转化停滞。  |  Huang, W., et al. 2015. Med Oncol. 32: 115. PMID: 25779535
  9. p122RhoGAP/DLC-1表达增强可导致冠状动脉痉挛  |  Kinjo, T., et al. 2015. PLoS One. 10: e0143884. PMID: 26624289
  10. 刚性诱导的内皮 DLC-1 表达通过稳定 ICAM-1 黏附体迫使白细胞扩散。  |  Schimmel, L., et al. 2018. Cell Rep. 24: 3115-3124. PMID: 30231995
  11. DLC-1 肿瘤抑制因子通过抑制 TGF-β1 信号传导调节人非小细胞肺癌细胞上 CD105 的表达。  |  Zhang, K., et al. 2020. Exp Cell Res. 386: 111732. PMID: 31770531
  12. 与大鼠 RhoGAP 同源的人类肝癌常缺失基因（DLC-1）的克隆, 特征和染色体定域。  |  Yuan, BZ., et al. 1998. Cancer Res. 58: 2196-9. PMID: 9605766

订购信息

产品名称产品编号规格价格数量收藏夹

DLC-1 抗体 (C-12)

sc-271915
200 µg/ml
RMB2377.00

DLC-1 (C-12): m-IgG Fc BP-HRP 套装

sc-527306
200 µg Ab; 10 µg BP
RMB2662.00

DLC-1 (C-12): m-IgG1 BP-HRP 套装

sc-532679
200 µg Ab; 20 µg BP
RMB2662.00

DLC-1 抗体 (C-12) AC

sc-271915 AC
500 µg/ml, 25% agarose
RMB3129.00

DLC-1 抗体 (C-12) HRP

sc-271915 HRP
200 µg/ml
RMB2377.00

DLC-1 抗体 (C-12) FITC

sc-271915 FITC
200 µg/ml
RMB2482.00

DLC-1 抗体 (C-12) PE

sc-271915 PE
200 µg/ml
RMB2580.00

DLC-1 抗体 (C-12) Alexa Fluor® 488

sc-271915 AF488
200 µg/ml
RMB2685.00

DLC-1 抗体 (C-12) Alexa Fluor® 546

sc-271915 AF546
200 µg/ml
RMB2685.00

DLC-1 抗体 (C-12) Alexa Fluor® 594

sc-271915 AF594
200 µg/ml
RMB2685.00

DLC-1 抗体 (C-12) Alexa Fluor® 647

sc-271915 AF647
200 µg/ml
RMB2685.00

DLC-1 抗体 (C-12) Alexa Fluor® 680

sc-271915 AF680
200 µg/ml
RMB2685.00

DLC-1 抗体 (C-12) Alexa Fluor® 790

sc-271915 AF790
200 µg/ml
RMB2685.00

In the datasheet it is claimed, that it is reactive to human, rat and mouse dlc1. However, the amino acids 111-370 (1-450) of human DLC1 are not present in either rat or mouse. Is there any validation for reactivity with these species?

Asked by: y27632
Thank you for your question. We do have Western blot data using this antibody in mouse, rat and human cell lines. For mouse and rat we have Western blot data in F9 and KNRK whole cell lysates, respectively. This antibody has also been published for use in mouse, rat and human. For an example of a publication using this antibody in mouse, along with the siRNA from Santa Cruz for mouse DLC-1, please see PMID: 30028837.
Answered by: Technical Support
Date published: 2019-10-17

转膜具体条件是什么?可以用半干转15V、45min吗？

Asked by: 帅123
Thank you for your inquiry. We transfer proteins onto a PVDF membrane overnight at 0.1 Amp. We use a transfer buffer composed of 50mM Tris, 380 mM glycine, 0.1% (w/v) SDS, 10% (v/v) methanol with a final pH of 8.3. For more information please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 9
Date published: 2019-01-03

I am using DLC-1 (C-12): sc-271915 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Trav11
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from DLC1抗体评论效果并不好，没有条带，我想要的条带在171kDa,但是没有检出来，嗯字符太短什么意思，还说啥，我操作没问题
Date published: 2017-11-07
Rated 5 out of 5 by from It works excellently.I tested it in PC3 cells at 1:200 dilution. Its band is below 130KD.
Date published: 2017-09-27
Rated 5 out of 5 by from DLC1（C-12）sc-271915抗体试用反馈DLC1（C-12）sc-271915，OSCC，1:500，条带略浅，1:200，条带合适效果较好
Date published: 2017-09-20
Rated 5 out of 5 by from good qualityThe antibody has good quality in Western blotting. With 1:300 dilution I get clear bands.
Date published: 2017-08-25
Rated 5 out of 5 by from great qualityso great quality . it works very well. The dilution when I used is 1:800.
Date published: 2017-05-28
Rated 5 out of 5 by from staining DLC-1DLC-1 antibody for IHC, we got having strong and specific staining.
Date published: 2017-04-24
Rated 5 out of 5 by from Work well with this antibodyThis antibody has good quality in Western blotting. With 1:1000 dilution I get clear bands, strong signals.
Date published: 2017-02-06
Rated 5 out of 5 by from Clean antibodyWorks well in Western Blot with minimal background, also shows good staining strength in IHC with paraffin-embedded sections.
Date published: 2017-01-26
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DLC-1 抗体 (C-12) is rated 4.8 out of 5 by 12.
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